Characterization of reproductive disorders in ewes given an intrauterine dose of Toxoplasma gondii tachyzoites during the intrauterine insemination

Moraes, Érica Paes Barreto Xavier de; Freitas, Amanda de Lira; Gomes-Filho, M.A.; Guerra, Maria Madalena Pessoa; Silva, M. A. R.; Pereira, MC; Braga, Valdir de Andrade; Mota, Rinaldo Aparecido

doi:10.1016/j.anireprosci.2010.07.001

Cited by 17 publications

(12 citation statements)

References 24 publications

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“…Since the distribution of T. gondii in tissues from slaughtered animals has already been studied extensively in farm animals, the present study aimed to investigate the presence of this parasite in organs that have not yet been studied, associated with reproductive aspects. The sexual transmission of this parasite was recently proven in association with the experimental infection of sheep (Moraes et al 2010a(Moraes et al , 2010b. This result inspired the performance of the present study, which aimed to detect genomic DNA of T. gondii in the uterus, tubes, ovaries, fetuses and placenta of sheep that were serologically positive and slaughtered in slaughterhouses in the state of Pernambuco, Brazil.…”

Section: Introductionmentioning

confidence: 76%

“…These results demonstrated the sexual transmission of T. gondii among sheep with a consequent vertical transm000ission to the offspring. Moraes et al (2010a and2010b) confirmed venereal transmission of the parasite during insemination with semen that had been experimentally infected with T. gondii and reported reproductive disorders including fetal death, abortion, hydrometra, mucometra, follicular cysts, anestrous and high rates of embryo resorption.…”

Section: Discussionmentioning

confidence: 95%

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Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered in abattoirs in Pernambuco, Brazil

et al. 2014

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Pesq. Vet. Bras. 34(4):329-331, abril 2014 329 RESUMO.-[Ocorrência de DNA de Toxoplasma gondii em ovelhas naturalmente infectadas e abatidas em matadouros de Pernambuco.] Objetivou-se estudar a ocorrência de anticorpos contra Toxoplasma gondii e detectar o DNA genômico do parasito em órgãos reprodutivos, fetos e anexos fetais de ovelhas em matadouros no estado de Pernambuco, Brasil. Foram coletadas amostras de soro sanguíneo, útero, trompas e ovários, além de fetos e placentas. Para a triagem utilizou-se a técnica de Imunofluorescência Indireta (RIFI) e para a detecção do DNA de T. gondii empregou-se a Reação em Cadeia da Polimerase (PCR) nos animais positivos na sorologia e em todos os fetos e anexos fetais. Na sorologia, 13/50 amostras foram positivas e o DNA genômico de T. gondii foi detectado em uma amostra de útero, trompa, ovário, placenta e feto (coração, cérebro e cordão umbilical) de uma ovelha positiva na sorologia. A identidade molecular dos produtos amplificados foi confirmada por sequenciamento. Neste estudo comprova-se a ocorrência do DNA de T. gondii em órgãos do sistema reprodutivo, placenta e feto de ovelha naturalmente infectada. TERMOS DE INDEXAÇÃO: Toxoplasmose, Toxoplasma gondii, ovelha, Reação em Cadeia da Polimerase.

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Section: Introductionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 95%

Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered in abattoirs in Pernambuco, Brazil

et al. 2014

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“…Its tachyzoites were detected in testis and epididymis of experimentally infected intermediate hosts using immunohistochemistry test ). Infection to the parasite has also been reported in ewes after use of contaminated semen in artificial insemination (Moraes et al 2010).…”

Section: Discussionmentioning

confidence: 98%

“…The presence of the parasite has also been confirmed by bioassays, immunohistochemistry and PCR methods in semen of male dogs and rams experimentally infected with T. gondii (Vyas 2013). Moreover, it was shown that artificial insemination with contaminated semen could lead to toxoplasmosis in ewes (Moraes et al 2010). …”

Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii: sexual transmission in mice

et al. 2013

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This study was performed to evaluate sexual transmission of Toxoplasma gondii in mice. RH strain tachyzoites were intraperitoneally inoculated into 10 Balb/C male mice and after 48 h, their semen were collected from epididymis and examined by giemsa staining and PCR. Twenty Balb/C female mice mated with four infected male mice four times and any mating time was 48 h whilst 20 female control mice mated with four uninfected male mice for 8 days. Female mate choice was assessed using a threechambered cage. Four female mice were placed in a central chamber and in one side of it, two infected male mice were kept and in other side, two naïve male mice were placed. Due each quarter, every of the female movement was reported and then the female was replaced to middle chamber. Besides on the detection of DNA and whole parasite in semen, no abortion and death was seen in female mice. Pregnancy was seen only 4 out of 20 female mice which mated with infected males while 17 pregnancies were seen from 20 control female mice (P value = 0.0001). No statistical significant was seen in female mate choice between naïve male (45 movement) and infected male (36 movement). This study showed that toxoplasmosis could not transmit to female mice and their offspring due to mating and the parasite had not effect on female mate choice. It seems that infected male mice cannot entirely mate with females due to reduction of male weapon and body size, physiological vigor and energy.

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“…A busca do parasito em órgãos reprodutivos de carneiros naturalmente infectados realizado neste estudo foi estimulada, em parte, pelos resultados obtidos na infecção experimental realizada por Moraes et al (2010b) e Moraes et al (2010c) que infectaram ovelhas com sêmen contaminado com diferentes doses de T. gondii e observaram, através de exames ultrassonográficos, transtornos reprodutivos como reabsorções embrionárias, morte fetal e aborto. O estudo comprovou a transmissão do parasito via sêmen experimentalmente contaminado em ovelhas e uma elevada frequência de absorção embrionária, indicando a colonização precoce da placenta e embrião no início da gestação, o que demonstrou que este aspecto deveria ser melhor investigado na infecção natural.…”

Section: Discussionunclassified

Detecção de Toxoplasma gondii em órgãos do sistema reprodutivo de carneiros naturalmente infectados no Brasil

et al. 2013

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Characterization of reproductive disorders in ewes given an intrauterine dose of Toxoplasma gondii tachyzoites during the intrauterine insemination

Cited by 17 publications

References 24 publications

Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered in abattoirs in Pernambuco, Brazil

Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered in abattoirs in Pernambuco, Brazil

Toxoplasma gondii: sexual transmission in mice

Detecção de Toxoplasma gondii em órgãos do sistema reprodutivo de carneiros naturalmente infectados no Brasil

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