Expression of flak flagellin from Salmonella Typhimurium in tobacco seeds

Rossi, Luciana

doi:10.9790/3013-25201922

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Different lines were compared for transgene transcription, and lines with stronger signals were selected for subsequent plant generations [22]. Overall, 14 ± 1 mg of soluble proteins were extracted from samples of 100 mg of tobacco seeds.…”

Section: Resultsmentioning

confidence: 99%

Expression of verocytotoxicEscherichia coliantigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

et al. 2013

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Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral administration in transgenic seeds could be an efficient system to stimulate local immunity. This study was conducted to transform tobacco plants for the seed-specific expression of antigenic proteins from a porcine verocytotoxic E. coli strain. Parameters related to an immunological response and possible adverse effects on the oral administration of obtained tobacco seeds were evaluated in a mouse model. Tobacco was transformed via Agrobacteium tumefaciens with chimeric constructs containing structural parts of the major subunit FedA of the F18 adhesive fimbriae and VT2e B-subunit genes under control of a seed specific GLOB promoter. We showed that the foreign Vt2e-B and F18 genes were stably accumulated in storage tissue by the immunostaining method. In addition, Balb-C mice receiving transgenic tobacco seeds via the oral route showed a significant increase in IgA-positive plasma cell presence in tunica propria when compared to the control group with no observed adverse effects. Our findings encourage future studies focusing on swine for evaluation of the protective effects of transformed tobacco seeds against E. coli infection.

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Section: Resultsmentioning

confidence: 99%

Expression of verocytotoxicEscherichia coliantigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

et al. 2013

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“…Different studies showed the accumulation of heterologous antigenic proteins by using suitable promoters, specifically in tobacco seeds [3,4].…”

Section: Introductionmentioning

confidence: 99%

Tobacco Seeds By-Product as Protein Source for Piglets

Rossi¹,

Fusi²,

Baldi³

et al. 2013

OJVM

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Tobacco seed cake is a by-product with interesting characteristics for animal nutrition, due to its high protein content. The focus of this study is to evaluate if tobacco seed cake, administered in feed, can affect principal serum metabolic parameters of weaned piglets in order to establish if it can be used as both a delivery system of edible vaccines and an alternative protein source in piglets diet. A total of 48 weaned piglets were divided in two homogeneous groups for weight, control (CG) and treatment (TG); TG and CG were fed ad libitum with two isoenergetic and isoproteic experimental diets (CP: 17.68% CG and 17.64% TG) differentiated for the inclusion of 4% of tobacco seed cake in the TG diet in replacement of wheat bran and soybean meal. Growth performances were evaluated and feed intake was measured weekly. Blood samples were collected on days 0, 20 and 43 to evaluate hematocrit and principal metabolic parameters, in order to assess the health status of the animals. The administration of tobacco did not impaire health status and growth performance of piglets. The use of bioenergy co-products from non-food crops may represent a good approach creating an integration between biofuel and food production, with consequent benefits for food security and environmental impact.

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Plant‐Made Vaccines Targeting Enteric Pathogens—Safe Alternatives for Vaccination in Developing Countries

Trujillo,

Angulo

2024

Biotech & Bioengineering

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Enteric diseases by pathogenic organisms are one of the leading causes of death worldwide, particularly in low‐income countries. Despite antibiotics, access to clean water and vaccination are the most economically affordable options to prevent those infections and their health consequences. Vaccines, such as those approved for rotavirus and cholera, have played a key role in preventing several enteric diseases. However, vaccines for other pathogens are still in clinical trials. Distribution and cost remain significant barriers to vaccine access in developing regions due to poor healthcare infrastructure, cold‐chain requirements, and high production costs. Plant‐made vaccines offer a promising alternative to address these challenges. Plants can be easily grown, lowering production costs, and can be administered in oral forms, potentially eliminating cold‐chain dependency. Although there are some promising prototypes of vaccines produced in plants, challenges remain, including yields and achieving sufficient immunogenicity. This review aims to describe common enteric pathogens and available vaccines, followed by a strategic summary of plant‐made vaccine development and a discussion of plant‐made enteric vaccine prototypes. Trends to overcome the key challenges for plant‐made vaccines are identified and placed in perspective for the development of affordable and effective vaccines for populations at the highest risk of enteric diseases.

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Expression of flak flagellin from Salmonella Typhimurium in tobacco seeds

Cited by 3 publications

References 12 publications

Expression of verocytotoxicEscherichia coliantigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

Expression of verocytotoxicEscherichia coliantigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

Tobacco Seeds By-Product as Protein Source for Piglets

Plant‐Made Vaccines Targeting Enteric Pathogens—Safe Alternatives for Vaccination in Developing Countries

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