Characterization of reducible peptide oligomers as carriers for gene delivery

Kiselev, Anton; Egorova, Anna; Laukkanen, Antti; Baranov, V. S.; Urtti, Arto

doi:10.1016/j.ijpharm.2012.10.020

Cited by 29 publications

(57 citation statements)

References 34 publications

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“…This result indicates the susceptibility of these carriers to interaction with extracellular glycosaminoglycans. On the other hand, DNA/L0 complexes, as also shown previously, were resistant to the relaxation [21]. Slow disassembly of L0/DNA complexes may be a result of tight DNA packaging by oligoarginine.…”

Section: Discussionsupporting

confidence: 79%

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Development of a receptor-targeted gene delivery system using CXCR4 ligand-conjugated cross-linking peptides

et al. 2014

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Section: Discussionsupporting

confidence: 79%

“…Peptide buffering capacity, which is important for endosomal release, could be potentiated by substitution of lysine for histidine residues . We also found that Lys‐to‐Arg substitution results in a marked increase of transfection efficacy of cross‐linking peptides .…”

Section: Introductionmentioning

confidence: 64%

Development of a receptor-targeted gene delivery system using CXCR4 ligand-conjugated cross-linking peptides

et al. 2014

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“…Oxidative polymerization has been reported to be a useful means of polymerizing peptides which showed enhanced transfection efficiencies. 5,15 Our hypothesis was verified by the significantly higher transfection efficiency of poly-Hph-1 than PEI. Its transfection efficiency was about 10-fold higher than PEI.…”

Section: 15mentioning

confidence: 62%

“…These results show that Hph-1 binds stronger to pDNA in comparison with PEI and TAT which confirmed previous studies. 5,15 The stronger binding of DNA to Hph-1 than to TAT may be due to the aliphatic amino acid residues that allow the peptide to be relatively more flexible. Another explanation would be that because of those particular residues, Hph-1 is slightly more amphipathic than TAT 16,17 and therefore more likely to successfully deliver DNA to the cells.…”

Section: Resultsmentioning

confidence: 99%

Nonviral Gene Delivery by a Novel Protein Transduction Domain

Park

2013

Bulletin of the Korean Chemical Society

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Gene therapy using nonviral gene delivery carriers has focused on the development and modification of synthetic carriers such as liposomes and polymers. Most polymers that are commercially used are taking advantage of their polycationic character which allows not only strong ligand-DNA affinity but also competent cell penetration. Despite the relatively high transfection efficiencies, high cytotoxicity is continuously pointed out as one of the major shortcomings of polycationic polymers such as PEI. Studies on the utilization of peptides have therefore been carried out recently to overcome these problems. For these reasons, the human transcription factor Hph-1, which is currently known as a protein transduction domain (PTD), was investigated in this study to evaluate its potential as a gene delivery carrier. Although its transfection efficiency was about 10-fold lower than PEI, it displayed almost no cytotoxicity even at concentrations as high as 100 µM. Hph-1 was oxidatively polymerized to yield poly-Hph-1. The cell viability of poly-Hph-1 transfected U87MG and NIH-3T3 cells was almost as high as the control (untreated) groups, and the transfection efficiency was about 10-fold higher than PEI. This study serves as a preliminary evaluation of Hph-1 and encourages further investigation.

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“…[3,4] In recent years, non-viral vectors have been chiefly divided into two types: organic component vectors and inorganic component nanoparticles. The first type includes polymers, [5,6] cationic lipids, [7] peptides, [8,9] and carbon nanotubes [10] ; and the second type includes calcium phosphate particles, [11] quantum dots, [12] gold nanoparticles, [13] and magnetic nanoparticles. [14] Cationic polymers are one of the most significant non-viral vectors for the delivery of negatively charged DNA.…”

Section: Introductionmentioning

confidence: 99%

Diglycidyl Esters Cross-Linked with Low Molecular Weight Polyethyleneimine for Magnetofection

Feng

et al. 2015

Aust. J. Chem.

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Magnetic polyethyleneimine (PEI) complexes have demonstrated to be simple and efficient vectors for enhancing gene transfection. However, the high cytotoxicity of PEI restricts its further application in vivo. In this study, we synthesized several low cytotoxicity biodegradable cationic polymers derived from PEI (M w 600) linked with diglycidyl tartrate (DT-PEI) or its analogues (diglycidyl succinate (DS-PEI) and diglycidyl malate (DM-PEI); D-PEIs for all 3 polymers). Moreover, a type of biocompatible magnetic nanoparticles (MNPs) with negative charges was prepared to assemble with D-PEIs/DNA complexes via electrostatic interactions. The magnetic ternary complexes have appropriate sizes of 120-150 nm and zeta potential values of ,20-25 mV. The transfection ability and cell viability of D-PEIs increased as the amount of hydroxyl groups increased in the repeat unit, which indicated that increasing the hydroxyl number in the backbone of D-PEIs can enhance gene expression and decrease cytotoxicity in A549 cells. Magnetofection of DT-PEI showed similar transfection efficiency with 30 min incubation; in contrast, the standard incubation time was 4 h. All three magnetic complexes displayed lower cytotoxicity when compared with those of PEI complexes in COS-7 and A549. These results indicated that these series of magnetic PEI derivatives complexes could be potential nanocarriers for gene delivery.

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Characterization of reducible peptide oligomers as carriers for gene delivery

Cited by 29 publications

References 34 publications

Development of a receptor-targeted gene delivery system using CXCR4 ligand-conjugated cross-linking peptides

Development of a receptor-targeted gene delivery system using CXCR4 ligand-conjugated cross-linking peptides

Nonviral Gene Delivery by a Novel Protein Transduction Domain

Diglycidyl Esters Cross-Linked with Low Molecular Weight Polyethyleneimine for Magnetofection

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