Characterization of STIP, a multi-domain nuclear protein, highly conserved in metazoans, and essential for embryogenesis in Caenorhabditis elegans

Ji, Qiongmei; Huang, Cheng‐Han; Peng, Jianbin; Hashmi, Sarwar; Ye, Tianzhang; Chen, Ying

doi:10.1016/j.yexcr.2007.01.003

Cited by 10 publications

(37 citation statements)

References 32 publications

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“…TFIP11 has been identified as a constituent of the spliceosome in several proteomic studies (Jurica et al, 2002;Makarov et al, 2002;Rappsilber et al, 2002;Zhou et al, 2002) and is a member of the SEPTIN AND TUFTELIN INTERACTING PROTEIN (STIP) family of genes. STIP genes are present in one copy in animal genomes and, at least in Caenorhabditis elegans, STIP is essential for viability (Ji et al, 2007). Here, we report the role of an Arabidopsis thaliana TFIP11 homolog in efficient splicing of genes that act within the plant circadian clock.…”

Section: Introductionmentioning

confidence: 90%

“…Sequencing of candidate loci led to the identification of a G-to-A transition within the gene At1g17070 that is predicted to generate a premature termination codon (PTC; Figure 1B). At1g17070 shares significant homology with STIP genes identified in other metazoa (Ji et al, 2007), and we therefore designated our mutant stipl1-1. As a lesion in STIPL1 appeared to be responsible for the observed long-period circadian phenotype, we identified an additional T-DNA insertion line within the STIPL1 locus from the SAIL collection (Sessions et al, 2002) and renamed this allele stipl1-2 ( Figure 1B).…”

Section: Stipl1 Is a Member Of A Conserved Protein Familymentioning

confidence: 99%

“…Sequence analysis of STIPL1 reveals that it shares significant homology with the STIP family of proteins (see Supplemental Data Set 1 online; Aravind and Koonin, 1999;Svec et al, 2004;Wang and Brendel, 2004;Ji et al, 2007) The human STIP homolog, TFIP11, has been identified as a component of spliceosomal complexes in many proteomic studies (Jurica et al, 2002;Makarov et al, 2002;Rappsilber et al, 2002;Zhou et al, 2002), thereby implicating these proteins in pre-mRNA splicing. STIPL1 is highly similar to other STIP family members, containing three conserved domains ( Figure 1B) and sharing 32% overall amino acid identity and 49% similarity when compared with the human protein.…”

Section: Stipl1 Is a Member Of A Conserved Protein Familymentioning

confidence: 99%

“…Here, we report the role of an Arabidopsis thaliana TFIP11 homolog in efficient splicing of genes that act within the plant circadian clock. Arabidopsis SPLICEOSOMAL TIMEKEEPER LOCUS1 (STIPL1) and its paralog, STIPL2, share homology with members of the STIP family (Ji et al, 2007), but only mutation of STIPL1 induces a circadian defect under free-running conditions. Such data suggest a specialization of function following duplication of the ancestral gene.…”

Section: Introductionmentioning

confidence: 99%

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Mutation of Arabidopsis SPLICEOSOMAL TIMEKEEPER LOCUS1 Causes Circadian Clock Defects

et al. 2012

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The circadian clock plays a crucial role in coordinating plant metabolic and physiological functions with predictable environmental variables, such as dusk and dawn, while also modulating responses to biotic and abiotic challenges. Much of the initial characterization of the circadian system has focused on transcriptional initiation, but it is now apparent that considerable regulation is exerted after this key regulatory step. Transcript processing, protein stability, and cofactor availability have all been reported to influence circadian rhythms in a variety of species. We used a genetic screen to identify a mutation within a putative RNA binding protein (SPLICEOSOMAL TIMEKEEPER LOCUS1 [STIPL1]) that induces a long circadian period phenotype under constant conditions. STIPL1 is a homolog of the spliceosomal proteins TFP11 (Homo sapiens) and Ntr1p (Saccharomyces cerevisiae) involved in spliceosome disassembly. Analysis of general and alternative splicing using a high-resolution RT-PCR system revealed that mutation of this protein causes less efficient splicing of most but not all of the introns analyzed. In particular, the altered accumulation of circadian-associated transcripts may contribute to the observed mutant phenotype. Interestingly, mutation of a close homolog of STIPL1, STIP-LIKE2, does not cause a circadian phenotype, which suggests divergence in function between these family members. Our work highlights the importance of posttranscriptional control within the clock mechanism.

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Section: Introductionmentioning

confidence: 90%

Section: Stipl1 Is a Member Of A Conserved Protein Familymentioning

confidence: 99%

Section: Stipl1 Is a Member Of A Conserved Protein Familymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mutation of Arabidopsis SPLICEOSOMAL TIMEKEEPER LOCUS1 Causes Circadian Clock Defects

et al. 2012

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“…Sip1/tuftelin‐interacting proteins can self‐aggregate into large rod‐shaped polymers termed stiposomes in the nucleoplasm 7. In Caenorhabditis elegans , the knockdown of STIP via RNA interference causes developmental arrest of early embryos at the 16‐cell stage followed by 100% lethality.…”

Section: Introductionmentioning

confidence: 99%

STIP overexpression confers oncogenic potential to human non‐small cell lung cancer cells by regulating cell cycle and apoptosis

Tang

Yan

Song

et al. 2015

J Cellular Molecular Medi

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Sip1/tuftelin‐interacting protein (STIP), a multidomain nuclear protein, is a novel factor associated with the spliceosome, yet its role and molecular function in cancer remain unknown. In this study, we show, for the first time, that STIP is overexpressed in non‐small cell lung cancer (NSCLC) tissues compared to adjacent normal lung tissues. The depletion of endogenous STIP inhibited NSCLC cell proliferation in vitro and in vivo, caused cell cycle arrest and induced apoptosis. Cell cycle arrest at the G2/M phase was associated with the expression and activity of the cyclin B1‐CDK1 (cyclin‐dependent kinase 1) complex. We also provide evidence that STIP knockdown induced apoptosis by activating both caspase‐9 and caspase‐3 and by altering the Bcl‐2/Bax expression ratio. RNA sequencing data indicated that the MAPK mitogen‐activated protein kinases, Wnt, PI3K/AKT, and NF‐κB (nuclear factor kappa‐light‐chain‐enhancer of activated B cells) signalling pathways might be involved in STIP‐mediated tumour regulation. Collectively, these results suggest that STIP may be a novel potential diagnostic and therapeutic target for NSCLC.

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Differential protein expression in Spirometra erinacei according to its development in its final host

Kim

Sohn

et al. 2009

Parasitol Res

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This study was undertaken to identify genes involved in the growth and development of Spirometra erinacei larvae, an intestinal tapeworm of cats and dogs, within the final host. The differential protein expression at three different stages of S. erinacei, the plerocercoid larvae, 8-day-old juveniles, and adults, was compared using two-dimensional electrophoresis. Specifically or highly expressed proteins in juvenile worms were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MS)/MS. The proteome map of larvae showed fewer protein spots than juveniles or adults, whereas juveniles or adults revealed a similar protein expression profile. Eight juvenile-specific and five upregulated proteins of juveniles were identified and matched to proteins of known biological functions. These were grouped into several categories of functionally related proteins: DNA/RNA metabolism, cell trafficking, cytoskeleton, protein processing and degradation, energy metabolism, and oxidative stress. Our results give an overview of the growth and development mechanisms of cestodes within the final host and extend our understanding of parasite biology in the host-parasite relationship.

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Characterization of STIP, a multi-domain nuclear protein, highly conserved in metazoans, and essential for embryogenesis in Caenorhabditis elegans

Cited by 10 publications

References 32 publications

Mutation of Arabidopsis SPLICEOSOMAL TIMEKEEPER LOCUS1 Causes Circadian Clock Defects

Mutation of Arabidopsis SPLICEOSOMAL TIMEKEEPER LOCUS1 Causes Circadian Clock Defects

STIP overexpression confers oncogenic potential to human non‐small cell lung cancer cells by regulating cell cycle and apoptosis

Differential protein expression in Spirometra erinacei according to its development in its final host

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