Characterization of Seashore Paspalum (Paspalum vaginatum Swartz) Germplasm by Transferred SSRs from Wheat, Maize and Sorghum

Abstract

One hundred and thirty SSR markers from wheat, maize and sorghum were screened for the transferability to Paspalum. The transfer rate was 67.5, 49.0 and 66.8% respectively. This would be a very efficient approach for DNA marker development for species which are not well studied molecularly. The polymorphism level for transferred SSR markers was 51.5% within species (Paspalum vaginatum) and 87.1% among Paspalum species. The high level of polymorphism is directly related to the high degree of heterozygosity maintained by its way of reproduction, i.e. self-incompatibility. Forty transferred polymorphic SSR markers were selected and used for characterization and evaluation of seventy-three Paspalum accessions. In total, 209 polymorphic bands were detected from these 40 SSR markers, with an average of five polymorphic bands per marker. The Paspalum accessions clustered into three major groups. Two very similar dendrograms can be generated from either 109 or 209 polymorphic bands. This led us to determine that 18 of the transferred SSR markers were sufficient for genetically differentiating the investigated germplasm accessions. The number of SSR markers required for germplasm characterization and evaluation is discussed. This is the first report of the transfer of SSR markers from major field crops to newly emerged environmental turfgrasses.

Development and Characterization of Seashore Paspalum SSR Markers

“…Fifty-four accessions are maintained at the USDA-ARS, Plant Genetic Resources Conservation Unit, Griffin, GA, approximately 300 accessions are maintained by P. Raymer at Griffin, GA, and an unknown number of accessions exist in a collection in Brazil maintained by Embrapa (Chen et al, 2005;Wang et al, 2006). Fifty-four accessions are maintained at the USDA-ARS, Plant Genetic Resources Conservation Unit, Griffin, GA, approximately 300 accessions are maintained by P. Raymer at Griffin, GA, and an unknown number of accessions exist in a collection in Brazil maintained by Embrapa (Chen et al, 2005;Wang et al, 2006).…”

Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae)

“…Both Japanese plum and apple cultivars have strong self-incompatibility (Okie and Weinberger, 1996;Hegedus, 2006) compared to the wax apple, which is a self-compatible species (Chantaranothai and Parnell, 1994). Self-incompatibility tends to maintain a high degree of heterogeneity in crops (Wang et al, 2006).…”

Utilização de microssatélites e RAPD na caracterização molecular de acessos de Paspalum urvillei Steudel

“…Neste estudo, foram utilizados primers desenhados para outras espécies, como milho (Zea mays), arroz (Oryza sativa) e sorgo (Sorghum bicolor), testados com sucesso em P. vaginatum por Wang et al (2006), primers utilizados em trevo-branco (Trifolium repens) por Kölliker et al (2001) e primers utilizados em azevém ( Lolium multiflorum L.) por Kubik et al (1999). Apesar do longo tempo de evolução das espécies, algumas semelhanças dos ancestrais comuns ainda se mantiveram, o que possibilitou a utilização de primers de seqüências repetitivas.…”

“…A reação de PCR para o SSR foi adaptada do protocolo descrito por Wang et al (2006), com modificações. O volume final de reação, de 15 µL, foi composto de 3 µL da solução de trabalho de DNA (15 ng/µL), 1,5 µL de tampão PCR 10X, 0,90 µL de MgCl 2 (50 mM), 0,6 µL de 10 mM de dNTP mix contendo 2,5 mM de cada um dos quatro nucleotídeos, 1,2 µL de primer (100 ng/µL), 0,27 µL de Taq DNA polimerase (5 U/µl) e água MiliQ esterilizada para completar o volume.…”

“…O volume final de reação, de 15 µL, foi composto de 3 µL da solução de trabalho de DNA (15 ng/µL), 1,5 µL de tampão PCR 10X, 0,90 µL de MgCl 2 (50 mM), 0,6 µL de 10 mM de dNTP mix contendo 2,5 mM de cada um dos quatro nucleotídeos, 1,2 µL de primer (100 ng/µL), 0,27 µL de Taq DNA polimerase (5 U/µl) e água MiliQ esterilizada para completar o volume. Utilizaram-se sete primers (Pv-1; Pv-3; Pv-53; M-15; TRSSRA01H11; TRSSRA02C03; TRSSRAXX31), de acordo com trabalhos anteriormente realizados com Lolium multiflorum L. (Kubik et al, 1999), Paspalum vaginatum Sw. (Wang et al, 2006) e Trifolium repens L. (Kölliker et al, 2001). …”