Characterization of subpopulations and immune-related parameters of hemocytes in the green-lipped mussel Perna viridis

Wang, Youji; Hu, Menghong; Chiang, Michael; Shin, Paul K.S.; Cheung, S.G.

doi:10.1016/j.fsi.2011.08.024

Cited by 67 publications

(37 citation statements)

References 56 publications

(123 reference statements)

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“…Meanwhile, the basophilic granulocytes of M. edulis and M. galloprovincialis were shown to have lower phagocytic activity and fewer lysosomal enzymes than the eosinophilic granular hemocytes [14,18,23,38]. The lysosomal content of the granulocytes (corresponding to the EGs in the present study) was reported to be greater than that of the hyalinocytes (the BGs in the present study) in Perna viridis [41], Mya arenaria [42], Haliotis discus discus, and Turbo cornutus [43]. Moreover, the differences in the gravitational densities between the BGs and EGs (Table 2) may reflect differences between these two cell types in the amounts of granules (i.e., lysosomes) they possess.…”

Section: Discussionsupporting

confidence: 39%

Phagocytic activities of hemocytes from the deep-sea symbiotic mussels Bathymodiolus japonicus, B. platifrons, and B. septemdierum

Tame

Yoshida

Ohishi

et al. 2015

Fish & Shellfish Immunology

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Deep-sea mytilid mussels harbor symbiotic bacteria in their gill epithelial cells that are horizontally or environmentally transmitted to the next generation of hosts. To understand the immune defense system in deep-sea symbiotic mussels, we examined the hemocyte populations of the symbiotic Bathymodiolus mussel species Bathymodiolus japonicus, Bathymodiolus platifrons, and Bathymodiolus septemdierum, and characterized three types of hemocytes: agranulocytes (AGs), basophilic granulocytes (BGs), and eosinophilic granulocytes (EGs). Of these, the EG cells were the largest (diameter, 8.4-10.0 μm) and had eosinophilic cytoplasm with numerous eosinophilic granules (diameter, 0.8-1.2 μm). Meanwhile, the BGs were of medium size (diameter, 6.7-8.0 μm) and contained small basophilic granules (diameter, 0.3-0.4 μm) in basophilic cytoplasm, and the AGs, the smallest of the hemocytes (diameter, 4.8-6.0 μm), had basophilic cytoplasm lacking granules. A lectin binding assay revealed that concanavalin A bound to all three hemocyte types, while wheat germ agglutinin bound exclusively to EGs and BGs. The total hemocyte population densities within the hemolymph of all three Bathymodiolus mussel species were similar (8.4-13.3 × 10(5) cells/mL), and the percentages of circulating AGs, BGs, and EGs in the hemolymph of these organisms were 44.7-48.5%, 14.3-17.6%, and 34.3-41.0%, respectively. To analyze the functional differences between these hemocytes, the phagocytic activity and post-phagocytic phagosome-lysosome fusion events were analyzed in each cell type using a fluorescent Alexa Fluor(®) 488-conjugated Escherichia coli bioparticle and a LysoTracker(®) lysosomal marker, respectively. While the AGs exhibited no phagocytic activity, both types of granulocytes were phagocytic. Of the three hemocyte types, the EGs exhibited the highest level of phagocytic activity as well as rapid phagosome-lysosome fusion, which occurred within 2 h of incubation. Meanwhile, the BGs showed lower phagocytic activity and lower rates of phagosome-lysosome fusion than the EGs. These findings indicate that the two types of granulocyte play distinct roles in the defense system.

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Section: Discussionsupporting

confidence: 39%

Phagocytic activities of hemocytes from the deep-sea symbiotic mussels Bathymodiolus japonicus, B. platifrons, and B. septemdierum

Tame

Yoshida

Ohishi

et al. 2015

Fish & Shellfish Immunology

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“…Hemocytes of marine bivalves possess various physiological functions, such as immune defense, detoxification, and tissue and shell repair (Ludovic et al, 2009). Cellular defense, which includes hemocytosis, phagocytosis, encapsulation, and nacrezation, eliminates the invading foreign particles through the use of enzymes and reactive oxygen species in the hemocytes (Wang et al, , 2012a. As filter feeders, mussels are often used as a bioindicators for marine pollution (Cajaraville et al, 2000), especially for testing nanoparticle toxicity, because mussel hemocytes possess well developed endocytosis and phagocytic mechanisms (Cajaraville and Pal, 1995;Canesi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…According to previous studies, the hemocyte is a key parameter in the activation of the immune system in mussels and thus one of the important targets for nanoparticle toxicity (Couleau et al, 2012). Flow cytometry has been used in bivalve immunology because it provides fast, accurate, and quantitative analyses of cell characteristics and immune-related functions (Park et al, 2012;Wang et al, 2012a). Therefore, we characterized the immune-related parameters of hemocytes for the first time, in vivo, in the mussel M. coruscus exposed to nano-TiO 2 under seawater acidification conditions for 14 d followed by a 7 d recovery period.…”

Section: Introductionmentioning

confidence: 99%

Hemocyte responses of the thick shell mussel Mytilus coruscus exposed to nano-TiO 2 and seawater acidification

et al. 2016

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“…The gill represents the main interface between aquatic organisms and the external environment. Bivalve mollusk gills are one of the first lines of defense against bacterial infection; it has become increasingly evident that the hemocytes play a central role in the internal defense of bivalves (Pipe, 1990;Bachere et al, 1991;Wang et al, 2012). The upregulation of Mm-LGBP mRNA after Vibrio anguillarum challenge showed that Mm-LGBP plays a pivotal role in antibacterial immunity, further confirming the presence of the LPS recognition and binding site in Mm-LGBP.…”

mentioning

confidence: 81%

Lipopolysaccharide and β-1,3-glucan binding protein in the hard clam (Meretrix meretrix): Molecular characterization and expression analysis

Liu¹,

Qi²,

Zhang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix (designated as Mm-LGBP) by the expressed sequence tags and rapid amplification of cDNA ends method. The cDNA was 1827 bp in length, consisting of a 71-bp 5'-terminal untranslated region, a 62-bp 3'UTR, and a 1734-bp open reading frame encoding a 577-amino acid polypeptide with an estimated molecular mass of 60.7 kDa and a theoretical isoelectric point of 5.56. Characteristic potential Mm-LGBP from Meretrix meretrixpolysaccharide binding, cell adhesion, and glucanase motifs were identified in the Mm-LGBP, indicating that Mm-LGBP should be a new member of the LGBP family. Quantitative real-time polymerase chain reaction was developed to detect the mRNA expression level of Mm-LGBP in 6 different tissues. Higher-level mRNA expression of Mm-LGBP was detected in the gill and digestive gland tissues. The upregulation of Mm-LGBP mRNA after Vibrio anguillarum challenge showed that Mm-LGBP play a pivotal role in antibacterial immunity.

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Characterization of subpopulations and immune-related parameters of hemocytes in the green-lipped mussel Perna viridis

Cited by 67 publications

References 56 publications

Phagocytic activities of hemocytes from the deep-sea symbiotic mussels Bathymodiolus japonicus, B. platifrons, and B. septemdierum

Phagocytic activities of hemocytes from the deep-sea symbiotic mussels Bathymodiolus japonicus, B. platifrons, and B. septemdierum

Hemocyte responses of the thick shell mussel Mytilus coruscus exposed to nano-TiO 2 and seawater acidification

Lipopolysaccharide and β-1,3-glucan binding protein in the hard clam (Meretrix meretrix): Molecular characterization and expression analysis

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