Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. nov.

Wei, Ge Hong; Tan, Zhi Yuan; Zhu, Ming; Wang, En Tao; Han, Su Zhen; Chen, Wen Xin

doi:10.1099/ijs.0.02031-0

Cited by 75 publications

(40 citation statements)

References 33 publications

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“…Chickpea rhizobia was reported to have both fast and slow growing strains (Nour et al, 1994). Moreover, isolates obtained from chickpea nodules failed to grow on BTB-medium (Wei et al, 2003). Most of the isolates were Congo red dyes absorbent, except the three DMU-1, DMU-2 and DMU-3.…”

Section: Resultsmentioning

confidence: 99%

Phenotypic characterization and symbiotic effectiveness test of chickpea (Cicer arietinum L.) rhizobia isolated from Dejen and Aneded Districts, East Gojjam Zone, Amahara Region, Ethiopia

Aynalem¹,

Tadele²,

Bezie³

et al. 2018

Afr. J. Biotechnol.

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Chickpea (Cicer arietinum L.) is an important leguminous crop grown in different parts of Ethiopia.It is a nutritionally valued and N 2 -fixing legume which forms a symbiotic association with Mesorhizobium. This study was conducted to characterize and evaluate symbiotic efficacy of chickpea rhizobia isolated from soil samples collected from the study area. Seventeen chickpea rhizobia were isolated by soil host plant trap method and characterized for edaphic stress tolerance. Five of the isolates were grown at high temperature of 45°C and salt concentration of 6%, whereas seven were grown at acidic pH of 4, and four were grown at basic pH of 9. All isolates were not resistant to kanamycin, while fair resistance to erythromycin and streptomycin and modest resistance to ampicillin and azithromycin were observed. Furthermore, most of the isolates showed a variation in nodulation with higher (22 NN/p) and lowest (9 NN/p) scores. Shoot dry weight (SDW) of the plant ranged from 1.18 to 1.84 g/p and isolates showed effective (67%) to highly effective (100%) N 2 -fixing performance. From these, four isolates showed multiple edaphic stress resistance and are recognized as promising candidate for chickpea production in stressed soil; however, further study in the filed is required.

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Section: Resultsmentioning

confidence: 99%

Phenotypic characterization and symbiotic effectiveness test of chickpea (Cicer arietinum L.) rhizobia isolated from Dejen and Aneded Districts, East Gojjam Zone, Amahara Region, Ethiopia

Aynalem¹,

Tadele²,

Bezie³

et al. 2018

Afr. J. Biotechnol.

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“…Rhizobium species are traditionally considered as legume endosymbionts and have generally been isolated from nodules on leguminous plants (Amarger et al, 1997;de Lajudie et al, 1998;Lindström, 1989;Squartini et al, 2002;Valverde et al, 2006;van Berkum et al, 1998;Wang et al, 1998;Wei et al, 2002Wei et al, , 2003, with the exception of Rhizobium selenitireducens and Rhizobium daejeonense, isolated from bioreactors (Hunter et al, 2007;Quan et al, 2005), and Rhizobium cellulosilyticum, from sawdust of Populus alba (García-Fraile et al, 2007). Large populations of rhizobia are found both in the bulk soil and in the rhizospheres of legumes and other plants (Schloter et al, 1997;Segovia et al, 1991;Sullivan et al, 1996).…”

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confidence: 99%

Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta

Peng

Yuan

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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During a study of endophytic nitrogen-fixing bacteria present in the wild rice species Oryza alta, eight novel isolates were obtained from surface-sterilized roots and classified in the genus Rhizobium on the basis of almost-complete 16S rRNA gene sequence analysis. These strains can nodulate Phaseolus vulgaris and Glycine max. The highly similar protein patterns, DNA fingerprint patterns of insertion sequence-based PCR (IS-PCR) and DNA-DNA hybridizations showed that these novel isolates were members of the same species. The closest phylogenetic relatives of the representative strain Alt 505T of the novel group were Rhizobium etli CFN 42 T and Rhizobium indigoferae CCBAU 71714 T , with 96.2 and 96.0 % 16S rRNA gene sequence similarity, respectively. Low DNA-DNA relatedness with the type strains of R. etli, R. indigoferae, Rhizobium hainanense, Rhizobium mongolense and Rhizobium galegae and differences in IS-PCR fingerprinting patterns, SDS-PAGE of proteins, antibiotic resistance, phenotypic tests and comparison of cellular fatty acids with Rhizobium species indicated that the novel group of isolates were distinct from previously described species. Based on these results, we propose to place them in a novel species, as Rhizobium oryzae sp. nov. The type strain is Alt 505 T (5LMG 24253 T 5CGMCC 1.7048 T ).

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“…Several novel rhizobial taxa have been described from this region: the genus Sinorhizobium (Chen et al, 1988) and species Mesorhizobium tianshanense (Chen et al, 1995), Mesorhizobium amorphae (Wang et al, 1999), Sinorhizobium xinjiangense (Chen et al, 1988;Peng et al, 2002), Sinorhizobium kummerowiae (Wei et al, 2003), Rhizobium yanglingense (Tan et al, 2001), Rhizobium indigoferae (Wei et al, 2002) and Rhizobium loessense (Wei et al, 2003). In this paper we describe some isolates from the legume Alhagi sparsifolia, which grows wild in this region.…”

mentioning

confidence: 99%

Mesorhizobium alhagi sp. nov., isolated from wild Alhagi sparsifolia in north-western China

Chen

Zhu

Bontemps

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Eleven strains that formed symbiotic root nodules on Alhagi sparsifolia, designated previously as genospecies II, were identified as a new lineage of Mesorhizobium (Alphaproteobacteria) that could be differentiated from all previously recognized species of the genus Mesorhizobium by using 16S rRNA gene sequences (<97.8 % similarity), DNA–DNA hybridization (<45 %), dnaJ, dnaK, recA, glnA, nifH, nodA and nodC gene sequences, fatty acid profiles (C18 : 1 ω7c, 35 %;11-methyl C18 : 1 ω7c, 30 %) and numerical taxonomy. These strains are therefore considered to represent a novel species, for which the name Mesorhizobium alhagi sp. nov. is proposed, with isolate CCNWXJ12-2T (=ACCC 15461T=HAMBI 3019T) as the type strain.

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Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. nov.

Cited by 75 publications

References 33 publications

Phenotypic characterization and symbiotic effectiveness test of chickpea (Cicer arietinum L.) rhizobia isolated from Dejen and Aneded Districts, East Gojjam Zone, Amahara Region, Ethiopia

Phenotypic characterization and symbiotic effectiveness test of chickpea (Cicer arietinum L.) rhizobia isolated from Dejen and Aneded Districts, East Gojjam Zone, Amahara Region, Ethiopia

Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta

Mesorhizobium alhagi sp. nov., isolated from wild Alhagi sparsifolia in north-western China

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