Characterization of Somatic Hybrids Solanum Bulbocastanum + Dihaploid Solanum Tuberosum

Greplová, Marie; Polzerová, H.; Patzak, Josef; Mazáková, J.; Bouma, J.W.; Domkářová, J.

doi:10.17660/actahortic.2012.961.74

“…Somatic hybridization mediated by protoplast fusion can be a useful breeding tool for introducing valuable traits from related species (Arimura et al, 2018;Temple et al, 1992;Wachocki et al, 1991). Favorable traits such as disease resistance (Smyda et al, 2013;Thieme et al, 2008) and drought tolerance (Greplova et al, 2012) were transmitted to the cultivated potato from wild species. Similar approaches have been applied to develop potato somatic hybrids with enhanced disease resistance using wild potato species (Cardi et al, 1999;Chen et al, 2013;Iovene et al, 2007;Kemble et al, 1986;Nouri-Ellouz et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Genome Recombination in Somatic Hybrids of Solanum commersonii and S. tuberosum

Cho,

Lee,

Lee

et al. 2024

Preprint

0

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Interspecific somatic hybridization has been performed in potato breeding experiments to increase plant resistance against biotic and abiotic stress conditions. We analyzed the mitochondrial and plastid genomes and 45S nuclear ribosomal DNA (45S rDNA) for the cultivated potato (S. tuberosum, St), wild potato (S. commersonii, Sc), and their somatic hybrid (StSc). Complex genome components and structure, such as the hybrid form of 45S rDNA in StSc, unique plastome in Sc, and recombinant mitogenome were identified. However, the mitogenome exhibited dynamic multipartite structures in both species as well as in the somatic hybrid. In St, the mitogenome is 756,058 bp and is composed of five subgenomes ranging from 297,014 to 49,171 bp in St. In Sc, it is 552,103 bp long and is composed of two sub-genomes of 338,427 and 213,676 bp length. StSc has 447,645 bp long mitogenome with two subgenomes of length 398,439 and 49,206 bp. The mitogenome structure exhibited dynamic recombination mediated by tandem repeats; however, it contained highly conserved genes in the three species. Among the 35 protein-coding genes of the StSc mitogenome, 21 were identical for all the three species, and 12 and 2 were unique in Sc and St, respectively. The recombinant mitogenome might be derived from homologous recombination between both species during somatic hybrid development.

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“…Favorable traits such as disease resistance 20,21 and drought tolerance 22 were transmitted to the cultivated potato from wild species. Similar approaches have been applied to develop potato somatic hybrids with enhanced disease resistance using wild potato species [23][24][25][26][27] .…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Genome Recombination in Somatic Hybrids of Solanum Commersonii and S. Tuberosum

Cho

¹

,

Lee

²

,

Lee

³

et al. 2021

Preprint

View full text Add to dashboard Cite

Interspecific somatic hybridization has been performed in potato breeding experiments to increase plant resistance against biotic and abiotic stress conditions. We analyzed the mitochondrial and plastid genomes and 45S nuclear ribosomal DNA (45S rDNA) for the cultivated potato (S. tuberosum, St), wild potato (S. commersonii, Sc), and their somatic hybrid (StSc). Complex genome components and structure, such as the hybrid form of 45S rDNA in StSc, unique plastome in Sc, and recombinant mitogenome were identified. However, the mitogenome exhibited dynamic multipartite structures in both species as well as in the somatic hybrid. In St, the mitogenome is 756,058 bp and is composed of five subgenomes ranging from 297,014 to 49,171 bp in St. In Sc, it is 552,103 bp long and is composed of two sub-genomes of 338,427 and 213,676 bp length. StSc has 447,645 bp long mitogenome with two subgenomes of length 398,439 and 49,206 bp. The mitogenome structure exhibited dynamic recombination mediated by tandem repeats; however, it contained highly conserved genes in the three species. Among the 35 protein-coding genes of the StSc mitogenome, 21 were identical for all the three species, and 12 and 2 were unique in Sc and St, respectively. The recombinant mitogenome might be derived from homologous recombination between both species during somatic hybrid development.

show abstract

Mitochondrial genome recombination in somatic hybrids of Solanum commersonii and S. tuberosum

Cho

¹

,

Lee

²

,

Lee

³

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Interspecific somatic hybridization has been performed in potato breeding experiments to increase plant resistance against biotic and abiotic stress conditions. We analyzed the mitochondrial and plastid genomes and 45S nuclear ribosomal DNA (45S rDNA) for the cultivated potato (S. tuberosum, St), wild potato (S. commersonii, Sc), and their somatic hybrid (StSc). Complex genome components and structure, such as the hybrid form of 45S rDNA in StSc, unique plastome in Sc, and recombinant mitogenome were identified. However, the mitogenome exhibited dynamic multipartite structures in both species as well as in the somatic hybrid. In St, the mitogenome is 756,058 bp and is composed of five subgenomes ranging from 297,014 to 49,171 bp. In Sc, it is 552,103 bp long and is composed of two sub-genomes of 338,427 and 213,676 bp length. StSc has 447,645 bp long mitogenome with two subgenomes of length 398,439 and 49,206 bp. The mitogenome structure exhibited dynamic recombination mediated by tandem repeats; however, it contained highly conserved genes in the three species. Among the 35 protein-coding genes of the StSc mitogenome, 21 were identical for all the three species, and 12 and 2 were unique in Sc and St, respectively. The recombinant mitogenome might be derived from homologous recombination between both species during somatic hybrid development.

show abstract

Characterization of Somatic Hybrids Solanum Bulbocastanum + Dihaploid Solanum Tuberosum

Cited by 3 publications

References 0 publications

Mitochondrial Genome Recombination in Somatic Hybrids of Solanum commersonii and S. tuberosum

Mitochondrial Genome Recombination in Somatic Hybrids of Solanum commersonii and S. tuberosum

Mitochondrial Genome Recombination in Somatic Hybrids of Solanum Commersonii and S. Tuberosum

Mitochondrial genome recombination in somatic hybrids of Solanum commersonii and S. tuberosum

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