Characterization of PtAOS1 Promoter and Three Novel Interacting Proteins Responding to Drought in Poncirus trifoliata

Abstract

<jats:p>Jasmonic acid (JA) plays a crucial role in various biological processes including development, signal transduction and stress response. Allene oxide synthase (AOS) catalyzing (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT) to an unstable allene oxide is involved in the first step of JA biosynthesis. Here, we isolated the PtAOS1 gene and its promoter from trifoliate orange (Poncirus trifoliata). PtAOS1 contains a putative chloroplast targeting sequence in N-terminal and shows relative to pistachio (Pistacia vera) AOS. A number of stress-, light- and hormone-related cis-elements were found in the PtAOS1 promoter which may be responsible for the up-regulation of PtAOS1 under drought and JA treatments. Transient expression in tobacco (Nicotiana benthamiana) demonstrated that the P−532 (−532 to +1) fragment conferring drive activity was a core region in the PtAOS1 promoter. Using yeast one-hybrid, three novel proteins, PtDUF886, PtDUF1685 and PtRAP2.4, binding to P−532 were identified. The dual luciferase assay in tobacco illustrated that all three transcription factors could enhance PtAOS1 promoter activity. Genes PtDUF1685 and PtRAP2.4 shared an expression pattern which was induced significantly by drought stress. These findings should be available evidence for trifoliate orange responding to drought through JA modulation.</jats:p>

Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions

“…Sibéril et al (2001) proposed that G-BOX might involve in the transduction pathway that regulates the gene expression in the nucleus. These results suggest that genes containing the above-mentioned cis -elements might involve in different plant developmental and stress responses as reported earlier ( Wingender et al, 1990 ; Wang et al, 2011 ; Liu et al, 2016 ; Xiong et al, 2020 ). Different species such as Arabidopsis , peanut, apple, grape, rapeseed, cotton, sunflower have different numbers of chromosomes and KCS genes, suggesting that they might have gone through lineage-specific genome duplication, which is a prominent feature of plant genomes that can lead to evolution and functional novelty from existing genes ( Flagel and Wendel, 2009 ).…”

Isolation and Functional Characterization of the Promoters of Miltiradiene Synthase Genes, TwTPS27a and TwTPS27b, and Interaction Analysis with the Transcription Factor TwTGA1 from Tripterygium wilfordii

“…In silico analysis of the TwTPS27a/b promoters led to the identification of two MeJA-responsive elements, TGACG-motifs ( Figure 1 and Figure 2 A and Table S1 ), which have been reported to have a role in MeJA response [ 38 , 39 ]. Xiong et al [ 40 ] isolated the promoter of key gene PtAOS1 from Poncirus trifoliata and demonstrated that a TGACG-motif present in the core region −532 to +1 bp of PtAOS1 promoter was probably responsible for MeJA response. In this study, the 5′-deletion assays in transiently transformed tobacco plants under normal and methyl jasmonate (MeJA) conditions showed that the deletion of −921 to −391 bp in the TwTPS27b promoter caused a loss of quantitative GUS activity (−39.14% of 27bP-1 activity, Figure 2 C) and the MeJA-mediated stress induction of GUS activity was also significantly decreased (−52.06% of 27bP-1 activity, Figure 2 C).…”

Poplar Allene Oxide Synthase 1 Gene Promoter Drives Rapid and Localized Expression by Wounding

“…It is noteworthy that there are 92 hormone signaling elements responsive to gibberellin, abscisic acid, cytokinin, salicylic acid, auxin, and jasmonate in the ~2 kb OGY AOS1 promoter sequence. While induction of AOS by hormone signals remains to be demonstrated in poplar, there are reports in peach (Ibuprofen and MeJA) [18], rice (jasmonate) [14], cacao (salicylic acid, ethylene, and MeJA) [20] and trifoliate orange (MeJA and abscisic acid) [23]. The conserved motifs are largely similar among Populus AOS promoters, while there is significant difference in The phylogenetic clustering suggests that there are two types of AOS1 in the Populus genome.…”

“…Variation in AOS expression patterns among and within species, and among tissues and treatments, suggests that sequence differences in promoter regions be investigated, which might reveal novel promoter regions or motifs. The characterization of expression profiles of AOS promoters has been reported only in Arabidopsis [8][9][10], soybean [22] and trifoliate orange [23]. In the current study, we analyzed the sequence of AOS1 in Populus and investigated its promoter activity under wounding.…”