Characterization of SCCmec, spa types and Multi Drug Resistant of methicillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran

Abstract

Objectives Molecular typing such as spa typing is used to control and prevent Staphylococcus aureus widespread in hospitals and communities. Hence, the aim of this study was to find the most common types of S. aureus strain circulating in Shiraz via spa and SCCmec typing methods. Results Total of 159 S. aureus isolates were collected from two tertiary hospitals in Shiraz. Isolates were identified by biochemical tests. Antimicrobial susceptibility tests were performed by standard disk diffusion method and then genetic analysis of bacteria was performed using SCCmec and spa typing. In this study 31.4% of the isolates were methicillin-resistant S. aureus. The majority of isolates were SSCmec type III. Spa type t030 was the most prominent type among MRSA strains. For the first time in Iran, spa003, t386, t1877, t314, t186, t1816, t304, t325, t345 were reported in this study. It was shown that there is a possibility that these spa types are native to this region. Our findings showed that SCCmec II, III and IV disseminate from hospital to community and vice versa. Thus, effective monitoring of MRSA in hospital and community is necessary.

First report of methicillin-resistant Staphylococcus aureus carrying the mecC gene in human samples from Iran: prevalence and molecular characteristics

“…This evidence leads us to conclude that HA-MRSA strains collected in present survey may be linked to the ST239 clone. Similar to other studies [ 15 , [19] , [20] , [21] ], in the present study, cassette types I and II represented a minority, with frequencies of 7.5% and 10.1% respectively. Conversely, Chongtrakool et al.…”

The Differences in Antibiotic-resistance among Several Staphylococcus aureus strains in Iraq

“…Strain t304,and t386 showed sensitivity to ciprofloxacin, gentamycin and rifampin. This is similar to that reported in an Iranian study (34) .…”

Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019

“…SCC mec typing provides useful information regarding resistance to antimicrobials and the origin of S. aureus strains 57 . In our study, SCC mec IV and III were the most common SCC mec types, which is consistent with findings reported earlier 57 - 59 . In addition, the MRSA SCC mec type III strains exhibited higher multidrug resistance, whereas the MRSA SCC mec type IV were more multidrug-susceptible compared to other SCC mec types.…”