Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered in abattoirs in Pernambuco, Brazil

Bezerra, Mauro José Gonçalves; Cruz, J.A.L.O.; Kung, Eugênio Souza; Silva, José Givanildo da; Santos, André de Souza; Moraes, Érica Paes Barreto Xavier de; Pinheiro, José Wilton

doi:10.1590/s0100-736x2014000400005

Cited by 11 publications

(3 citation statements)

References 19 publications

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“…Anestrus, follicular cysts, and salpingitis/hydrosalpinx were detected in the chronic phase of infection. Although the results of the present study were not able to confirm the presence of T. gondii in the reproductive tract (using either PCR or histopathological tests), T. gondii DNA was detected by PCR in the uterus, ovaries and tubes of a naturally infected sheep (BEZERRA et al, 2014). Moreover, reproductive failure due to T. gondii was previously described by STAHL et al (1994) among chronically infected female mice (cessation of estrous and constant diestrus cytology after one month), associated with marked infertility and uterine atrophy two months after infection, as well as impaired folliculogenesis.…”

Section: Discussioncontrasting

confidence: 90%

Distúrbios reprodutivos em cabras experimentalmente infectadas por Toxoplasma gondii

Wanderley

Porto

Câmara

et al. 2019

Revista Brasileira De Ciência Veterinária

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Objetivou-se descrever os distúrbios reprodutivos associados à infecção experimental por Toxoplasma gondii através da inseminação artificial com sêmen contaminado em quatro cabras no estágio crônico da infecção. As características do trato reprodutor foram avaliadas através de ultrassonografia transretal, visando o diagnóstico gestacional ou de desordens reprodutivas, após a infecção experimental. Ao final do experimento, os animais foram necropsiados e avaliações histopatológicas e PCR foram realizados. Dentre os animais infectados que exibiram mortalidade embrionária, duas apresentaram anestro e duas apresentaram repetição de estro, sendo que destas uma apresentou intervalos entre estros reduzido (sete dias) e outra em intervalo regular (21 dias). Todavia, ambas foram submetidas a monta natural durante os estros naturais subsequentes e não foi confirmada gestação até o final do experimento (90 dias). Duas cabras exibiram alterações nos exames de ultrassonografia, sendo identificadas um cisto ovariano, e uma hidrossalpinge, ambas confirmadas no exame post-mortem. As principais lesões microscópicas nesse grupo foram infiltração neutrofílica dos pulmões, glomerulonefrite intersticial e infiltração neutrofílica do fígado. O DNA de T. gondii foi encontrado nos órgãos (coração e cérebro) de três cabras. Em conclusão, cabras infectadas com sêmen contendo T. gondii no momento da inseminação artificial apresentam distúrbios reprodutivos na fase crônica da infecção que podem estar associados à toxoplasmose.

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Section: Discussioncontrasting

confidence: 90%

Distúrbios reprodutivos em cabras experimentalmente infectadas por Toxoplasma gondii

Wanderley

Porto

Câmara

et al. 2019

Revista Brasileira De Ciência Veterinária

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“…A total of 57 peer-reviewed published articles studying the prevalence of toxoplasmosis in animals slaughtered in Brazilian abattoirs were found ( Table S2 ) [ 9 , 10 , 11 , 12 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ,…”

Section: Resultsmentioning

confidence: 99%

A Systematic Review and Meta-Analysis on the Occurrence of Toxoplasmosis in Animals Slaughtered in Brazilian Abattoirs

Rossi

Costa

Gabriël

et al. 2022

Animals

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Toxoplasmosis is mainly transmitted to human beings through the consumption of contaminated food, and several outbreaks caused by infected meat consumption have been reported in Brazil. We performed a systematic literature review on the prevalence and risk factors for toxoplasmosis in slaughtered animals and performed a meta-analysis of its prevalence for different species and regions. Furthermore, we also discussed the infectivity of seropositive animals, risk factors, and preventive strategies. In the meta-analysis, the overall prevalence estimates for poultry, ostrich, goats, swine, equids, sheep, and bovines were 42.4, 40.4, 23.0, 19.9, 19.1, 17.3, and 16.2%, respectively. Regarding the Brazilian regions, the highest prevalence values were detected for bovines and equids in the South (32.3 and 34.4%, respectively) and swine, goats, sheep, and poultry in the Northeast (29.3, 23.0, 22.9, and 69.8%%, respectively). High proportions of Toxoplasma gondii viability in bioassay conducted on seropositive animals were seen for sheep (34/40 = 85%) and swine (12/15 = 80%). Toxoplasma gondii infections are widespread on Brazilian farms, and the summarized data allow the establishment of high-priority areas and/or species for the adoption of preventive strategies to control this parasite at different levels of the food chain.

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“…In Kerala, a total of 98 goats screened for the presence of toxoplasma antibodies, and 58 were found positive [ 39 ]. Among 13 seropositive goats, the uterus of one goat showed the presence of the parasite DNA by PCR in Brazil [ 40 ]. In the present study, all samples were negative for T. gondii by PCR.…”

Section: Discussionmentioning

confidence: 99%

Molecular etiopathology of naturally occurring reproductive diseases in female goats

Beena¹,

Rvs²,

Kumaresan³

et al. 2017

Vet World

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Aim:The aim of the present study was to investigate the molecular etiopathology of occurrence of reproductive diseases in female goats. Reproductive diseases in goats account for major economic losses to goat farmers in terms of valuable loss of offspring and animal productivity.Materials and Methods:A total of 660 female genitalia were examined for pathological conditions (macroscopic and microscopic lesions). The etiopathological study was carried out for the presence of pathogenic organisms such as Brucella, Chlamydia, and Campylobacter in the uterus and ovary. Based on the microscopic lesions, suspected samples were subjected to diagnostic polymerase chain reaction (PCR) for various etiological agents employing 16srRNA genus specific primers for Campylobacter and Chlamydophila and OMP31 gene-based PCR for Brucella melitensis and nested PCR using ITS-1 gene primers for Toxoplasma gondii. For Brucella suspected samples, immunohistochemistry (IHC) was also performed.Results:In studied female genitalia, 108 (16.30%) showed gross abnormalities with overall 23.32% occurrence of pathological conditions (macroscopic and microscopic lesions). Pathological involvement of the uterus was the highest 68 (62.96%), followed by the ovaries 27 (25%) and other organs. Major uterine condition observed was endometritis (5.60%). In uterine infections, 35 (5.30%) samples were found positive for Campylobacter spp., 12 (1.81%) samples for B. melitensis, and 3 (0.45%) samples were positive for Chlamydophila spp. Among the samples positive for B. melitensis by PCR, 3 were found positive by IHC also. Corynebacterium ovis was detected by PCR using specific primers in a case of hydrosalpinx. It was concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats.Conclusion:The present study concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats.

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Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered in abattoirs in Pernambuco, Brazil

Cited by 11 publications

References 19 publications

Distúrbios reprodutivos em cabras experimentalmente infectadas por Toxoplasma gondii

Distúrbios reprodutivos em cabras experimentalmente infectadas por Toxoplasma gondii

A Systematic Review and Meta-Analysis on the Occurrence of Toxoplasmosis in Animals Slaughtered in Brazilian Abattoirs

Molecular etiopathology of naturally occurring reproductive diseases in female goats

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