Characterization of Phytase Activity in Lupin Seed

Silva, L.G.; Trugo, L.C.

doi:10.1111/j.1745-4514.1996.tb00559.x

Cited by 16 publications

(17 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was found little changes in extractible P i in hazel seeds during chilling accompanied with IP6 mobilization that might be suggested the rapid conversion of P i into organic form [86]. These results were discussed as evidence of active metabolism in germinating seed [87]. In agreement, phytase is strongly and competitively inhibited by P i , while the decrease in phytase activity coincided with maximal IP6 turnover [88].…”

Section: Hydrolysis Of Phytic Acid During Seed Germinationmentioning

confidence: 63%

Metabolic Processes During Seed Germination

Ali¹,

Elozeiri²

2017

Advances in Seed Biology

129

102

View full text Add to dashboard Cite

Seed germination is crucial stage in plant development and can be considered as a determinant for plant productivity. Physiological and biochemical changes followed by morphological changes during germination are strongly related to seedling survival rate and vegetative growth which consequently affect yield and quality. This study is aimed to focus on proceeding of the most vital metabolic processes namely reserve mobilization, phytohormonal regulation, glyoxylate cycle and respiration process under either stressful or non-stressful conditions that may be led to suggest and conduct the more successful experimental improvements. Seed imbibition triggered the activation of various metabolic processes such as synthesis of hydrolytic enzymes which resulted in hydrolysis of reserve food into simple available form for embryo uptake. Abiotic stresses potentially affect seed germination and seedling establishment through various factors, such as a reduction in water availability, changes in the mobilization of stored reserves, hormonal balance alteration and affecting the structural organization of proteins. Recent strategies for improving seed quality involved classical genetic, molecular biology and invigoration treatments known as priming treatments. H 2 O 2 accumulation and associated oxidative damages together with a decline in antioxidant mechanisms can be regarded as a source of stress that may suppress germination. Seed priming was aimed primarily to control seed hydration by lowering external water potential, or shortening the hydration period.

show abstract

Section: Hydrolysis Of Phytic Acid During Seed Germinationmentioning

confidence: 63%

Metabolic Processes During Seed Germination

Ali¹,

Elozeiri²

2017

Advances in Seed Biology

129

102

View full text Add to dashboard Cite

show abstract

“…Na equação 3, observa-se que as variáveis tempo (x 1 ) e temperatura (x 2 ) de germinação influenciaram positivamente na atividade de fitase, enquanto que a interação (x 1 x 2 ) influenciou na diminuição da atividade de fitase. Várias investigações relataram que as condições de hidratação prévia e o tempo e temperatura de germinação de milho, soja, ervilhas, feijões e tremoço também foram importantes para obtenção de máxima atividade de fitase (LOLAS; MARKAKIS, 1977;CHANG;SHWIMMER;BURR, 1977;BEAL;METHA, 1985;BARTNIK;SZAFRANSKAA, 1987;LABOURE;GAGNON;LESCURE, 1993;HAMADA, 1996;SILVA;TRUGO, 1996).…”

Section: Resultsunclassified

Efeito das condições de germinação de girassol na redução do teor de fitato e ativação de fitase e fosfatase ácida

Agostini

Ida

2006

Sem. Ci. Agr.

View full text Add to dashboard Cite

ResumoA metodologia de superfície de resposta foi aplicada para investigar o efeito do tempo e temperatura do processo de germinação na redução do teor de fitato e na ativação das enzimas fitase e fosfatase ácida de girassol híbrido(Helianthus annuus) M734. Foi utilizado o delineamento fatorial 2 2 com duas repetições no ponto central com as variáveis tempo e temperatura em dois níveis, totalizando quatro experimentos com duas repetições no ponto central. Os experimentos foram conduzidos aleatoriamente e o procedimento de germinação foi realizado em câmara de germinação regulada a 25°C com iluminação fluorescente externa por oito dias. As sementes foram coletadas a partir do segundo dia, liofilizadas e moídas para análise de fitato e atividades de fitase e fosfatase ácida. Foi observado que o tempo e temperatura foram significativos na redução do teor de fitato e ocorrência de máxima atividades de fitase e fosfatase ácida na germinação do girassol híbrido. A maior redução do teor de fitato e máxima atividade de fosfatase ácida do girassol híbrido ocorreu no sexto dia de germinação a 30ºC. Entretanto, a máxima atividade de fitase ocorreu no quarto dia de germinação a 30ºC. Palavras-chave: Germinação de girassol, fitato, fitase, fosafatase ácida, metodologia de superfície de resposta AbstractThe response surface methodology was applied to investigate both length of time and temperature of the sunflower germination process on the phytate reduction and to enhancig phytase and acid phosphatase activities. The hybrid (Helianthus annuus) M734 was used and the factorial design 2 2 with two repetitions at the central point working with two variables time and temperature at two levels, completing four experiments with two repetitions at central point were applied. Experiments were performed randomly and the germination process was carried out using a germination camera at 25ºC under fluorescent illumination during 8 days. Seeds were collected from the second day of experiment, lyophilized and ground in order to process the quantitative analysis of phytate and the enzymes activity. Results showed that the length of time and temperature were significant on the reduction of phytate amount and the occurrence of the maximum enzymes activity. The maximum phytate reduction and the acid phosphatase activity was observed at the sixth day of seed germination at 30ºC while for phytase activity the best conditions were at the fourth day at the same temperature.

show abstract

“…Some of these compounds like lupin alkaloids are ascribed to have antimicrobial effects in order to protect the plant against herbivores and micro-organisms (Wink 1984;Erdemoglu et al 2007). The degradation of these substances may also occur due to plant endogenous enzymes like a-galactosidase or phytase (French 1954;Dey 1980;Dey et al 1983;Silva and Trugo 1996;Lahuta et al 2000). Other authors reported a reduced content of antinutritives after fermentation: according to Camacho et al (1991) fermentation of soaked and pasteurized lupin seeds with Lactobacillus acidophilus B-2092 and Lactobacillus buchneri B-1837 resulted in a decrease in phytates, whereas Lactobacillus acidophilus B-1910 and Lactobacillus fermentum B-585 reduced the oligosaccharide content.…”

Section: Introductionmentioning

confidence: 99%

“…However, no control without micro-organisms was run within this set of experiments in order to get a clear correlation with the microbial metabolism. The degradation of these substances may also occur due to plant endogenous enzymes like a-galactosidase or phytase (French 1954;Dey 1980;Dey et al 1983;Silva and Trugo 1996;Lahuta et al 2000).…”

Section: Introductionmentioning

confidence: 99%