Characterization of Salinity-Tolerant Mutant of Anabaena doliolum Exhibiting Multiple Stress Tolerance

Singh, Devendra P.; Kshatriya, K.

doi:10.1007/s00284-001-0112-7

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…In both plants and cyanobacteria, similar effects on chlorophyll content and carotenoids observed were, namely, reduction in chlorophyll contents in salt-sensitive (Singh and Kshatriya 2002;Srivastava et al 2005) and increase in salt-tolerant species (Saleh 2012;Lu and Vonshak 1999). Thus, chlorophyll content could be used as a parameter for selection of tolerant varieties of crop plants (Eryilmaz 2007).…”

Section: Photosynthesismentioning

confidence: 57%

Signal Perception and Mechanism of Salt Toxicity/Tolerance in Photosynthetic Organisms: Cyanobacteria to Plants

Agrawal

Sen

Chatterjee

et al. 2015

Stress Responses in Plants

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High salt concentration represents one of the most significant abiotic constraints, affecting all life forms including plants and cyanobacteria. Soil salinity curtails plant growth by way of osmotic, ionic and oxidative stresses resulting in multiple inhibitory effects on various physiological processes such as growth, photosynthesis, respiration and cellular metabolism. In order to combat high salinity, various adaptive strategies employed include ion homeostasis achieved by ion transport and compartmentalization of injurious ions, osmotic homeostasis by accumulation of compatible solutes/osmolytes and upregulation of antioxidant defence mechanism. The aforesaid processes are executed through SOS and MAPK signalling pathways leading to modulation of gene expression. Salt stress signal transduction pathways initiate through sensing extracellular Na + ions causing modification of constitutively expressed transcription factors. This modification is responsible for expression of early transcriptional activators such as CBF/DREB gene family which eventually activate stress tolerance effector genes such as osmolyte biosynthesis genes, detoxification enzymes, and chaperones. Various genes/cDNAs encoding proteins involved in these adaptive mechanisms have been isolated and identified. Bioinformatic predictions through docking revealed interaction of salt across the species at conserved domains and motifs as a possible mechanism for response of a particular protein under salt stress. In this chapter, major aspects of salt stress are reviewed with emphasis on its detrimental consequences and biochemical and molecular mechanisms of signal transduction in plants and cyanobacteria under high salinity.

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Section: Photosynthesismentioning

confidence: 57%

Signal Perception and Mechanism of Salt Toxicity/Tolerance in Photosynthetic Organisms: Cyanobacteria to Plants

Agrawal

Sen

Chatterjee

et al. 2015

Stress Responses in Plants

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“…Random mutagenesis is another tool used for generating mutants of different microorganisms. Different mutation techniques, that is, chemical (N-methyl-Ń-Nitro-nitrosoguanidine, NTG) and physical mutagenesis (atmospheric and room temperature plasmas, ARTP) have been applied to A. platensis to generate mutants with striking properties [5,21,22]. Keeping these facts in view, present communication deals with the isolation and characterization of mutants of A. platensis with high protein and phycocyanin contents.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of high protein and phycocyanin producing mutants ofArthrospira platensis

et al. 2017

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Cyanobacteria are known to exhibit their efficiency in producing high concentrations of compounds of commercial value. Arthrospira is one such cyanobacterium which is considered as important source of protein (65%) and other nutrients. In present study, chemical mutagenesis using N-methyl-Ń-Nitro-nitrosoguanidine (NTG), a proven potent mutagen for cyanobacteria was used to bring stable and desirable alteration in Arthrospira platensis ARM 730. Three morphological mutants (G-1, G-2, and SF) were selected and characterized. The G-1 and G-2 were helical, more bluish in pigmentation than the wild type strain where G-1 also showed enlarged cell size. The SF mutant was an altered straight-filament having maximum biomass. Among three mutants, higher protein and phycocyanin contents were observed in G-1 and G-2 mutants whereas chlorophyll was less in these mutants as compared to wild type strain indicating change in the pigment ratio. Carotenoid content was higher in SF mutant as compared to wild type and other mutants. Variation in total sugar content was not observed in comparison to wild type strain. The analysis of amino acid spectrum of all the mutants and wild type showed significant increase in proline content. Overall, it is revealed from the results that G-1 and G-2 mutants showed higher biomass, phycocyanin, and protein contents in comparison to wild type which indicated their great potential to be used in food and pharmaceutical industries.

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“…Microcystis might bloom up to 12.5-14 g l -1 (Verspagen et al 2006a;Tonk et al 2007) or 25 g l -1 (Robson and Hamilton 2003). Anabaena reduces its growth rate to 30 % at 9 or 15 g l -1 (Singh and Kshatriya 2002). Here this option is not explored; increasing up to these ranges will have direct and drastic effects on all aquatic biota, may induce increased sediment phosphorus release (Barker et al 2008), and may introduce marine noxious species such as Nodularia (Rakko and Seppälä 2014).…”

Section: Chemicalmentioning

confidence: 99%

How to combat cyanobacterial blooms: strategy toward preventive lake restoration and reactive control measures

Stroom

Kardinaal

2016

Aquat Ecol

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Water managers worldwide are facing the serious problem of dense blooms of cyanobacteria in surface waters. In the quest for the optimal method to combat cyanobacterial dominance, many questions and many possible solutions arise. This paper presents a three-phase strategy to fight harmful cyanobacterial proliferation. Water managers who apply this strategy can generate a tailor-made set of measures. The three phases consist of (1) defining the ecological water quality targets of the waterbody in question, (2) assessing its current ecological state, and (3) selecting which measures will yield optimal results. The paper provides assistance in the quantitative diagnosis of the state of both shallow and deep temperate freshwater lakes by means of a lake system analysis, and presents a survey of measures. Measures are divided into two sets. Preventive measures are based on switching to a clear state (shallow lake) or reducing the overall cyanobacterial biomass (deep lake). They are subdivided into nutrient reduction, hydromorphological adjustments, and food web management. Concerning nutrient reduction, in many situations phosphorus management alone should suffice. Nitrogen management can be important to increase the species diversity of macrophytes, or to relieve downstream (marine) consequences. Control measures (including mitigation) have a direct impact on cyanobacterial blooms by biomass removal, flushing, or mixing; however, the number of proven technologies is limited.

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Characterization of Salinity-Tolerant Mutant of Anabaena doliolum Exhibiting Multiple Stress Tolerance

Cited by 8 publications

References 18 publications

Signal Perception and Mechanism of Salt Toxicity/Tolerance in Photosynthetic Organisms: Cyanobacteria to Plants

Signal Perception and Mechanism of Salt Toxicity/Tolerance in Photosynthetic Organisms: Cyanobacteria to Plants

Isolation and characterization of high protein and phycocyanin producing mutants ofArthrospira platensis

How to combat cyanobacterial blooms: strategy toward preventive lake restoration and reactive control measures

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