Characterization of rainbow trout, Oncorhynchus mykiss (Walbaum), serum creatine kinase isoenzymes and isoforms by means of electrophoresis and isoelectric focusing

Abstract

Serum samples were obtained from rainbow trout, Oncorhynchus mykiss (Walbaum), from a commercial fish farm. Creatine kinase (CK; EC 2.7.3.2) isoenzymes and sub-bands (isoforms) were studied using agarose gel electrophoresis and polyacrylamide gel isoelectric focusing. Creatine kinase isoenzymes and isoforms (sub-bands) were found to display disparate electrophoretic mobility and isoelectric points, respectively, when compared with CK patterns of human origin. The CK-BB isoenzyme, rarely detected in human serum, was detected in all trout samples assayed. Up to eight CKMM isoforms were detected by the polyacrylamide gel IEF method described. Correspondence

Computer-assisted evaluation of isoelectric focusing patterns in electrophoretic gels: Identification of smoothhounds(Mustelus mustelus, Mustelus asterias) and comparison with lower value shark species

“…In fact there is no significant difference between the two, both from a morphological and a commercial point of view. We selected IEF on broad pH (3.5-9.5) polyacrylamide gels, as it is a well-established and widely utilized [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18], while relatively simple, [14,19], although recently improved analytical strategies have been developed (for example, using parvalbumins as proteins of choice in fish identification [20]), performing the electrophoretic run with either CleanGels or immobilized pH gradient gels, IPGs [21]. Many less valuable shark species are sold under the generic name "smoothhound", thus committing fraudulent substitution by mislabeling the products.…”

Application of Creatine Kinase Isoenzymes for Detecting Pathophysiological Changes in Yellowtail Infected with Lactococcus garvieae.

“…Williams et al (1996) reported plasma CK isoenzyme patterns of healthy rainbow trout. It is known that the severe myopathy in "Sudden death syndrome" of Atlantic salmon was accompanied by a significant elevation of CK activity levels (Rodger et al, 1991).…”