Computer-assisted evaluation of isoelectric focusing patterns in electrophoretic gels: Identification of smoothhounds(Mustelus mustelus, Mustelus asterias) and comparison with lower value shark species

Renon, P; Colombo, Marco Maria; Colombo, Fabio; Malandra, Renato; Biondi, Pier Antonio

doi:10.1002/1522-2683(200105)22:8<1534::aid-elps1534>3.0.co;2-d

Cited by 10 publications

(6 citation statements)

References 15 publications

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“…The isoelectric focusing method used was that proposed by Righetti (1988) and used in our previous pub- lications Colombo, Colombo, Biondi, Malandra, and Renon (2000) and Renon, Colombo, Colombo, Malandra, and Biondi (2001). Briefly 2 g of muscle was placed in a plastic tube with 2 ml distilled water and homogenized with an Ultraturrax T 25 (Janke and Kunkle) at 9500 rpm for 50 s. The homogenate was centrifuged at 13,500 rpm at 4°C for 5 min (Microcentrifugette, 4214-ALC, Milan, Italy).…”

Section: Methodssupporting

confidence: 60%

Isoelectric focusing of sarcoplasmic proteins to distinguish swordfish, blue marlin and Mediterranean spearfish

Renon

Bernardi

Malandra³

et al. 2005

Food Control

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Section: Methodssupporting

confidence: 60%

Isoelectric focusing of sarcoplasmic proteins to distinguish swordfish, blue marlin and Mediterranean spearfish

Renon

Bernardi

Malandra³

et al. 2005

Food Control

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“…In this context, methods based on DNA analysis, such as RAPD-PCR, RFLP, and SSCP − or protein analysis, including SDS-PAGE, IEF , , and immunological techniques , have been developed . Furthermore, proteomics has been applied to identify fish species − and to characterize protein alterations due to postmortem changes or processing conditions .…”

Section: Introductionmentioning

confidence: 99%

Fish Authentication by MALDI-TOF Mass Spectrometry

Mazzeo

Giulio

Guerriero

et al. 2008

J. Agric. Food Chem.

143

107

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Recent EU directives and regulations for quality control and authentication of food products have prompted the development of new methods for large-scale tests to ensure the protection of consumers. In view of this, an innovative method based on MALDI-TOF mass spectrometry has been developed and successfully applied to fish authentication. Highly specific mass spectrometric profiles from 25 different fish species were obtained. Signals generated from proteins with molecular weights of about 11 kDa have been selected as specific biomarkers for unambiguous discrimination. This method is also suitable for verifying commercial product authenticity and to rapidly discriminate species subjected to fraudulent substitutions, such as those belonging to Gadidae and Pleuronectiformes. For example, biomarkers for fillets of sole (m/z 11975.21), European plaice (m/z 11351.73, 11763.63) and Greenland halibut (m/z 11432.38) were defined. Structural characterization by mass spectrometry of several proteins generating biomarker signals allowed us to identify them as parvalbumins, known to be among the major fish allergens.

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“…Several studies have been done on the composition of shark species in different regions of Mediterranean Sea (Renon et al, 2001 andIglesias et al, 2005). FAO (2005) mentioned that, 49 species of sharks belongs to 17 families and 5 orders; Hexanchiformes, Squaliformes, Squatiniformes, Lamniformes and Carcharhiniforms were recorded in the Mediterranean and Black Seas.…”

Section: Introductionmentioning

confidence: 99%

Carcharhinid shark species (Family Carcharhinidae), with special reference to the first records in the Egyptian Mediterranean Waters, Alexandria, Egypt.

Azab¹,

Khalaf-Allah²,

Sarhan³

et al. 2019

Egypt. J. of Aquatic Biolo. and Fish.

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The present study aimed to provide an update on carcharhinid sharks (Family: Carcharhinidae) in the Egyptian Mediterranean waters, based on the differences in morphological features of the body. A total of 36 specimens of carcharhinid sharks belong to 7 species of family Carcharhinidae were seasonally collected from the commercial catch at land fish market in Alexandria of Mediterranean Sea, during the period from May 2017 to June 2018. Morphometric characters were recorded for each specimen. Many photos had been captured for each shark specimen and processed by Image J software for calculating different ratios of morphological aspects for shark's body. Results showed that seven species belonging to the family Carcharhinidae (Carcharhinus altimus, C. brachyurus, C. brevipinna, C. falciformis, C. obscurus, C. plumbeus and Prionace glauca) were found to inhibit the Egyptian Mediterranean waters at Alexandria. Four of them were firstly recorded in the Egyptian Mediterranean waters (i.e. C. brachyurus, C. falciformis, C. obscurus and Prionace glauca. Our study was attempted to add some morphometric characters for the identification of studied species. Statistical analysis of morphometric ratios showed significant differences between investigated species (P<0.05), revealing its capability as classifying tool.

show abstract

Computer-assisted evaluation of isoelectric focusing patterns in electrophoretic gels: Identification of smoothhounds(Mustelus mustelus, Mustelus asterias) and comparison with lower value shark species

Cited by 10 publications

References 15 publications

Isoelectric focusing of sarcoplasmic proteins to distinguish swordfish, blue marlin and Mediterranean spearfish

Isoelectric focusing of sarcoplasmic proteins to distinguish swordfish, blue marlin and Mediterranean spearfish

Fish Authentication by MALDI-TOF Mass Spectrometry

Carcharhinid shark species (Family Carcharhinidae), with special reference to the first records in the Egyptian Mediterranean Waters, Alexandria, Egypt.

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