Characterization of Staphylococcal Cassette Chromosome
            <i>mec</i>
            Elements from Methicillin-Resistant
            <i>Staphylococcus pseudintermedius</i>
            Infections in Australian Animals

Worthing, Kate A.; Schwendener, Sybille; Perreten, Vincent; Saputra, Sugiyono; Coombs, Geoffrey W.; Pang, Stanley; Davies, Mark R.; Abraham, Sam; Trott, Darren J.; Norris, Jacqueline M.

doi:10.1128/msphere.00491-18

Cited by 21 publications

(35 citation statements)

References 33 publications

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“…4 and Supplementary Data 1). These results were similar to those identified in veterinary MRSP in Australia 27 . Their locations in the phylogenetic tree show that the MRSP phenotype has been derived from at least 13 independent acquisitions of different SCCmec elements.…”

Section: Resultssupporting

confidence: 90%

Population genomics of Staphylococcus pseudintermedius in companion animals in the United States

et al. 2020

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Staphylococcus pseudintermedius is a commensal bacterium and a major opportunistic pathogen of dogs. The emergence of methicillin-resistant S. pseudintermedius (MRSP) is also becoming a serious concern. We carried out a population genomics study of 130 clinical S. pseudintermedius isolates from dogs and cats in the New England region of the United States. Results revealed the co-circulation of phylogenetically diverse lineages that have access to a large pool of accessory genes. Many MRSP and multidrug-resistant clones have emerged through multiple independent, horizontal acquisition of resistance determinants and frequent genetic exchange that disseminate DNA to the broader population. When compared to a Texas population, we found evidence of clonal expansion of MRSP lineages that have disseminated over large distances. These findings provide unprecedented insight into the diversification of a common cutaneous colonizer of man's oldest companion animal and the widespread circulation of multiple high-risk resistant clones.

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Section: Resultssupporting

confidence: 90%

Population genomics of Staphylococcus pseudintermedius in companion animals in the United States

et al. 2020

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“…8,14 These results confirm a link between the SCCmec and CC in MRSP, as previously described. 8 We found MRSP isolates susceptible to amoxicillin/clavulanic acid and cefalotin across all CCs and SCCmec types. CC71-SCCmecII-III isolates showed higher levels of resistance to oxacillin, ampicillin, amoxicillin/clavulanic acid and cefalotin, whereas SCCmecIV was associated with low-level resistance or even susceptibility to several b-lactams.…”

Section: Discussionsupporting

confidence: 90%

“…[4][5][6] In S. pseudintermedius, similar associations have been suggested. 7,8 Screening for methicillin resistance in S. pseudintermedius is performed by oxacillin susceptibility testing using a considerably lower resistance breakpoint (R 0.5 mg/L) than for S. aureus (R 4 mg/L). 9 Oxacillin-resistant isolates should be reported as resistant to all b-lactams according to an expert rule established for S. aureus to minimize major errors of antimicrobial susceptibility testing (i.e.…”

Section: Introductionmentioning

confidence: 99%

Specific staphylococcal cassette chromosome mec (SCCmec) types and clonal complexes are associated with low-level amoxicillin/clavulanic acid and cefalotin resistance in methicillin-resistant Staphylococcus pseudintermedius

Wegener

Damborg

Guardabassi

et al. 2019

Journal of Antimicrobial Chemotherapy

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Background Staphylococcus pseudintermedius is a common pathogen in dogs and methicillin resistance has emerged over recent decades. According to the current guidelines, S. pseudintermedius displaying oxacillin resistance should be reported as resistant to all β-lactams. Objectives To identify possible associations between β-lactam resistance levels and clonal complexes (CCs) and/or staphylococcal cassette chromosome mec (SCCmec) types in methicillin-resistant S. pseudintermedius (MRSP). Methods MICs of oxacillin, penicillin, ampicillin, amoxicillin/clavulanic acid and cefalotin were determined by broth microdilution for 86 clinical canine MRSP isolates from Denmark and the Netherlands. PCR and sequencing were used for SCCmec typing and MLST. Results Isolates belonged to CC71 (n = 36), CC258 (n = 33), CC45 (n = 11), CC68 (n = 1) and five singleton STs. SCCmecII-III was exclusively found in CC71 and SCCmecIV was significantly associated with CC258. SCCmecV and non-typeable SCCmec types occurred in 4 and 14 isolates, respectively. SCCmecIV was associated with lower MICs of oxacillin (<2 mg/L), ampicillin (<8 mg/L) and amoxicillin/clavulanic acid (<4 mg/L) and with susceptibility to cefalotin (<4 mg/L). All isolates harbouring SCCmecV were susceptible to cefalotin as well. Conclusions SCCmec types were associated with different CCs and with either high- or low-level resistance to different β-lactams. The finding of amoxicillin/clavulanic acid (20%) and cefalotin (70%) in vitro susceptibility across all CCs might have clinical implications, since amoxicillin/clavulanic acid and first-generation cephalosporins are first-choice antibiotics for treatment of S. pseudintermedius infections. Pharmacokinetic/pharmacodynamic and clinical outcome studies are warranted to evaluate the in vivo efficacy of these β-lactams for treatment of MRSP infections.

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“…Some S. aureus and coagulase-negative Staphylococcus isolates carry a mecA homolog, mecC , which has been recently reported carried by SCC mec XI [6, 7]. The classification of SCC mec elements is complex, given that there are composite cassettes and pseudo-SCC mec elements that do not harbor ccr genes [8]. While in S. aureus the structure of SCC mec elements has been shown to be relatively stable, in MRSP the SCC mec elements showed high genetic diversity [4].…”

Section: Introductionmentioning

confidence: 99%

“…Several dominant MRSP lineages have been identified in the world, including ST45, ST68 and ST71 [8], but the molecular epidemiology of MRSP clones circulating in Argentina has not been examined.…”

Section: Introductionmentioning

confidence: 99%

Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina

et al. 2019

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Background Staphylococcus pseudintermedius is the leading cause of pyoderma in dogs and the frequent use of antimicrobial treatment is associated to the development of resistance to nearly all classes of antibiotics. Despite S. pseudintermedius significance, our understanding of the molecular mechanism of β-lactam resistance and its genetic diversity remains limited. We aimed to: i ) determine the phenotypic resistance profile of methicillin resistant Staphylococcus pseudintermedius (MRSP) isolated from infected dogs in three different veterinary hospitals in Buenos Aires, Argentina; ii ) identify the SCC mec elements and resistance genes; and iii ) analyze the clonal relationship between isolates and in regard of dominant lineages found in the world. Results In addition to the differential levels of β-lactam resistance, MRSP isolates ( n = 10) showed resistance to 5–6 families of antibiotics, and were therefore categorized as multidrug-resistant. All the isolates were variant of SCC mec V homologous to S. aureus ; additional SCC mec Finder analysis classified five of the genomes as SCC mec type V (5C2&5) with mecA (encodes for PBP2a) , mecRI and mecI and all the genes closely related to the reference SCC mec type V S. aureus TSGH17 strain. In the remaining five strains, mecA was present, although other genes associated with SCC mec V including mecR1 and mecI were missing. PBP2a was inducible in low level resistance strains (MRSP 8151), and constitutively expressed in MRSP 8150, suggesting different mecA regulatory mechanisms. MRSP isolates showed significant genetic diversity: eight PFGE clonal types and six multilocus-sequence typing (MLST) sequence types (STs) (339, 649, 919, 920, 921 and 922), including four new STs genetically distinct from STs reported in other geographic areas. Comparative genomics and phylogenetic analyses of the MRSP showed a correlation between the genetic content and the phenotypes, and established the genetic relationship between the isolates. Conclusions MRSP could be a threat to animal health due to it concerning level of antimicrobial resistance. Our study highlights genetic and epidemiological aspects of multidrug-resistant MRSP strains from Argentina showing high degree of correlation between the resistance genes and the phenotype of the isolates and, furthermore, they appeared evolutionary closer to major worldwide reported ST68 and...

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Characterization of Staphylococcal Cassette Chromosome mec Elements from Methicillin-Resistant Staphylococcus pseudintermedius Infections in Australian Animals

Cited by 21 publications

References 33 publications

Population genomics of Staphylococcus pseudintermedius in companion animals in the United States

Population genomics of Staphylococcus pseudintermedius in companion animals in the United States

Specific staphylococcal cassette chromosome mec (SCCmec) types and clonal complexes are associated with low-level amoxicillin/clavulanic acid and cefalotin resistance in methicillin-resistant Staphylococcus pseudintermedius

Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina

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