Characterization of Salmonella enterica serovar Typhimurium isolates associated with septicaemia in swine

Abstract

In th1s study we characterized, usmg genotyping and phenotypmg methods, ISolates {n=33) from septicaemia outbreaks in swine herds as well as isolates {n=33) recovered from healthy ammals at slaughter. We determined the antimicrobial agents resistance profiles using 24 different an timicrobial agents by the d1sk d1ffus1on on agar method, the phage type, the plasmid profiles and the PFGE profiles usmg Xbal and Spel as restriction enzymes for each isolates. Resistance to as much as 10 antimicrobial agents was found in both categories of isolates. A greater number of PFGE genotypes was observed in isolates from septicaemia. Various phage types were 1dent1fied in both groups of isolates. Among the DT1 04 phage type, many genetic clusters were 1dent1fied Analysis of plasmid profiles indicated that septicemic strains possess higher molecular we1ght plasmids than asymptomatic 1solates. These results Indicated that strams associated w1th sept1caem1a belong to various genet1c lineages and suggest that VIrulence tra1ts are assoc1ated w1th plasmid profiles of stra1ns. Our results also suggest that the genetic d1vers1ty of Salmonella DT 104 m1ght be higher m North Amenca if we cons1der results of s1milar studies m Europe

Avaliação clínica da infecção experimental de bezerros com Salmonella Typhimurium

“…A diarreia neonatal de bezerros acarreta graves prejuízos à pecuária tanto pela mortalidade quanto pelos custos envolvidos com o tratamento e atraso no crescimento dos animais, sendo a salmonelose uma das causas mais importantes, com destaque para Salmonella (S.) enterica subespécie enterica sorotipos Typhimurium e Dublin (Fecteau et al, 2003). A principal porta de entrada para a ocorrência da infecção é a cavidade oral, pela ingestão de água e alimentos contaminados.…”

“…A infecção resulta em ampla variedade de sinais clínicos, em função da idade do animal e do sorotipo envolvido, sendo necessárias doses de 10 9 e 10 10 unidades formadoras de colônia (UFC) de S. Typhimurium para provocar a doença em bovinos sadios (Santos et al, 2002). Em bezerros com até oito semanas de idade, a infecção pode causar diarreia aguda, aquosa e fétida, desidratação, desequilíbrio eletrolítico, endotoxemia, bacteremia e morte (Fecteau et al, 2003), sendo a taxa de mortalidade inversamente proporcional à idade (Santos et al, 2002).  Os objetivos do presente estudo foram avaliar o quadro clínico de bezerros submetidos à infecção experimental com 10 9 UFC de S. Typhimurium e verificar a viabilidade do modelo experimental na indução da doença em bezerros.…”

“…Antes da inoculação com S. Typhimurium, todos os parâmetros clínicos estavam dentro dos valores considerados normais para a espécie bovina (Feitosa, 2008). Entre 24 e 72 horas após a infecção experimental, todos os animais inoculados com 10 9 UFC de S. Typhimurium apresentaram diferentes graus de diarreia (Tab.…”

An Investigation into the Efficacy of Washing Trucks Following the Transportation of Pigs—ASalmonellaPerspective

“…In comparison, Lukinmaa et al (2006) found that 96% of multiresistant DT104 strains studied belonged to one particular PFGE type. Interestingly, results from a study by Bergeron et al (2007) suggest a greater genetic diversity of Salmonella Typhimurium DT104 in North America than that in Europe. Despite the limitations of PFGE analysis in the current study, it is an invaluable tool for the epidemiological typing of Salmonella serovars; however, it may be inaccurate to draw epidemiological conclusions based on a single fingerprinting method (Liebana et al, 2002).…”

Expression of flak flagellin from Salmonella Typhimurium in tobacco seeds

“…This status establishes the maintenance of the infection in the involved farms, causing eminent economic losses for intensive livestock farming, and represents an important public health risk [4]. Swine represent reservoir for human infections [5]. In fact according to scientific report of EFSA the prevalence of Salmonella-positive slaughter pigs was up to 10.3%, although with variation among Member States, including both primary infections and surface contamination of carcasses.…”