Characterization of serum biomarkers during anaphylaxis in emergency department patients

Dass, Conor; Eyck, Patrick Ten; Ballas, Zuhair K.; Lee, Sangil

doi:10.1016/j.jaip.2020.05.010

Cited by 5 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL‐6 was also elevated in anaphylaxis reaction samples but did not reach significance level ( p = .068, Figure 1A). These findings are in agreement with other studies reporting an increase in IL‐6, IL‐10, TNFRI and CCL2 in patients with anaphylaxis to different elicitors 4–6 . However, when analysing the surgical control patients, we saw a tendency of an increased level of IL‐6, IL‐10 and TNFRI following the hypotensive response in these patients during surgery despite the non‐allergic mechanism (Table ).…”

Section: Patient Id Age At Reaction (Y)a Sex Ring and Messmer Reaction Grade Culprit Drug Time From Culprit Exposure To Reaction (Min) Resupporting

confidence: 92%

“…We did not detect a significant increase in IL‐6 during anaphylaxis as reported by others who have compared reaction samples with baseline samples of a control group instead of with the patient's own baseline level 4,6 . This highlights the importance of including the patient's own baseline sample as the reference sample as is already standard practice for tryptase.…”

Section: Patient Id Age At Reaction (Y)a Sex Ring and Messmer Reaction Grade Culprit Drug Time From Culprit Exposure To Reaction (Min) Resupporting

confidence: 54%

See 1 more Smart Citation

Comparing baseline and reaction samples of perioperative anaphylaxis patients reveals IL‐6 and CCL2 as potential biomarkers

Callesen

Poulsen

Garvey

et al. 2021

Clin Experimental Allergy

View full text Add to dashboard Cite

Section: Patient Id Age At Reaction (Y)a Sex Ring and Messmer Reaction Grade Culprit Drug Time From Culprit Exposure To Reaction (Min) Resupporting

confidence: 92%

Section: Patient Id Age At Reaction (Y)a Sex Ring and Messmer Reaction Grade Culprit Drug Time From Culprit Exposure To Reaction (Min) Resupporting

confidence: 54%

Comparing baseline and reaction samples of perioperative anaphylaxis patients reveals IL‐6 and CCL2 as potential biomarkers

Callesen

Poulsen

Garvey

et al. 2021

Clin Experimental Allergy

View full text Add to dashboard Cite

“…It is necessary to further compare the utility of such biomarkers to tryptase, the common biomarker for acute anaphylaxis. No other significant differences were detected for other cytokines, such as IL-1B, IL-2, IL-4, IL-8, IL-12, IL-13, IL-18, TNFα, and for complement C5a or adhesion molecule ICAM-1 [ 97 ]. Activation of the coagulation/fibrinolytic system usually appears together with the activation of the complement system, which is considered a triggering mechanism in severe anaphylaxis.…”

Section: Biomarkersmentioning

confidence: 99%

State-of-the-Art on Biomarkers for Anaphylaxis in Obstetrics

Simionescu

Stănescu

Popescu

2021

Life

View full text Add to dashboard Cite

Anaphylaxis is an unpredictable systemic hypersensitivity reaction and constitutes a high risk of maternal and fetal morbidity and mortality when occurring during pregnancy. Currently, the acute management of anaphylaxis is based on clinical parameters. A total serum tryptase is only used to support an accurate diagnosis. There is a need to detect other biomarkers to further assess high-risk patients in obstetrics. Our objective is to present biomarkers in this complex interdisciplinary approach beyond obstetrician and anaesthetic management. Candidate biomarkers derive either from mediators involved in immunopathogenesis or upcoming molecules from systems biology and proteomics. Serum tryptase is determined by singleplex immunoassay method and is important in the evaluation of anaphylactic mast cell degranulation but also in the assessment of other risk factors for anaphylaxis such as systemic mastocytosis. Another category of biomarkers investigates the IgE-mediated sensitization to triggers potentially involved in the etiology of anaphylaxis in pregnant women, using singleplex or multiplex immunoassays. These in vitro tests with natural extracts from foods, venoms, latex or drugs, as well as with molecular allergen components, are useful because in vivo allergy tests cannot be performed on pregnant women in such a major medical emergency due to their additional potential risk of anaphylaxis.

show abstract

“…In addition, the relatively late diagnostic window for serum tryptase between 30 -120 minutes after the onset of first anaphylaxis symptoms is too late concerning the necessity of rapid treatment initiation. Therefore multiple efforts have been undertaken in identifying other potential biomarkers of anaphylaxis (5)(6)(7)(8) miRNAs are small non-coding RNAs that regulate gene expression usually by silencing transcription (9). Their use as serological biomarkers has been proposed in various diseases (10).…”

Section: Introductionmentioning

confidence: 99%

Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis

Francuzik¹,

Pažur²,

Dalke³

et al. 2022

JCI Insight

View full text Add to dashboard Cite

Background: There is a need to support the diagnosis of anaphylaxis by objective markers. miRNAs are promising non-coding RNA species that may serve as serological biomarkers, but their use in diagnosing anaphylaxis has not been systematically studied. We aimed to comprehensively investigate serum biomarker profiles (proteins, lipids, and miRNAs) to support the diagnosis of anaphylaxis.Methods: Adult patients admitted to the emergency room with a diagnosis of anaphylaxis (< 3 hours) were included. Blood samples were taken upon emergency room arrival and one month later.Results: Next-generation sequencing of 18 samples (6 anaphylaxis patients in acute and non-acute condition, respectively, and 6 healthy controls) identified hsa-miR-451a to be elevated during anaphylaxis and verified by RT-qPCR in the remaining cohort. The random forest classifier enabled us to classify anaphylaxis with high accuracy using a composite model. We identified tryptase, 9α,11β-PGF2, apolipoprotein A1, and hsa-miR-451a as serological biomarkers of anaphylaxis. These predictors qualified as serological biomarkers individually but performed better in combination. Conclusion:Unexpectedly, hsa-miR-451a was identified as the most relevant biomarker in our data set. We were also able to distinguish between patients with a history of anaphylaxis and healthy individuals with higher accuracy than any other available model. Future studies will need to verify miRNA biomarker utility in real-life clinical settings.

show abstract

Characterization of serum biomarkers during anaphylaxis in emergency department patients

Cited by 5 publications

References 7 publications

Comparing baseline and reaction samples of perioperative anaphylaxis patients reveals IL‐6 and CCL2 as potential biomarkers

Comparing baseline and reaction samples of perioperative anaphylaxis patients reveals IL‐6 and CCL2 as potential biomarkers

State-of-the-Art on Biomarkers for Anaphylaxis in Obstetrics

Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis

Contact Info

Product

Resources

About