Characterization of Plant Antioxidative System in Response to Abiotic Stresses: A Focus on Heavy Metal Toxicity

Mourato, Miguel; Reis, Rafaela Amaral Dos; Martins, L. L.

doi:10.5772/34557

Cited by 66 publications

(55 citation statements)

References 97 publications

(97 reference statements)

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“…When NaCl was added to the irrigation solution simultaneously with Cd or Cu, MDA levels were reduced, which suggests protective role of NaCl against the stress caused by heavy metals. According to Mourato et al (2012), it can be indicated that the toxic effect of trace elements is probably exerted through free radical generation.…”

Section: Discussionmentioning

confidence: 99%

NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus

Bankaji¹,

Sleimi²,

Gómez-Cádenas

2017

Span. j. agric. res.

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The objective of the present work was to evaluate the extent of Cd-and Cu-induced oxidative stress and the antioxidant response triggered in the halophyte species Atriplex halimus after metallic trace elements exposure. Plants were treated for one month with Cd 2+ or Cu 2+ (400 µM) in the absence or presence of 200 mM NaCl in the irrigation solution. The interaction between salinity and heavy metal stress was analyzed in relation to plant growth, tissue ion contents (Na + , K + and Mg 2+ ), oxidative damage and antioxidative metabolism. Data indicate that shoot and root weight significantly decreased as a consequence of Cd 2+ -or Cu 2+ -induced stress. Metallic stress leads to unbalanced nutrient uptake by reducing the translocation of K + and Mg 2+ from the root to the shoot. The levels of malondialdehyde increased in root tissue when Cd, and especially Cu, were added to the irrigation solution, indicating that oxidative damage occurred. Results showed that NaCl gave a partial protection against Cd and Cu induced toxicity, although these contaminants had distinct influence on plant physiology. It can be concluded that salinity drastically modified heavy metal absorption and improved plant growth. Salinity also decreased oxidative damage, but differently in plants exposed to Cd or Cu stress.Additional key words: Atriplex halimus; oxidative damage; trace element toxicity; salinity Abbreviations used: APX (ascorbate peroxidase); CAT (catalase); DTNB (dinitrobenzoic acid); FW (fresh weight); GPX (guaiacol peroxidase); GSH (reduced glutathione); GSSG (oxidised glutathione); MDA (malondialdehyde); PC (phytochelatin); ROS (reactive oxygen species); SOD (superoxide dismutase); tGSH (total GSH) Authors' contributions: Cation determination assays: IB. Coordinated the obtention and culture of the plants and was responsible for conducting different experiments and the evaluation of plants: NS. Responsible of the study of the antioxidative system, evaluating the antioxidant enzymatic activities as well as MDA; he also was in charge of glutathione and phytochelatin content determination: AGC. Coordination of the works and preparing the manuscript: RMPC.Citation: Bankaji, I.; Sleimi, N.; Gómez-Cadenas, A.; Pérez-Clemente, R. M. (2016). NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus.

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Section: Discussionmentioning

confidence: 99%

NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus

Bankaji¹,

Sleimi²,

Gómez-Cádenas

2017

Span. j. agric. res.

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“…Extreme heavy metal toxicity frequently disrupts the preliminary protection strategies and the cellular redox system, causes enhanced production of reactive oxygen species (ROS) via oxidative stress. 35,114 Plant cells have developed two very essential antioxidant defence mechanisms. One such component involves the activation of antioxidant enzymes, such as superoxide dismutase (SOD), catalase, (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) for the direct inactivation of the reactive radicals.…”

Section: Heavy Metal Mediated Chemical Toxicity Generates Oxidative Amentioning

confidence: 99%

Oxidative and genotoxic damages in plants in response to heavy metal stress and maintenance of genome stability

Dutta

Mitra

Agarwal

et al. 2018

Plant Signaling & Behavior

110

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Plants, being sessile in nature, are constantly exposed to various environmental stresses, such as solar UV radiations, soil salinity, drought and desiccation, rehydration, low and high temperatures and other vast array of air and soil borne chemicals, industrial waste products, metals and metalloids. These agents, either directly or indirectly via the induction of oxidative stress and overproduction of reactive oxygen species (ROS), frequently perturb the chemical or physical structures of DNA and induce both cytotoxic or genotoxic stresses. Such condition, in turn, leads to genome instability and thus eventually severely affecting plant health and crop yield. With the growing industrialization process and non-judicious use of chemical fertilizers, the heavy metal mediated chemical toxicity has become one of the major environmental threats for the plants around the globe. The heavy metal ions cause damage to the structural, enzymatic and non-enzymatic components of plant cell, often resulting in loss of cell viability, thus negatively impacting plant growth and development. Plants have also evolved with an extensive and highly efficient mechanism to respond and adapt under such heavy metal toxicity mediated stress conditions. In addition to morpho-anatomical, hormonal and biochemical responses, at the molecular level, plants respond to heavy metal stress induced oxidative and genotoxic damage via the rapid change in the expression of the responsive genes at the transcriptional level. Various families of transcription factors play crucial role in triggering such responses. Apart from transcriptional response, epigenetic modifications have also been found to be essential for maintenance of plant genome stability under genotoxic stress. This review represents a comprehensive survey of recent advances in our understanding of plant responses to heavy metal mediated toxicity in general with particular emphasis on the transcriptional and epigenetic responses and highlights the importance of understanding the potential targets in the associated pathways for improved stress tolerance in crops.

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“…Phytotoxic concentrations of heavy metals are known to induce oxidative stress associated with enhancement in reactive oxygen species (ROS) production (11) that provoke antioxidant defense mechanisms (12). Beside provocation of ascorbate-glutathione cycle enzymes and other antioxidant enzymes [eg: superoxide dismutase (SOD, EC 1.15.1.1), peroxidases (POD; EC 1.11.1.7) and catalase (CAT; EC 1.11.1.6)], metabolic processes are directed towards biosynthetic pathways that consume reduction equivalents.…”

Section: Introductionmentioning

confidence: 99%

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

Fouad

Hafez

2018

Cell Mol Biol (Noisy-le-grand)

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The capabilities of cobalt ions (Co 2+) and cobalt nanoparticles (CoNPs) in enhancing alkaloids accumulation in Catharanthus roseus suspension cultures were evaluated in relation to the expression of CrMPK3 gene. Four concentrations (5, 10, 15 and 20 mg/L) of each cobalt form were utilized in addition to control. Both forms induced oxidative stress that was more pronounced for Co 2+ treatments. A positive correlation was observed between cobalt concentration and expression of CrMPK3 gene. However, a characteristic temporal expression profile was recorded for each cobalt form. Also, positive correlations were detected between both cobalt concentration and expression of CrMPK3 gene on one hand and the activities of antioxidant enzymes (SOD and APX) and alkaloids content on the other hand. Such correlations suggest CrMPK3 gene as a common player in cobalt-induced stress signaling; regardless of cobalt form. Results revealed the higher capability of Co 2+ , compared with CoNPs, in enhancing alkaloids accumulation. However, results supported CoNPs as a novel tool in manipulating cobaltinduced alkaloids production in C. roseus.

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Characterization of Plant Antioxidative System in Response to Abiotic Stresses: A Focus on Heavy Metal Toxicity

Cited by 66 publications

References 97 publications

NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus

NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus

Oxidative and genotoxic damages in plants in response to heavy metal stress and maintenance of genome stability

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

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