Characterization of selected fluorescamine-amino acid reaction products by high-performance liquid chromatography

McHugh, W.; Sandmann, Robert A.; Haney, W.G.; Sood, Sonia; Wittmer, D.P.

doi:10.1016/s0021-9673(00)89756-3

Cited by 27 publications

(1 citation statement)

References 13 publications

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“…For example, in the cases of Dansyl-Cl, Bansyl-Cl, and Mansyl-Cl (1-3), the reagents themselves and the reaction by-products fluoresce which we found to cause serious interference at the high sensitivity level needed for the direct analysis of, for example, seawater samples. Another reagent, fluorescamine (1-3), reacts rapidly and quantitatively with primary amines; however, the reaction products are rather bulky and, for amino acids, two fluorescent derivatives are formed (7).…”

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confidence: 99%

High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde

Lindroth¹,

Mopper²

1979

Anal. Chem.

1,816

757

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Using reversed-phase high performance liquid chromatography, 25 amino acids as o-phthaldialdehyde derivatives were determined with a 30-min sample turnover. The derivatization procedure is rapid, performed in aqueous medium, and has few transfer steps. The precision is ±0.5% at the 80-pmol level. Recoveries of standards added to urine samples were >98%. Intercallbration with an amino acid analyzer showed an agreement of >95 %. The detection limit Is about 50 fmol.The overall retention is controlled by the organic modifier, but second-order effects are ascribed to Ion repulsion between anions In the eluent and the ionized carboxylate on the derivatives. Clinical, biochemical, and environmental applications are given. Amino acids and ammonia were determined In 25

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confidence: 99%