Characterization of primary structure and tissue expression profile of the chicken apical sodium-dependent bile acid transporter mRNA

Nakao, Nobuhiro; Kaneda, Hisako; Tsushima, Nobumichi; Ohta, Yoshiyuki; Tanaka, Minoru

doi:10.3382/ps/pev027

Cited by 10 publications

(5 citation statements)

References 27 publications

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“…In the current study, ASBT was downregulated at hatch but increased on the rest of the days post-hatch. This result agrees with previous research in which ASBT was downregulated at hatch but increased by day 7 post-hatch in chickens [ 35 ]. Additionally, other transporter genes I-BABP, OSTα, OSTβ, and BSEP were upregulated at hatch but decreased on day 3, suggesting that the reabsorption and trafficking of bile acid are age dependent.…”

Section: Discussionsupporting

confidence: 93%

The Supplementation of FloraMax-B11 Did Not Affect the Bile Acid Neosynthesis and the Enterohepatic Circulation in Broiler Chickens

Kpodo

Chaudhari

Schreier

et al. 2022

Animals

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Most probiotics possess bile salt hydrolase enzymes and may increase bile acid excretion and negatively affect fat digestion and absorption. Therefore, the study objective was to determine the time course effects of a commercial probiotic (P) FloraMax-B11 (FM) supplementation on bile acid neosynthesis and enterohepatic circulation in broiler chickens. Fertile Ross 708 eggs were incubated under standard commercial conditions. At hatch, chicks (n = 550) were randomly assigned to 5 treatment groups (n = 5 replicates per treatment group) with 22 birds per pen. The 5 treatment groups consisted of: control group (C, normal water from hatch to 35 days of age without supplements); P3, water supplemented with FM for the first 3 days post-hatch followed by normal water until day 35; P10, water supplemented with FM for the first 10 days post-hatch followed by normal water until day 35; P35, water supplemented with FM from hatch to day 35; and AGP, water supplemented with antibiotic growth promoter (AGP) from hatch until day 35. Ileum, liver, and plasma were collected at hatch, days 3, 10, 21, and 35 post-hatch. The relative mRNA expression of genes involved in bile acid synthesis (CYP7A1, CYP8B1, FXR, FGFR4, and FGF19) and transport (ASBT, I-BABP, OSTα, OSTβ, and BSEP) as well as ileal deoxycholic acid and plasma cholic acid were determined. There was no FM and AGP interaction for any of the response criteria. No FM or AGP effects were observed (p > 0.05) for any genes, except FGF19, which expression was increased (p < 0.0001) in AGP compared to P35. No FM or AGP effects were observed (p > 0.05) for levels of deoxycholic and cholic acids. However, all the genes, deoxycholic acid, and plasma cholic acid were affected by age (p < 0.0001). In general, the data indicate that FM did not negatively impact bile acid metabolism and enterohepatic circulation, which appeared to be age dependent. However, more research should be conducted to confirm these results and investigate the effects of FM on bile acid metabolism, fat digestion, and intestinal microbiota in broiler chickens.

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Section: Discussionsupporting

confidence: 93%

The Supplementation of FloraMax-B11 Did Not Affect the Bile Acid Neosynthesis and the Enterohepatic Circulation in Broiler Chickens

Kpodo

Chaudhari

Schreier

et al. 2022

Animals

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“…The microbiome in the cecum was notably different from that in the other gastrointestinal sections by PCoA score plot. The causal factors for these differences were that different gut compartments possess different functions and physiochemical characteristics ( Stanley et al., 2014 , Nakao et al., 2015 ) and that each section is inhabited by a specific microbial community ( Dethlefsen et al., 2007 , Rinttilä and Apajalahti, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Biogeography of microbiome and short-chain fatty acids in the gastrointestinal tract of duck

et al. 2020

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It is generally accepted that domestic ducks are valuable protein sources for humans. The gastrointestinal ecosystem contains enormous and complicated microbes that have a profound effect on the nutrition, immunity, health, and production of domestic ducks. To deeply understand the gastrointestinal microbial composition of domestic ducks, we investigated the microbiomes of 7 different gastrointestinal locations (proventriculus, gizzard, duodenum, jejunum, ileum, cecum, and rectum) and the short-chain fatty acids in 15 healthy muscovy ducks based on 16S rRNA gene sequencing, qPCR, and gas chromatography. As a result, 1 029 735 sequences were identified into 35 phyla and 359 genera. Firmicutes, Proteobacteria, Bacteroidetes, Cyanobacteria, and Actinobacteria were the major phyla, with Bacteroidetes being most abundant in the cecum. The population of the total bacteria and the representatives of the Firmicutes, Bacteroidetes, and Bacteroides groups increased from the proximal to the distal part of the GIT. Bacteroides was the most dominant group in the cecum. Acetate, propionate, and butytrate, as well as gene copies of butyryl-CoA including acetate-CoA transferase and butyrate kinase, were significantly higher in cecum than in other sections. Isobutyrate, valerate, and isovalerate were only found in the cecum. The differences of microbial composition and the short-chain fatty acids of their metabolites among these 7 intestinal locations might be correlated with differences in gut function. All these results provide a reference for the duck gastrointestinal microbiome and a foundation for understanding the types of bacteria that promote health and enhance growth performance and decrease instances of disease in duck breeding.

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“…Кожний відділ кишечнику демонструє відмінну функцію і фізико-хімічні характеристики, склад мікробіому (Dethlefsen et al, 2007;Rinttilä et al, 2013;Nakao et al, 2015). У товстому відділі кишечнику птахів більш дослідженими є сліпі кишки, враховуючи їх важливу роль в імунному захисті організму, а також у травленні клітковини (Pandit et al, 2018).…”

Section: вступunclassified

Microscopic structure of the rectum of the domestic duck

Makhotina¹,

Kushch²,

Bondarenko³

2020

SMLNUVMB

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As a rule, researchers pay less attention to the features of the large intestine than to the small intestine. The purpose of the study was to determine the features of the microscopic structure of the rectum of ducks during the first year of the postnatal period of ontogenesis. For histological examinations, the material was selected from Ukrainian white-breasted ducks of 9 age groups: 1-, 3-, 7-, 14-, 21-day-old, 1-, 2-, 6-month-old and 1-year-old. Morphometric parameters of microstructures were set on cross sections of the midgut. The general change pattern in the morphometric parameters of the microstructures of the rectum of ducks was their increase, which was asynchronous and uneven. There was observed an active intestinal morphogenesis continued, as evidenced by the process of formation of villi and crypts in the group of 1-3-day-old ducks. The most intensive morphometric parameters of the rectum changed in the first month of the postnatal period of ontogenesis, during which – in the first week. In the first week, first and second month of life of ducks, the wall thickness of the rectum increased by 93.1, respectively; 199.5 and 20.4 %, the thickness of the mucous membrane – 81.0; 167.1 and 19.5 %, the thickness of the muscular membrane – by 126.1; 285.3 and 22.7 %, the height of the villi – by 113.2; 208.7 and 7.2 %, the width of the villi – by 84.7; 70.9 and 12.5 %, their surface area – by 293.8; 427.6 and 20.6 %, the depth of the crypt – by 85.1; 49.9 and 52.3 %, muscle plate thickness – by 254.0; 750.8 and 15.0 %. The most stable indicators of the rectum during the first year of the postnatal period of ontogenesis were the density of villi and crypts, the number of which after 7 days of age had no significant difference compared to that of a younger age. The indicates of adult birds morphometric parameters of the rectum of ducks corresponded at different ages: in 1-year-old – the thickness of the serous membrane and the density of villi; at 6 months – the diameter of the intestine, the surface area of the villi; at 2 months of age – the thickness of the wall and its mucous membrane, the width of the villi, the depth of the crypt; at 1 month – the thickness of the muscular membrane and plate, the height of the villi, the epithelium of the villi and crypt; at 3 days of age – the density of crypts; at 1 day of age – the width of the crypt.

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Characterization of primary structure and tissue expression profile of the chicken apical sodium-dependent bile acid transporter mRNA

Cited by 10 publications

References 27 publications

The Supplementation of FloraMax-B11 Did Not Affect the Bile Acid Neosynthesis and the Enterohepatic Circulation in Broiler Chickens

The Supplementation of FloraMax-B11 Did Not Affect the Bile Acid Neosynthesis and the Enterohepatic Circulation in Broiler Chickens

Biogeography of microbiome and short-chain fatty acids in the gastrointestinal tract of duck

Microscopic structure of the rectum of the domestic duck

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