Characterization of Pm68, a new powdery mildew resistance gene on chromosome 2BS of Greek durum wheat TRI 1796

He, Huagang; Liu, Renkang; Ma, Pengtao; Du, Haonan; Zhang, Huan‐Huan; Wu, Qiuhong; Yang, Lijun; Gong, Shuangjun; Liu, Tianlei; Huo, Naxin; Gu, Yong; Zhu, Shanying

doi:10.1007/s00122-020-03681-2

Cited by 92 publications

(43 citation statements)

References 33 publications

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“…Various scientists have been committed to discovering new loci or alleles conferring resistance to powdery mildew, and, to date, 99 Pm genes at 60 loci ( Pm1–Pm68 ) that confer resistance to powdery mildew have been publicly reported [ 7 , 8 , 9 , 10 ]; these genes have been assigned to almost all chromosomes except 3D and 4D. Unfortunately, only a few Pm genes, such as Pm21, have been successfully used in the development of resistant cultivars because of the loss of resistance resulted from the rapid variation in Bgt races [ 11 ], linkage drag [ 12 , 13 ], and the shortage of information on both the sequence and function of Pm genes [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping

Yang

Zhong

Chen

et al. 2021

IJMS

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Wheat powdery mildew, caused by the obligate parasite Blumeria graminis f. sp. tritici, severely reduces wheat yields. Identifying durable and effective genes against wheat powdery mildew and further transferring them into wheat cultivars is important for finally controlling this disease in wheat production. Pm40 has been widely used in wheat breeding programs in Southwest China due to the spectrum and potentially durable resistance to powdery mildew. In the present study, a resistance test demonstrated that Pm40 is still effective against the Bgt race E20. We identified and cloned the TraesCS7B01G164000 with a total length of 4883 bp, including three exons and two introns, and encoded a protein carrying the CC-NBS-NBS-LRR domain in the Pm40-linked region flanked by two EST markers, BF478514 and BF291338, by integrating analysis of gene annotation in wheat reference genome and both sequence and expression difference in available transcriptome data. Two missense mutations were detected at positions 68 and 83 in the CC domain. The results of both cosegregation linkage analysis and qRT-PCR also suggested that TraesCS7B01G164000 was a potential candidate gene of Pm40. This study allowed us to move toward the final successfully clone and apply Pm40 in wheat resistance improvement by gene engineering.

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Section: Introductionmentioning

confidence: 99%

Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping

Yang

Zhong

Chen

et al. 2021

IJMS

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“…So far, there are 68 (Pm1 to Pm68) powdery mildew resistance genes and 79 (Lr1 to Lr79) leaf rust resistance genes which have been cataloged in wheat and its relatives (Qureshi et al, 2018;He et al, 2021). However, many of them are overcome by new virulent pathogens.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust

Wang

Liu

et al. 2021

Front. Plant Sci.

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Thinopyrum ponticum (2n = 10x = 70) is a wild relative of wheat with high tolerance to both biotic and abiotic stresses; it has been wildly used in wheat genetic improvement. A disomic substitution line named SN19647 was derived from a cross between Triticum aestivum and the wheat–Th. ponticum partial amphiploid SNTE20 (2n = 8x = 56). It was evaluated for disease resistance and characterized via sequential fluorescence in situ hybridization (FISH)-genomic in situ hybridization (GISH) and molecular markers. The results showed that SN19647 carried resistance to both powdery mildew and leaf rust. It contained 42 chromosomes with a pair of wheat chromosome 1B replaced by a pair of JS chromosomes from Th. ponticum. In addition to chromosomal substitution events, structural variation also occurred on wheat chromosomes 2A, 5A, 6B, and 7B. Based on marker analysis, 19 markers specific to the JS chromosome were obtained, of which seventeen markers belonged to homoeologous group one. These results indicated that SN19647 was a 1JS (1B) substitution line. Compared with the known 1JS (1D) substitution line CH10A5, it was found that 17 markers generated different specific bands to Th. ponticum, confirming the novelty of the 1JS chromosome in SN19647. Therefore, SN19647, resistant to powdery mildew and leaf rust, was a novel 1JS (1B) substitution line that can be used in wheat genetic improvement.

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“…The development of resistant cultivars relies on the facts that there are abundant resistance gene resources. So far, more than 100 formally and 30 temporarily designated powdery mildew resistance (Pm) genes/alleles have been identi ed at 63 loci within common wheat and its diverse relatives (McIntosh et al 2020;He et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Together, the two forms of resistance have provided the genetic basis of powdery mildew resistance in wheat. Due to ease of selection during breeding, the focus has been on the Pm genes providing qualitative resistance but, as mentioned above, many Pm genes with qualitative resistance have lost their resistance to the powdery mildew plant-pathogen interaction (Ma et al 2015;He et al 2021). Consequently, there is an urgent need to mine and utilize more effective resistance sources to increase the genetic diversity of Pm genes.…”

Section: Introductionmentioning

confidence: 99%

Genetic Dissection of the Powdery Mildew Resistance in Wheat Breeding Line LS5082 Using BSR-Seq

Zhu

et al. 2021

Preprint

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Wheat powdery mildew is a destructive disease seriously threatening yield and quality. Comprehensive dissection of new resistance-related loci/genes is necessary to control this disease. LS5082 is a Chinese wheat breeding line with resistance to powdery mildew. Genetic analysis indicated that a single dominant gene, tentatively designated PmLS5082, conferred seedling resistance to different Bgt isolates. Bulked segregant RNA-seq (BSR-seq) was carried out to map the R gene PmLS5082 and profile differentially expressed genes associated with PmLS5082. PmLS5082 was mapped to chromosome arm 2BL and flanked by the markers WGGBH612-5 and YTU19-005 with genetic distances of 0.3 and 0.4 cM, respectively. The physical position was subsequently locked into the interval of 710.3-711.0 Mb. PmLS5082 differs from the catalogued Pm genes on chromosome arm 2BL in its resistant spectrum, physical position and origin, suggesting it is most likely a new Pm gene. Through COG and KEGG analysis, differentially expressed genes associated with PmLS5082 were profiled, and six genes in PmLS5082 interval were confirmed to be associated with PmLS5082 via RT-qPCR, using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. In order to transfer PmLS082 to different genetic backgrounds using marker-assisted selection (MAS), closely linked markers, including Kompetitive Allele-Specific PCR (KASP) markers, were evaluated and nine markers were confirmed to be widely applicable for MAS in different genetic backgrounds.

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Characterization of Pm68, a new powdery mildew resistance gene on chromosome 2BS of Greek durum wheat TRI 1796

Cited by 92 publications

References 33 publications

Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping

Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping

Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust

Genetic Dissection of the Powdery Mildew Resistance in Wheat Breeding Line LS5082 Using BSR-Seq

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