Characterization of picroside II metabolites in rats by ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry

Gao, Tingting; Sheng, Tingting; Zhang, Tong; Han, Han

doi:10.1016/j.jpba.2016.06.005

Cited by 5 publications

(2 citation statements)

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“…Picroside II is known to have various beneficial effects on human health, including neuroprotective [40], hepatoprotective [41], antioxidation [42], and anti-inflammatory effects [11]. This study showed that picroside II ameliorated the severity of cerulein-induced SAP rats.…”

Section: Discussionmentioning

confidence: 76%

Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF‐κB‐Dependent Autophagy

Piao¹,

Liu

Guo

et al. 2017

Oxidative Medicine and Cellular Longevity

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Picroside II, from the herb Picrorhiza scrophulariiflora Pennell, has antioxidant and anti-inflammatory activities. However, its function on severe acute pancreatitis (SAP) and molecular mechanism remains unknown. The effects of picroside II on the SAP induced by cerulean were investigated. SAP rats were treated with picroside II (25 mg/kg). The severity of SAP was evaluated by using biochemical and histological analyses. Pancreatic cancer cell PANC-1 was transfected with ptfLC3 (an indicator of autophagic activity), pcDNA3.1-NF-κB (nuclear factor kappa B), and pTZU6+1-NF-κB-shRNA and then treated with picroside II. Relative molecules related with NF-κB-dependent autophagy were detected by using Western blot. Autophagic activities were observed by phase-contrast and fluorescent microscopes. Acetylated LC3 was detected by immunoprecipitation. The results showed that picroside II treatment reduced the level of ALT, AST, NF-κB, IL-1β, IL-6, TNF-α, and SIRT1 (NAD+-dependent deacetylase) and increased the level of SOD and GSH. The autophagic activity was reduced when NF-κB was silenced, and the levels of TNF-α and SIRT1 were reduced. In contrast, the overexpression of NF-κB increased autophagic activity and the level of TNF-α, which activated SIRT1. SIRT1 deacetylated LC3 and increased autophagic activities. Picroside II ameliorates SAP by improving antioxidant and anti-inflammtory activities of SAP models via NF-κB-dependent autophagy.

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Section: Discussionmentioning

confidence: 76%

Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF‐κB‐Dependent Autophagy

Piao¹,

Liu

Guo

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…Ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry (UPLC‐Q‐TOF/MS) is widely applied for analyzing both chemical components and profiling metabolites in TCMF (Zhong et al, ; Xu et al, ; Li, Zhang et al, ). A new data technique named UPLC‐Q‐TOF/MS E (where E stands for multiple collision energies), which is able to simultaneously obtain precursor ions and fragment ions in a single injection, has been developed and successfully used in recent years (Gao, Sheng, Zhang, & Han, ; Geng et al, ; Shi et al, ). Meanwhile, some relevant data processing softwarefor example, MetaboLynx™ and LightSight ® , equipped with mass defect filtering (MDF) function have been developed for the analysis of metabolites in a complex matrix.…”

Section: Introductionmentioning

confidence: 99%

The profiling and identification of chemical components, prototypes and metabolites of Run‐zao‐zhi‐yang capsule in rat plasma, urine and bile by an UPLC‐Q‐TOF/MS^E‐based high‐throughput strategy

Lin

Yue

Ou-Yang

et al. 2018

Biomedical Chromatography

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Run-zao-zhi-yang (RZZY) capsule, a traditional Chinese medicine formula, is popularly used for the treatment of dermatitis and eczema. However, few studies have been carried out on RZZY and its metabolites. In this study, we developed a three-step strategy to rapidly characterize the chemical constituents and metabolites of RZZY using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. A total of 41 chemical components were characterized from RZZY. Among these, there are 11 flavonoids, six alkaloids, six stilbene glycosides, five anthraquinones and 13 other compounds. In addition, 18 prototypes and 35 metabolites were detected in rat plasma, urine and bile. This study offers an applicable approach for high-throughput profiling and identification of chemical components and metabolites derived from traditional Chinese medicine formula in vivo, and also provides essential data for exploring bioactive ingredients and action mechanisms of RZZY.

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The beneficial pharmacological effects and potential mechanisms of picroside II: Evidence of its benefits from in vitro and in vivo

Wang

Lai

et al. 2020

Biomedicine & Pharmacotherapy

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Characterization of picroside II metabolites in rats by ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry

Cited by 5 publications

References 21 publications

Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF‐κB‐Dependent Autophagy

Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF‐κB‐Dependent Autophagy

The profiling and identification of chemical components, prototypes and metabolites of Run‐zao‐zhi‐yang capsule in rat plasma, urine and bile by an UPLC‐Q‐TOF/MS^E‐based high‐throughput strategy

The beneficial pharmacological effects and potential mechanisms of picroside II: Evidence of its benefits from in vitro and in vivo

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Characterization of picroside II metabolites in rats by ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry

Cited by 5 publications

References 21 publications

Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF‐κB‐Dependent Autophagy

Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF‐κB‐Dependent Autophagy

The profiling and identification of chemical components, prototypes and metabolites of Run‐zao‐zhi‐yang capsule in rat plasma, urine and bile by an UPLC‐Q‐TOF/MSE‐based high‐throughput strategy

The beneficial pharmacological effects and potential mechanisms of picroside II: Evidence of its benefits from in vitro and in vivo

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The profiling and identification of chemical components, prototypes and metabolites of Run‐zao‐zhi‐yang capsule in rat plasma, urine and bile by an UPLC‐Q‐TOF/MS^E‐based high‐throughput strategy