Characterization of SCAR markers of<i>Eimeria</i>spp. of domestic fowl and construction of a public relational database (The<i>Eimeria</i>SCARdb)

Fernandez, Sandra; Katsuyama, Angela M.; Kashiwabara, André Yoshiaki; Madeira, Alda Maria Backx Noronha; Durham, Alan Mitchell; Gruber, Arthur

doi:10.1111/j.1574-6968.2004.tb09754.x

Cited by 9 publications

(2 citation statements)

References 29 publications

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“…From the 1990s onward, researchers used fingerprinting methods based on PCR such as randomly amplified polymorphic DNAs (RAPDs) , amplified fragment length polymorphisms (AFLPs) , and sequence characterized amplified regions (SCARs) . These methods allowed different Eimeria species to be distinguished from one another by polymorphic molecular markers, and facilitated the construction of genetic linkage maps.…”

Section: Introductionmentioning

confidence: 99%

A simple, one‐tube assay for the simultaneous detection and diagnosis of ten Australian poultry Eimeria

Godwin

Morgan

2013

Electrophoresis

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Coccidiosis is a costly worldwide enteric disease of chickens caused by parasites of the genus Eimeria. At present, there are seven described species that occur globally and a further three undescribed, operational taxonomic units (OTUs X, Y, and Z) that are known to infect chickens from Australia. Species of Eimeria have both overlapping morphology and pathology and frequently occur as mixed-species infections. This makes definitive diagnosis with currently available tests difficult and, to date, there is no test for the detection of the three OTUs. This paper describes the development of a PCR-based assay that is capable of detecting all ten species of Eimeria, including OTUs X, Y, and Z in field samples. The assay is based on a single set of generic primers that amplifies a single diagnostic fragment from the mitochondrial genome of each species. This one-tube assay is simple, low-cost, and has the capacity to be high throughput. It will therefore be of great benefit to the poultry industry for Eimeria detection and control, and the confirmation of identity and purity of vaccine strains.

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Section: Introductionmentioning

confidence: 99%

A simple, one‐tube assay for the simultaneous detection and diagnosis of ten Australian poultry Eimeria

Godwin

Morgan

2013

Electrophoresis

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“…In the area of parasitology, the SCARs have been used to identify Eimeria spp. 14, Trypanosoma cruzi lineages 15, Taenia spp. 16, and isomorphic species in the Anopheles dirus complex 7, the vector for Plasmodium .…”

Section: Introductionmentioning

confidence: 99%

An effective sequence characterized amplified region‐PCR method derived from restriction site‐amplified polymorphism for the identification of femaleSchistosoma japonicumof zoonotic significance

Zhao

Lin

et al. 2010

Electrophoresis

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In the present study, restriction site-amplified polymorphism (RSAP) markers were used to examine the genetic variability of Schistosoma japonicum isolates from different endemic provinces in mainland China. Of the 45 pairs of primers screened, 10 RSAP markers showed a clear banding pattern with good resolution; however, only six exhibited a polymorphism among different isolates. Among six RSAP markers, one pair of primers (R8+R10) was able to differentiate male and female parasites, and amplified one constant specific band for female S. japonicum isolates. The specific band was recovered, re-amplified and sequenced, and a sequence of 162 bp was obtained. Based on this sequence, a pair of specific primers was designed and used to develop sequence characterized amplified region (SCAR)-PCR assay for identification and differentiation of female S. japonicum isolates. The SCAR-PCR assay allowed the specific identification of female S. japonicum, with no amplicons being amplified from male S. japonicum, Fasciola hepatica, Clonorchis sinensis, S. mansoni (male and female parasite). DNA sequencing confirmed the identity of the amplified products. The minimum amount of DNA detectable using SCAR-PCR assay was 0.3 ng for female S. japonicum. The SCAR-PCR was able to differentiate effectively the male and female S. japonicum worms collected from 12 geographical origins in eight endemic provinces, the gender of which was known based on the morphological and biological features. These results showed that SCAR-PCR provides an effective tool for the sex differentiation studies of S. japonicum, identification of female S. japonicum, diagnosis and epidemiological survey of S. japonicum infections in animals and human.

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Protozoal Infections

McDougald¹,

Cervantes²,

Jenkins³

et al. 2019

Diseases of Poultry

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Characterization of SCAR markers ofEimeriaspp. of domestic fowl and construction of a public relational database (TheEimeriaSCARdb)

Cited by 9 publications

References 29 publications

A simple, one‐tube assay for the simultaneous detection and diagnosis of ten Australian poultry Eimeria

A simple, one‐tube assay for the simultaneous detection and diagnosis of ten Australian poultry Eimeria

An effective sequence characterized amplified region‐PCR method derived from restriction site‐amplified polymorphism for the identification of femaleSchistosoma japonicumof zoonotic significance

Protozoal Infections

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