Characterization of porcine circovirus-like virus P1 replication and lesions in BALB/c mice

Li, Wen; Gao, Xiaojing; Sheng, Shaoyang; Xiao, Qin; Wang, Wei; He, Kongwang

doi:10.1016/j.virol.2020.12.022

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…In the first week after challenge with P1 virus, the MPV of Balb/c mice significantly increased compared with that of the control group. Similarly, in the fifth week after challenge, the MCH and MCHC were significantly lower than those in the control group ( 67 ).…”

Section: Porcine Circovirus-like Virus P1 In Chinamentioning

confidence: 88%

“…Viral antigens were detected in the heart, liver, spleen, lung, testis, and thymus using immunohistochemistry. Histopathological changes of mice infected with P1 virus showed interstitial pneumonia, including perivascular connective tissue edema, mild inflammatory cell infiltration, alveolar wall thickening, myocardial necrosis, and Purkinje nucleolysis ( 67 ).…”

Section: Porcine Circovirus-like Virus P1 In Chinamentioning

confidence: 99%

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Genomic Rearrangement and Recombination of Porcine Circovirus Type 2 and Porcine Circovirus-Like Virus P1 in China

2021

Front. Vet. Sci.

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Porcine circovirus type 2 (PCV2) belongs to the genus Circovirus of the family Circoviridae, and it has been associated with porcine circovirus (associated) disease (PCVD or PCVAD) in pigs. PCVAD is the generic term for a series of disease syndromes that have caused economic losses to the pig industry worldwide. Since the discovery of PCV2 in the late 1990s, the virus has continued to evolve, and novel genotypes have continued to appear. Moreover, there has been recombination between different genotypes of PCV2. This review attempts to illustrate some progress concerning PCV2 in genome rearrangement and genomic recombination with non-PCV2-related nucleic acids, particularly focusing on the porcine circovirus-like virus P1 formed by the recombination of PCV2. The presence of rearranged PCV2 genomes can be demonstrated both in vivo and in vitro, and these subviral molecules ranged from 358 to 1,136 bp. Depending on whether it has the ability to encode a protein, the agents formed by PCV2 recombination can be divided into two categories: porcine circovirus-like viruses and porcine circovirus-like mini agents. We mainly discuss the porcine circovirus-like virus P1 regarding genomic characterization, etiology, epidemiology, and pathogenesis. Further research needs to be conducted on the pathogenicity of other porcine circovirus-like viruses and porcine circovirus-like mini agents and the effects of their interactions with PCV2, especially for the porcine circovirus-like mini agents that do not have protein-coding functions in the genome.

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Section: Porcine Circovirus-like Virus P1 In Chinamentioning

confidence: 88%

Section: Porcine Circovirus-like Virus P1 In Chinamentioning

confidence: 99%

Genomic Rearrangement and Recombination of Porcine Circovirus Type 2 and Porcine Circovirus-Like Virus P1 in China

2021

Front. Vet. Sci.

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“…The mouse model is suitable for viral pathogen studies due to its low cost, easy manipulation, and well-characterized genetic background [ 20 ]. We also investigated the host’s viral behavior and immune response [ 21 , 22 ]. Several reports have indicated that mice can serve as animal models for EHVs infection [ 12 , 19 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Characterizing the Pathogenesis and Immune Response of Equine Herpesvirus 8 Infection in Lung of Mice

Wang²,

Ren³

et al. 2022

Animals

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Equine herpesvirus type 8 (EHV-8), associated with abortion and severe respiratory disease in donkeys and horses, causes significant economic losses in the global equine industry. However, the pathogenicity of EHV-8 is still unknown. Mice are widely used as an animal model to evaluate virus replication and virulence. The present study aimed to evaluate the pathogenicity of the EHV-8 SDLC66 strain in BALB/c mice. Mice were used to test for infection-associated parameters (such as clinical signs, body weights, virus replication in tissues, viremia, and cytokines) and sacrificed at 0, 2, 4, and 6 days post-infection (dpi). The mice inoculated with EHV-8 exhibited lethargy, dyspnea signs, loss in body weight, and viremia. EHV-8 was detected in the liver, spleen, brain, and lung by PCR at 4 dpi and 6 dpi, effectively replicating these tissues detected by TCID50 at 6 dpi. Proinflammatory cytokines, including IL-6, IL-1β, and TNF-α, were significantly increased at the 4 dpi and 6 dpi in the lung than in the control group. However, IFN-γ was only increased at 6 dpi in the EHV-8-infected group. These data showed that EHV-8 could enter the lungs of mice and cause respiratory disease in the mouse model, which helps reveal the pathogenicity of EHV-8.

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“…PCV2, a member of the Circovirus genus, is the smallest, single-stranded, circular DNA virus that can replicate autonomously in mammalian cells, and is considered a primary causative agent of porcine circovirus-associated diseases (PCVADs), such as porcine post-weaning multisystemic wasting syndrome (PMWS) [ 3 ]. Current research shows that the P1 virus can naturally infect pigs, cattle, goats, rabbits, dogs, cats, and yaks, and artificially infect laboratory mice [ 1 , 4 – 7 ]. Because it was associated with PMWS, which is characterized by progressive weight loss and jaundice, the P1 virus has been linked with other emerging diseases in pigs, such as abortion and congenital tremor [ 8 – 10 ], indicating that the P1 virus may have a wider pathogenic spectrum.…”

Section: Introductionmentioning

confidence: 99%

Vertical transmission of porcine circovirus-like virus P1 in BALB/c mice

et al. 2023

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Background Porcine circovirus-like virus P1 is the animal virus with the smallest genome discovered so far, and it has become widely distributed in the Chinese mainland in recent years. Results In this study, a BALB/c mouse model was used to reveal P1 infection in female reproductive systems and the vertical transmission of the virus. The female reproductive system, including the ovary and uterus, was harvested on day 14 postinfection and examined for pathological lesions. One-day-old mice without colostrum born from infected or uninfected mothers were collected, and P1 virus distribution in the different organs was investigated. During the trials, all the mice showed no clinical symptoms or gross lesions. However, stillbirth did occur in groups infected with the P1 virus. P1 nucleic acid was detected in the heart, liver, spleen, lung, kidney, and brain tissues of 1-day-old mice born from infected mice. Microscopic lesions in P1-infected female mice were characterized by necrosis of the ovarian follicular granulosa cells and abscission, follicular atresia, necrosis of the endometrial epithelial and uterine glandular epithelial cells, and hyperplasia of the squamous endometrial epithelium. The spermatocytes in the seminiferous tubules of the infected male mice were disorderly arranged, and the germ and Sertoli cells were shed, necrotic, and decreased in number. Immunohistochemical results identified P1-positive particles in the nucleus and cytoplasm of cells from the ovary and uterus of female mice. Conclusions This study shows that the P1 virus could cause pathological damage to the reproductive system of female mice and could be transmitted vertically.

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Characterization of porcine circovirus-like virus P1 replication and lesions in BALB/c mice

Cited by 5 publications

References 17 publications

Genomic Rearrangement and Recombination of Porcine Circovirus Type 2 and Porcine Circovirus-Like Virus P1 in China

Genomic Rearrangement and Recombination of Porcine Circovirus Type 2 and Porcine Circovirus-Like Virus P1 in China

Characterizing the Pathogenesis and Immune Response of Equine Herpesvirus 8 Infection in Lung of Mice

Vertical transmission of porcine circovirus-like virus P1 in BALB/c mice

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