Characterization of Physical Properties and Histologic Evaluation of Injectable Dermicol-P35 Porcine-Collagen Dermal Filler

Lorenc, Z. Paul; Nir, Eran; Azachi, Malkit

doi:10.1097/prs.0b013e3181d0ac75

Cited by 17 publications

(12 citation statements)

References 17 publications

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“…If the size of the cannula is too small for the material to expel easily, it will require high forces to expel which may be dangerous to patients and physicians. Studies have shown that increasing the cannula size and the required force for injection has a negative effect on the physician's ability to control the syringe [33] [34]. A study was conducted on the extrusion forces of syringes expelled by rheumatoid arthritis patients with hand impairment [35].…”

Section: Discussionmentioning

confidence: 99%

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Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications

Smith¹,

Snider²,

Gilley³

et al. 2017

JBNB

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A unique porcine extracellular matrix (ECM) derived injectable tissue construct with 100 nm or 20 nm gold nanoparticles (AuNP) was developed for musculoskeletal tissue engineering applications. ECM has been shown to encourage cellularity and tissue remodeling due to its release of growth factors while AuNP have been shown to reduce reactive oxygen species (ROS) levels. Injectable tissue constructs were created by homogenizing decellularized porcine diaphragm tendon conjugated with 100 nm or 20 nm AuNP at 1x, 4x, and 8x concentrations. Extrusion force testing demonstrated that homogenized tissue constructs were injectable at an appropriate cannula size and force. L-929 murine fibroblasts were used to measure cell viability, cell proliferation, intracellular ROS levels, and cell migration in response to constructs. Enhanced cell viability and proliferation are observed on 1 × 20 nm AuNP constructs. ROS assays demonstrate reduced cellular ROS concentrations from all 20 nm AuNP constructs and from 8 × 100 nm AuNP constructs compared with constructs without nanoparticles. Cellular migration is higher towards 4 × 20 nm AuNP constructs compared with constructs without nanoparticles. Results support the potential use of a porcine ECM derived injectable tissue construct with AuNP as an injectable tissue construct to reduce inflammation and to promote tissue remodeling in musculoskeletal tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

“…The values found in our study of ~1 N to 4 N are much less than those found in the cited study. In addition, the mean extrusion forces of porcine-collagen dermal fillers were measured to evaluate injectability [33]. The study found that the lowest collagen dermal filler had a mean extrusion force of 7.7 ± 0.5.…”

Section: Discussionmentioning

confidence: 99%

Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications

Smith¹,

Snider²,

Gilley³

et al. 2017

JBNB

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show abstract

“…Recently, Lorenc et al . () used Herovici's polychrome to evaluate the constructive remodelling of skin defects with porcine collagen dermal filler. These investigators showed that, as with the non‐crosslinked ECM scaffold in the present study, the porcine collagen promoted the de novo synthesis of new type I collagen within the implant with little or no encapsulation of the scaffold material, similar to our results with the non‐crosslinked ECM scaffold.…”

Section: Discussionmentioning

confidence: 99%

“…80% of the contractile force of uninjured muscle, a result that would indicate that, while ECM scaffold materials promote the regeneration of skeletal muscle, they minimize but do not eliminate the formation of scar tissue (Valentin et al, 2010). Recently, Lorenc et al (2010) used Herovici's polychrome to evaluate the constructive remodelling of skin defects with porcine collagen dermal filler. These investigators showed that, as with the non-crosslinked ECM scaffold in the present study, the porcine collagen promoted the de novo synthesis of new type I collagen within the implant with little or no encapsulation of the scaffold material, similar to our results with the non-crosslinked ECM scaffold.…”

Section: Discussionmentioning

confidence: 99%

Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling

Turner

Pezzone

Brown

et al. 2011

J Tissue Eng Regen Med

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Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling. Traditionally, the main method of assessing the host-material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. In the present study, the recently developed technique of multispectral imaging was used to revisit a little-described histological stain, Herovici's polychrome, which is capable of distinguishing between types I and III collagen. Combined, these techniques allowed quantification of collagen content and distribution of collagen types within a tissue sample. Samples of rat tail and human scar tissue were used to optimize the staining, while comparison with immunolabelled samples was used to develop a reproducible quantification system, based on the specific colour profiles for types I and III collagen. Finally the remodelling of rat abdominal wall defects repaired with crosslinked or non-crosslinked extracellular matrix scaffolds derived from porcine urinary bladder was assessed with this technique. Compared to standard histological assessment, the combination of multispectral imaging and Herovici's polychrome staining presents a quick, simple, reliable technique that can provide accurate quantification of tissue remodelling and specifically identify the expression and distribution of types I and III collagen.

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“…Fillers with porcine collagen (PC) have also been used with good cosmetic results and few adverse reactions. 38 It seems that PC does not require previous skin testing; it induces less inflammatory response than BC; histopathologic evaluation demonstrates that PC seems integrated within the host tissue, being difficult to distinguish by conventional histopathology between porcine and HC bundles (see Fig. 4B).…”

Section: Transitory Biodegradable or Resorbable Implantsmentioning

confidence: 96%

Foreign Body Granulomas

Molina-Ruiz¹,

Requena²

2015

Dermatologic Clinics

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Characterization of Physical Properties and Histologic Evaluation of Injectable Dermicol-P35 Porcine-Collagen Dermal Filler

Cited by 17 publications

References 17 publications

Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications

Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications

Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling

Foreign Body Granulomas

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