Characterization of RTG-2 Fish Cell Line by Random Amplified Polymorphic DNA

Ferrero, Mar; Castaño, Argelia; González, Ana; Sanz, Félix; Becerril, Concepción

doi:10.1006/eesa.1998.1642

Cited by 19 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A set of 10 OPA oligonucleotide random (10-mer primers) were purchased from Operon Technologies. The RAPD protocol was carried out as described by [10]. Briefly, the RAPD reaction was performed with 100 ng DNA template in a total volume of 25 lL containing 2.5 lL of 10· enzyme assay buffer, 100 lM each of dATP, dCTP, dGTP, and dTTP, 2 nM of random (10 bp) primer, 1.5 mM MgCl 2 , and 1.5 U of Taq DNA polymerase (Jena Bioscience GmbH-Lobstedter Str.…”

Section: Dna Extraction and Rapd Analysismentioning

confidence: 99%

Genotoxicity assessment of ammonia in cultured Oreochromis niloticus using RAPD assay

Abumourad¹,

Hanna

Girgis

2012

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Limited efforts have been made to study the genotoxic effects of ammonia in cultured OOreochromi niloticus. Therefore, the present study was planned to assess the genotoxic effect of ammonia in cultured O. niloticus using random amplified polymorphic DNA (RAPD) assay. Fish was categorized into four groups. The 1st group exposed to 2.5 mg/L of total ammonia nitrogen (TAN) (0.16 NH 3 mg/L), the 2nd exposed to 5.0 mg/L of total ammonia nitrogen (TAN) (0.32 NH 3 mg/L) and the 3rd exposed to 10.0 mg/L of total ammonia nitrogen (TAN) (0.65 NH 3 mg/ L), in addition to control group for the treatment period of 6 days. The results revealed that some genes in O. niloticus are susceptible to DNA disturbances/mutation as a result of exposure to high concentration of ammonia in water, this clearly indicated using RAPD screening assay.

show abstract

Section: Dna Extraction and Rapd Analysismentioning

confidence: 99%

Genotoxicity assessment of ammonia in cultured Oreochromis niloticus using RAPD assay

Abumourad¹,

Hanna

Girgis

2012

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Studies concerning fish developmental biology (Ma et al 2001), toxicology (Ferrero et al 1998), physiology (Hightower and Renfro 1988) and molecular genetics (Alvarez et al 1991;Hong et al 1998) and transgenic applications have incorporated the use of cell lines. Cell lines have been developed from various commercially important fish species and have been applied as experimental models for assessment of heavy metal toxicity (Ryan and Hightower 1994) and for propagation and studies on fish viruses Chi et al 1999;Kang et al 2003;Lai et al 2000Lai et al , 2001Lai et al , 2003Lannan et al 1984).…”

Section: Introductionmentioning

confidence: 99%

Development of a black sea bream fibroblast cell line and its potential use as an in vitro model for stress protein studies

Zhou

Deane

Woo

2003

Fish Physiol Biochem

View full text Add to dashboard Cite

A fibroblast cell line (BSF) derived from a caudal fin explant of black sea bream (Mylio macrocephalus) was developed. The optimum fetal bovine serum (FBS) concentration for fibroblast cell line growth was found to be 15-20% v/v FBS and the optimum temperature range for growth was found to be 26-30 • C. The fibroblast cells displayed a diverse distribution in chromosome number with two modal chromosome numbers of 48 and 54. Upon acute heat shock (+8 • C) the cells displayed a 4.1 fold increase in hsp70 and this elevation was not prolonged as hsp70 returned to near basal levels following a 6 h recovery period. The effect of the hsp70 inducer L-azetidine-2-carboxylic acid was tested and it was found that at a concentration of 10 mM this inducer caused a 2.3 fold increase in hsp70 levels. The sensitivity of the fibroblast cell line to heavy metal exposure was tested by treatment with Cu 2+ and it was found that hsp70 was significantly elevated in the presence of micromolar concentrations of Cu 2+ . The data from this study demonstrates that the established black sea bream fibroblast cell line could serve as a useful in vitro model for stress protein studies.

show abstract

“…For example, a rainbow trout (Oncorhynchus mykiss) fibroblastic cell line, RTG-2, has been applied to assess chemical-caused genotoxicity using random amplified polymorphic DNA (RAPDs) analysis [35] or micronuclei (MN) estimations by means of flow cytometry [36]. An embryonic fibroblast-derived cell line XTC-2, which was derived from the African clawed frog (Xenopus laevis), was used to investigate exposure to environmentally relevant concentrations of the bactericidal agent triclosan by quantifying effects on thyroid hormone associated gene expression [37].…”

Section: Cell-based In Vitro Bioassaysmentioning

confidence: 99%

Toxicology of Water

Zhang

Wiseman

Giesy

2012

Experientia Supplementum

View full text Add to dashboard Cite

To protect the quality of water from toxic pollutants for the health of humans and the environment, two approaches are generally applied in the field of toxicology to predict the effects of pollutants and to monitor the toxic pollutants in water. Here we provide our perspective on state-of-the-art methods to develop water quality criteria and the use of molecular techniques for monitoring water quality. Emphasized is the recent development and application of cell-based assays and small fish model in toxicology research of water.

show abstract

Characterization of RTG-2 Fish Cell Line by Random Amplified Polymorphic DNA

Cited by 19 publications

References 23 publications

Genotoxicity assessment of ammonia in cultured Oreochromis niloticus using RAPD assay

Genotoxicity assessment of ammonia in cultured Oreochromis niloticus using RAPD assay

Development of a black sea bream fibroblast cell line and its potential use as an in vitro model for stress protein studies

Toxicology of Water

Contact Info

Product

Resources

About