Characterization of Polyphosphate Glucokinase SCO5059 from<i>Streptomyces coelicolor</i>A3(2)

Koide, Mai; Miyanaga, Akimasa; Kudo, Fumitaka; Eguchi, Tadashi

doi:10.1271/bbb.130498

Cited by 11 publications

(7 citation statements)

References 18 publications

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“…These proteins use glucose and polyP (and often nucleotides such as ATP) as substrates to catalyze glucose phosphorylation, generating glucose 6-phosphate. PPGKs were first observed in Mycobacterium phlei ( Szymona, 1957 ) and in Gram-positive bacteria in the order Actinomycetales ( Szymona and Ostrowski, 1964 ; Szymona and Widomski, 1974 ; Szymona and Szymona, 1978 ; Szymona and Szymona, 1979 ; Pepin and Wood, 1986 ; Mukai et al., 2003 ; Tanaka et al., 2003 ; Lindner et al., 2010 ; Liao et al., 2012 ; Koide et al., 2013 ). Although many PPGK enzymes can use a range of nucleotide triphosphates as substrates, the enzymes of the most ancient Actinomycetales species have a preference for polyP.…”

Section: Polyp Functions and Regulation In Photosynthetic Microbesmentioning

confidence: 99%

Polyphosphate: A Multifunctional Metabolite in Cyanobacteria and Algae

2020

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Polyphosphate (polyP), a polymer of orthophosphate (PO 4 3-) of varying lengths, has been identified in all kingdoms of life. It can serve as a source of chemical bond energy (phosphoanhydride bond) that may have been used by biological systems prior to the evolution of ATP. Intracellular polyP is mainly stored as granules in specific vacuoles called acidocalcisomes, and its synthesis and accumulation appear to impact a myriad of cellular functions. It serves as a reservoir for inorganic PO 4 3and an energy source for fueling cellular metabolism, participates in maintaining adenylate and metal cation homeostasis, functions as a scaffold for sequestering cations, exhibits chaperone function, covalently binds to proteins to modify their activity, and enables normal acclimation of cells to stress conditions. PolyP also appears to have a role in symbiotic and parasitic associations, and in higher eukaryotes, low polyP levels seem to impact cancerous proliferation, apoptosis, procoagulant and proinflammatory responses and cause defects in TOR signaling. In this review, we discuss the metabolism, storage, and function of polyP in photosynthetic microbes, which mostly includes research on green algae and cyanobacteria. We focus on factors that impact polyP synthesis, specific enzymes required for its synthesis and degradation, sequestration of polyP in acidocalcisomes, its role in cellular energetics, acclimation processes, and metal homeostasis, and then transition to its potential applications for bioremediation and medical purposes.

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Section: Polyp Functions and Regulation In Photosynthetic Microbesmentioning

confidence: 99%

Polyphosphate: A Multifunctional Metabolite in Cyanobacteria and Algae

2020

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“…Based on the experimental results, we speculated that extreme temperatures could partially inactivate the enzyme. Mai et al found that polyphosphate kinase had the highest enzymatic activity at around 30 • C [10]. The reaction was slightly inhibited when the temperature was 20 • C and 40 • C. The highest conversion was seen at 30 • C, reaching 85.46% conversion efficiency.…”

Section: Optimization Of Temperaturementioning

confidence: 99%

The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase

et al. 2019

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Mannose-6-phosphate (M6P) is involved in many metabolic pathways in life, and it has important applications in the treatment of diseases. This study explored a cost-effective enzyme catalytic synthesis method of M6P, using polyphosphate-dependent mannose kinase from Arthrobacter species. This synthesis uses polyphosphate to replace expensive ATP, and it is greener and safer than chemical synthesis. This study investigated the effects of key factors such as metal ions, temperature, and substrate addition on this enzymatic reaction, and improved the conversion efficiency. We moreover take advantage of the response surface method to explore the best catalytic conditions synthetically. The conversion was 99.17% successful under the optimal reaction conditions. After a series of optimizations, we carried out a 200 mL scale-up experiment, which proved that the method has good prospects for industrial applications.

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“…In these experiments, the plasmids pGEX-4T-2 for R. salmoninarum [5], and pCG for T. fusca [36], were utilized. Other plasmids employed were the cold shock expression vector pColdI [31] and the heat inducible expression vector pCYTEXP1 [43].…”

Section: Cloning Of Glk Genesmentioning

confidence: 99%