Characterization of pollen antigen–induced IL-31 production by PBMCs in patients with allergic rhinitis

Okano, Mitsuhiro; Fujiwara, Tazuko; Higaki, Toru; Makihara, Seiichiro; Tamano, Haruna; Noda, Yoichi; Kanai, Kazuaki; Kariya, Shin; Yasueda, Hiroshi; Nishizaki, Kazunori

doi:10.1016/j.jaci.2010.09.029

Cited by 36 publications

(27 citation statements)

References 19 publications

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“…This nonsignificant difference in IL-31 serum level may be considered to be similar to results reported by other authors who concluded that IL-31 has a unique and independent role in the pathophysiology of allergic rhinitis [14].…”

Section: Discussionsupporting

confidence: 91%

Determination of interleukin 31 (IL-31) serum levels in allergic rhinitis patients

Ashari

Amin

Hamid

et al. 2014

International Journal of Pediatrics and Adolescent Medicine

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Background and objectives: IL-31 appears to be an important regulator of Th2 responses and was found to be present in patients with atopic dermatitis. This study was conducted to compare IL-31 serum levels in allergic rhinitis (AR) patients and normal controls and to determine IL-31 serum levels according to AR severity. Patients and methods: A cross-sectional study was conducted with AR patients who met the Allergic Rhinitis and its Impact on Asthma (ARIA) classification criteria for AR. Normal control subjects were recruited from local healthy people in HUSM. The severity of AR was assessed using ARIA. Five ml of blood were drawn from each subject. The serum samples were analyzed for IL-31 using ELISA kits. Results: The results showed that IL-31 serum level was higher in allergic rhinitis patients (mean (SD) 4107.70 (16961.51)) than in normal controls (2195.55 (9016.57)); however, this difference was not statistically significant, with a p value of 0.406 determined by an independent-T test. The results also showed no significant difference in IL-31 levels according to AR severity, with a p value of 0.245 determined by a ManneWhitney test. Conclusion: There was no significant difference in IL-31 serum levels between normal controls and AR patients or between patients with different AR severities. Future studies with larger sample sizes should be performed.

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Section: Discussionsupporting

confidence: 91%

Determination of interleukin 31 (IL-31) serum levels in allergic rhinitis patients

Ashari

Amin

Hamid

et al. 2014

International Journal of Pediatrics and Adolescent Medicine

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“…IL-31 as a multifunctional protein has been investigated in allergy and asthma until recently (11,12). A number of studies have shown enhanced IL-31 expression by PBMCs and local nasal mucosa in AR and asthma patient (14,15). However, the regulation and expression in AR children was not known.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-31 promotes helper T cell type-2 inflammation in children with allergic rhinitis

et al. 2014

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Background: Interleukin-31 (IL-31) is a recently described cytokine that is involved in helper T cell type-2 (Th2)-mediated diseases. However, its regulatory effect in the pathogenesis of children allergic rhinitis (AR) needs to be further characterized. This study sought to evaluate the expression and role of IL-31 in children with AR. Methods: Sixty children with AR and 20 normal controls were included. IL-31 and Th2 cytokines production in tissue, serum, and nasal lavage was examined by immunohistochemistry, quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay, respectively. Peripheral blood mononuclear cells (PBMCs) were purified for in vitro regulation experiment of IL-31. Nasal epithelial cells (NECs) were cultured and stimulated by recombinant IL-31. results: The IL-31 mRNA and protein levels in both serum and nasal lavage were significantly enhanced in AR compared with normal controls, especially in children with asthma. The nasal IL-31 was associated with enhanced local Th2 cytokines and mucin 5AC (MUC5AC) expression. In vitro study showed that IL-31 promotes Th2 cytokines expression and MUC5AC upregulation and thus amplified Th2 inflammation. conclusion: Our results demonstrate that IL-31 expression in AR aggravated and amplified Th2 inflammation as well as mucin production, and provide a possible explanation for IL-31's regulatory role in the pathogenesis of AR. a llergic rhinitis (AR) is a common nasal disease and affects 10-40% of children with increasing prevalence (1,2). AR frequently coexists with asthma (most studies report a rate of 50-60%) in children (3). The major histological features of AR include helper T cell type-2 (Th2) inflammation accompanied by infiltration with eosinophils, allergen-specific IgE production, and thickening of the basement membrane (4,5). Activation of Th2 cells and further released cytokines induced accumulation of inflammatory cells, such as B cells, plasma cells, eosinophils, basophils, and neutrophils, which contributed to the occurrence of AR (6). Despite involvement of Th2 inflammation in AR was clearly clarified, its regulation was not fully understood.Interleukin-31 (IL-31) is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4 + T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on (7). The receptor of IL-31 consists of IL-31 receptor A (IL-31RA) and the oncostatin M receptor that is constitutively expressed on epithelial cells (8). The role of IL-31 in allergic diseases mainly focused on atopic dermatitis. Stephan et al. reported elevated IL-31 expression in atopic dermatitis (9). UIrike et al. found a correlation of Th2 cytokines with increased level of IL-31 in children with extrinsic atopic dermatitis (10). However, studies on respiratory allergic diseases are relatively few. Recently, Lei et al. (11) reported that high IL-31 level in asthmatic subjects, suggesting that IL-31 ...

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“…However, recent studies imply that IL-31 might also be involved in allergic asthma and rhinitis [150][151][152], in inflammatory bowel disease [144] and malignancies of hematopoietic and lymphoid tissues [119,134,153]. Recent reviews summarize these findings in detail [123,124,154].…”

Section: Animal Models Investigating Il-31 Physiology and Recent Findmentioning

confidence: 99%

Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology

Hermanns

2015

Cytokine & Growth Factor Reviews

202

239

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Characterization of pollen antigen–induced IL-31 production by PBMCs in patients with allergic rhinitis

Cited by 36 publications

References 19 publications

Determination of interleukin 31 (IL-31) serum levels in allergic rhinitis patients

Determination of interleukin 31 (IL-31) serum levels in allergic rhinitis patients

Interleukin-31 promotes helper T cell type-2 inflammation in children with allergic rhinitis

Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology

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