Characterization of plant growth-promoting traits of bacteria isolated from the rhizosphere of grapevine grown in alkaline and acidic soils

Karagöz, Kenan; Ateş, Fadime; Karagöz, Halit; Kotan, Recep; Çakmakçı, Ramazan

doi:10.1016/j.ejsobi.2012.01.007

Cited by 65 publications

(41 citation statements)

References 41 publications

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“…The remaining Gram-positive isolates were affiliated to the Firmicutes (31%), Actinobacteria (2%), and Bacteroidetes (1%) classes (Figure 4(a)). Members of these taxa have been found to be associated with other grape plants cultivated in Italy [9, 57], France [4, 63], Turkey [64], Nova Scotia [7], and Australia [8]. A high Shannon-Weaver diversity index, calculated from the number of individuals per genus, was found within the rhizosphere of grapes cultivated in Italy ( H ′ = 1.52) and Tunisia ( H ′ = 1.36) and in the endosphere of ungrafted Barbera in Italy ( H ′ = 1.37).…”

Section: Resultsmentioning

confidence: 99%

Plant Growth Promotion Potential Is Equally Represented in Diverse Grapevine Root-Associated Bacterial Communities from Different Biopedoclimatic Environments

Marasco

Rolli

Fusi

et al. 2013

BioMed Research International

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Plant-associated bacteria provide important services to host plants. Environmental factors such as cultivar type and pedoclimatic conditions contribute to shape their diversity. However, whether these environmental factors may influence the plant growth promoting (PGP) potential of the root-associated bacteria is not widely understood. To address this issue, the diversity and PGP potential of the bacterial assemblage associated with the grapevine root system of different cultivars in three Mediterranean environments along a macrotransect identifying an aridity gradient were assessed by culture-dependent and independent approaches. According to 16S rRNA gene PCR-DGGE, the structure of endosphere and rhizosphere bacterial communities was highly diverse (P = 0.03) and was associated with a cultivar/latitudinal/climatic effect. Despite being diverse, the bacterial communities associated with Egyptian grapevines shared a higher similarity with the Tunisian grapevines than those cultivated in North Italy. A similar distribution, according to the cultivar/latitude/aridity gradients, was observed for the cultivable bacteria. Many isolates (23%) presented in vitro multiple stress resistance capabilities and PGP activities, the most frequent being auxin synthesis (82%), insoluble phosphate solubilisation (61%), and ammonia production (70%). The comparable numbers and types of potential PGP traits among the three different environmental settings indicate a strong functional homeostasis of beneficial bacteria associated with grape root.

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Section: Resultsmentioning

confidence: 99%

Plant Growth Promotion Potential Is Equally Represented in Diverse Grapevine Root-Associated Bacterial Communities from Different Biopedoclimatic Environments

Marasco

Rolli

Fusi

et al. 2013

BioMed Research International

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“…One of the main contributors to the solubilization of phosphate in the soil is its reduced pH value caused by the bacteria's production of organic acids (Karagöz et al, 2012). In terms of siderophores, according to Benite, Machado, and Machado (2002), in the last three decades, over one hundred naturally occurring siderophores have been isolated and characterized, including those of bacterial (Streptomyces, E. coli, Pseudomonas, Bacillus, Micobacterium) and fungal origin (Aspergillus, Penicillium).…”

Section: Discussionmentioning

confidence: 99%

“…Ahmad, I. Ahmad, & Khan, 2008;Zahir, Asghar, Akhtar, & Arshad, 2005;Asghar, Zahir, Arshad, & Khaliq, 2002). Although several studies have reported the potential of different microorganisms to promote growth in plants such as wheat, soybeans, and potatoes, these are in short supply for the cultivation of grapevines, both in terms of the isolation of the microorganisms and when it comes to in vitro and in vivo testing (Dawwam, Elbeltagia, Emara, Abbas, & Hassan, 2013;Karagöz, Ates, Karagöz, Kotan, & Cakmakci, 2012;Smyth et al, 2011;Khalid, Arshad, & Zahir, 2004). In the last couple of decades, research efforts related to the development of biological consumables, such as inoculating agents, have mustered a lot of attention from researchers.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization and Selection of Bacteria that Promote Plant Growth in Grapevines (Vitis sp.)

Biasolo¹,

Kucmanski²,

Salamoni³

et al. 2016

JAS

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Certain bacteria can promote and stimulate plant growth, increasing the production of biomass and reducing damage caused by phytopathogens. With that in mind, this research effort set out to select these plant growth-promoting bacteria in order to evaluate their effects on the growth of grapevines (Vitis sp.). The bacteria were isolated from several vineyard soil samples, and evaluated based on their production of IAA (Indole-3-Acetic Acid), siderophores and cellulase, as well as their phosphate solubilization and nitrogen fixation capabilities. In vivo testing included six separate treatments with the following bacterial isolates: C12, O7, B3, I3, a control group and a blended group. The tests were performed in a greenhouse with bacterial suspension inoculation placed around the roots of Paulsen 1103 rootstock cuttings. The data collected included the following: number of leaves per plant, branch lengths, chlorophyll content, fresh and dry mass, and Carbon, Hydrogen, Nitrogen and Sulfur concentrations. Forty-six separate bacteria were isolated, of which 100% produced IAA, 65.21% produced siderophores, 63.04% solubilized phosphate, 34.78% produced cellulase, and 30.43% showed nitrogen fixation. The in vivo testing also revealed significant increases in the length of the branch and in percentages of Carbon and Nitrogen. The C12 isolate exhibited the highest increase in branch length (76.704 cm), whereas the O7 and C12 were identified as Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively.

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“…However, little is known about the prevalence of PSB in the rhizospheres of grapevines. Karagöz et al (2012) isolated 17 PSB strains from the rhizosphere soil of grapevines in Turkey. Marasco et al (2013) assessed the diversity and plant-growth-promoting (PGP) potential of the bacteria associated with the grapevine root system of different cultivars in three Mediterranean environments, and found that in all the strains isolated, including rhizobacteria and endophytic bacteria, 61% of them were able of solubilizing insoluble phosphate.…”

Section: Isolation and Screening Of Psbmentioning

confidence: 99%

“…Approximately 10 g of the soil adhering to the roots of each individual grape plant, considered the rhizospheric soil, was used for the bacterial isolation procedures. Plant residues and stones were removed, and the fresh soil samples were stored in sealed sterile bags at 4°C (Karagöz et al, 2012).…”

Section: Sample Collectionmentioning

confidence: 99%

Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

Ling

Liu

Cheng

et al. 2017

Span. j. agric. res.

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Phosphate-solubilizing bacteria (PSB) have the ability to solubilize insoluble phosphorus (P) and release soluble P. Extensive research has been performed with respect to PSB isolation from the rhizospheres of various plants, but little is known about the prevalence of PSB in the grapevine rhizosphere. In this study, we aimed to isolate and identify PSB from the grapevine rhizosphere in five vineyards of Northwest China, to characterize their plant-growth-promoting (PGP) traits, evaluate the effect of stress on their phosphate-solubilizing activity (PSA), and test their ability to stimulate the growth of Vitis vinifera L. cv. Cabernet Sauvignon. From the vineyard soils, 66 PSB isolates were screened, and 10 strains with high PSA were identified by 16S rRNA sequencing. Sequence analysis revealed that these 10 strains belonged to 4 genera and 5 species: Bacillus aryabhattai, B. megaterium, Klebsiella variicola, Stenotrophomonas rhizophila, and Enterobacter aerogenes. The selected PSB strains JY17 (B. aryabhattai) and JY22 (B. aryabhattai) were positive for multiple PGP traits, including nitrogen fixation and production of indole acetic acid (IAA), siderophores, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, chitinase, and protease. JY17 and JY22 showed strong PSA under stress conditions of high pH, high salt, and high temperature. Therefore, these two isolates can be used as biofertilizers in saline-alkaline soils. The inoculation with PSB significantly facilitated the growth of V. vinifera cv. Cabernet Sauvignon under greenhouse conditions. Use of these PSB as biofertilizers will increase the available P content in soils, minimize P-fertilizer application, reduce environmental pollution, and promote sustainable agriculture.Additional key words: promote plant growth; inorganic phosphate; stress conditions; saline-alkaline soil; Vitis vinifera L. cv. Cabernet SauvignonAbbreviations used: ACC (1-aminocyclopropane-1-carboxylate); CAS (chrome azurol S); DF (Dworkin-Foster); GADH (gluconate dehydrogenase); IAA (indole acetic acid); NBRIP (National Botanical Research Institute's phosphate); PGP (plant-growthpromoting); PQQ-GDH (pyrroloquinoline quinone-dependent glucose dehydrogenase); PSA (phosphate-solubilizing activity); PSB (phosphate-solubilizing bacteria); TCP (tricalcium phosphate).Authors' contributions: ML drafted the manuscript; XL made critical revision of the manuscript for important intellectual content; BSC and XLM acquired, analyzed and interpreted data; XTL coordinated the research project; XFZ performed statistical analysis; YLJ and ZM proposed revision suggestions; YLF supervised the work.Citation: Liu, M.; Liu, X.; Cheng, B. S.; Ma, X. L.; Lyu, X. T.; Zhao, X. F.; Ju, Y. L.; Min, Z.; Fang, Y. L. (2016). Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers.

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Characterization of plant growth-promoting traits of bacteria isolated from the rhizosphere of grapevine grown in alkaline and acidic soils

Cited by 65 publications

References 41 publications

Plant Growth Promotion Potential Is Equally Represented in Diverse Grapevine Root-Associated Bacterial Communities from Different Biopedoclimatic Environments

Plant Growth Promotion Potential Is Equally Represented in Diverse Grapevine Root-Associated Bacterial Communities from Different Biopedoclimatic Environments

Isolation, Characterization and Selection of Bacteria that Promote Plant Growth in Grapevines (Vitis sp.)

Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

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