Characterization of spiral ganglion neurons cultured on silicon micro-pillar substrates for new auditory neuro-electronic interfaces

Mattotti, Marta; Micholt, Liesbeth; Braeken, Dries; Kovačić, Damir

doi:10.1088/1741-2560/12/2/026001

Cited by 26 publications

(55 citation statements)

References 49 publications

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“…However, as the spacing between pillar structures increased above 3.2 μm, the effect of topographical guidance diminished as neurites started to partially grow between the pillar structures. Similar results were obtained in other studies with different types of neurons14161719. Only a few studies have examined the impact of the substrate architecture on the growth of DRG neurons and directionality of its neurites.…”

Section: Discussionsupporting

confidence: 87%

“…On isotropic structures, neurites follow existing contacts (pili, pits), but if a new contact is nearby they extend to this contact resulting in a highly aligned distribution of extending neurites1417353637. In our case, neurite alignment along 30°, 90° and 150° radial angles was only obvious in the first two bins.…”

Section: Discussionmentioning

confidence: 51%

“…For this purpose, numerous isotropic and anisotropic, micro- and nano-patterned substrates of different rigidity13 and geometrical features such as shape (e.g. vertical pili, grooves-lines), size and spatial density1415161718 were produced and used in studies of cellular behavior. The knowledge gained by observing the interaction of neurons with appropriate substrates may allow us to predict or even control their behavior through the fabrication of substrates with precisely defined physical characteristics.…”

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confidence: 99%

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Characterization of dorsal root ganglion neurons cultured on silicon micro-pillar substrates

Repić

Madirazza

Bektur

et al. 2016

Sci Rep

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Our study focuses on characterization of dorsal root ganglion (DRG) neurons cultured on silicon micro-pillar substrates (MPS) with the ultimate goal of designing micro-electrode arrays (MEAs) for successful electrophysiological recordings of DRG neurons. Adult and neonatal DRG neurons were cultured on MPS and glass coverslips for 7 days in vitro. DRG neuronal distribution and morphometric analysis, including neurite alignment and length, was performed on MPS areas with different pillar width and spacing. We showed that MPS provide an environment for growth of adult and neonatal DRG neurons as permissive as control glass surfaces. Neonatal DRG neurons were present on MPS areas with narrow pillar spacing, while adult neurons preferred wider pillar spacing. Compared to the control glass surfaces the neonatal and adult DRG neurons in regions with narrow pillar spacing range developed a smaller number of longer neurites. In the same area, neurites were preferentially oriented along three directional axes at 30°, 90° and 150°. MPS architecture influenced growth directionality of all main DRG neuronal subtypes. We can conclude that specific micro-pillar substrate topography affects the morphology of DRG neurons. This knowledge can enable development of MEAs with precisely defined physical features for various neuroscience applications.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 51%

mentioning

confidence: 99%

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Characterization of dorsal root ganglion neurons cultured on silicon micro-pillar substrates

Repić

Madirazza

Bektur

et al. 2016

Sci Rep

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Section: Introductionmentioning

confidence: 99%

“…1,2 Among several different topographies, micrometer sized pillars have been used for different purposes such as to promote neuronal axonal growth and orientation [3][4][5] and cellular differentiation. [5][6][7][8] Smaller diameter pillars, nanopillars, are applied for different purposes, for example, examination of neuronal development 9 and pinning neurons. [10][11][12] Nanopillars have also been used to access cellular membranes for electroporation, 13,14 which led them to the successful measurement of neuronal intracellular action potentials.…”

Section: Introductionmentioning

confidence: 99%

Neuronal Growth on a-Si and Au Nanopillars

Kasai

Filip

et al. 2016

Electrochemistry

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Neuronal patterning is useful for understanding signal propagation between neurons as well as for biosensors and cell-based assays. The patterning of living cells has been made possible by employing surface physicochemical and topographic features. This study investigated neuronal growth on patterned nanopillars. Rat cortical neurons were cultivated on quartz substrates with amorphous silicon (a-Si) and Au pillars 100 and 500 nm in diameter. The neurites grew better with the larger diameter pillars, and the partly-selective neurite growth was observed for a-Si pillars but not for Au pillars. These results reveal the possibility of controlling neuronal growth by using a-Si nanopillars.

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A Bioresorbable and Conductive Scaffold Integrating Silicon Membranes for Peripheral Nerve Regeneration

Sun,

Guan,

Yang

et al. 2023

Adv Healthcare Materials

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Peripheral nerve injury represents one of the most common types of traumatic damage, severely impairing motor and sensory functions, and posttraumatic nerve regeneration remains a major challenge. Electrical cues are critical bioactive factors that promote nerve regrowth, and bioartificial scaffolds incorporating conductive materials to enhance the endogenous electrical field have been demonstrated to be effective. The utilization of fully biodegradable scaffolds can eliminate material residues, avoid the need for a second surgery and circumvent the need for secondary retrieval procedures. Here, we propose a fully bioresorbable and conductive nerve scaffold integrating N‐type silicon (Si) membranes, which can deliver both structural guidance and electrical cues for the repair of nerve defects. The entire scaffold is fully biodegradable, and the introduction of N‐type Si can significantly promote the proliferation and production of neurotrophic factors in Schwann cells and enhance the calcium activity of dorsal root ganglion (DRG) neurons. The conductive scaffolds enable accelerated nerve regeneration and motor functional recovery in rodents with sciatic nerve transection injuries. This work sheds light on the advancement of bioresorbable and electrically active materials to achieve desirable neural interfaces and improved therapeutic outcomes, offering essential strategies for regenerative medicine.This article is protected by copyright. All rights reserved

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Characterization of spiral ganglion neurons cultured on silicon micro-pillar substrates for new auditory neuro-electronic interfaces

Abstract: These results indicate that silicon micro-pillar substrates create a permissive environment for the growth of primary auditory neurons promoting neurite sprouting and are a promising technology for future high-density three-dimensional CMOS-based auditory neuro-electronic interfaces.

Cited by 26 publications

References 49 publications

Characterization of dorsal root ganglion neurons cultured on silicon micro-pillar substrates

Characterization of dorsal root ganglion neurons cultured on silicon micro-pillar substrates

Neuronal Growth on a-Si and Au Nanopillars

A Bioresorbable and Conductive Scaffold Integrating Silicon Membranes for Peripheral Nerve Regeneration

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