Characterization of subpopulations of lipoprotein particles isolated from human cerebrospinal fluid

Borghini, Isabelle; Barja, Francisco; Pometta, D; James, Richard

doi:10.1016/0005-2760(94)00232-n

Cited by 152 publications

(127 citation statements)

References 36 publications

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“…Lipids, especially lipoproteins, are involved in the regulation of neural functions in the CNS through local mechanisms that are linked to systemic lipid metabolism [19,20]. Under normal and physiological conditions, high concentrations of HDL and LDL are present in CNS, as a result of transport across the brain-blood barrier (BBB) [21]. However, dyslipidemia can potentiate inflammatory processes at the vascular endothelium, lead to the production of adhesion molecules and to the recruitment of leukocytes [22,23].…”

Section: Discussionmentioning

confidence: 99%

New Markers of Early Cardiovascular Risk in Multiple Sclerosis Patients: Oxidized-LDL Correlates with Clinical Staging

et al. 2013

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Abstract. OBJECTIVES:This study aimed to characterize a population of multiple sclerosis (MS) patients in terms of traditional and new cardiovascular risk factors and assess their putative correlation with clinical disease activity (evaluated by the Expanded Disability Status Scale [EDSS] 00 (28.25-40.25) years and scoring a median of 2.00 (1.50-3.13) in EDSS. Comparing with controls, the most relevant differences encountered were: increased serum triglycerides (P < 0.001), Ox-LDL (P < 0.001) as well as Ox-LDL/LDL ratio and reduced small HDL (P = 0.040), accompanied by a trend to increased VEGF concentration. LDL content, especially Ox-LDL, showed positive and significant correlation with EDSS (r = 0.458; P = 0.011) and VEGF (r = 0.453; P = 0.014). CONCLUSIONS: MS patients presented a profile of early CV risk, being Ox-LDL contents a putative good marker and having correlation with the clinical activity of the disease.

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Section: Discussionmentioning

confidence: 99%

New Markers of Early Cardiovascular Risk in Multiple Sclerosis Patients: Oxidized-LDL Correlates with Clinical Staging

et al. 2013

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“…Pitas et al (1987) used immunoaffinity columns to identify two populations of CSF lipoproteins: a fraction that contained only apoA-I and a fraction that contained predominantly apoE. Borghini et al (1995) likewise demonstrated fractions that contained predominantly apoE or predominantly apoA-I but also suggested that some lipoprotein particles contained both apolipoproteins.…”

Section: Discussionmentioning

confidence: 99%

“…Pitas et al (1987) suggested that apoE and apoA-I generally do not reside together on the same CSF lipoprotein particles, but instead reside primarily in distinct particle populations. Borghini et al (1995) presented partially conflicting data suggesting that some particles have both apolipoproteins but that substantial populations of CSF lipoproteins have apoE without apoA-I and apoA-I without apoE.…”

mentioning

confidence: 99%

Novel Large Apolipoprotein E‐Containing Lipoproteins of Density 1.006–1.060 g/ml in Human Cerebrospinal Fluid

Guyton

Miller

Martin³

et al. 1998

Journal of Neurochemistry

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Abstract:Although the critical role of apolipoprotein F (apoE) allelic variation in Alzheimer's disease and in the outcome of CNS injury is now recognized, the functions of apoE in the CNS remain obscure, particularly with regard to lipid metabolism. We used density gradient ultracentrifugation to identify apoE-containing lipoproteins in human CSF. CSF apoE lipoproteins, previously identified only in the 1.063-1.21 g/ml density range, were also demonstrated in the 1.006-1.060 g/ml density range. Plasma lipoproteins in this density range include lowdensity lipoprotein and high-density lipoprotein (HDL) subfraction 1 (HDL 1). The novel CSF àpoE lipoproteins are designated HDL1. No immunoreactive apolipoprotein A-I (apo A-I) or B could be identified in the CSF HDL1 fractions. Large lipoproteins 18.3 ±6.6 nm in diameter (mean ±SD) in the HDL1 density range were demonstrated by electron microscopy. Following fast protein liquid chromatography of CSF at physiologic ionic strength, apoE was demonstrated in particles of average size greater than particles containing apoA-I. The largest lipoproteins separated by this technique contained apoE without apoA-I. Thus, the presence of large apoE-contaming lipoproteins was confirmed without ultracentrifugation. Interconversion between the more abundant smaller apoE-HDL subfractions 2 and 3 and the novel larger apoE-HDL1 is postulated to mediate a role in cholesterol redistribution in brain.

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“…In the plasma, apoE is present in a variety of types of lipoproteins such as large very low-density lipoproteins (VLDL) as well as high-density lipoproteins (HDL). In the cerebrospinal fluid (CSF), apoE is only present in lipoproteins that are HDL-like in size (Borghini et al, 1995;LaDu et al, 1998;Pitas et al, 1987b;Roheim et al, 1979). Interestingly, apoE-containing lipoproteins in the CSF are produced predominantly, if not exclusively, by cells within the CNS (Linton et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-β

Morikawa

Fryer

Sullivan

et al. 2005

Neurobiology of Disease

122

127

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The apolipoprotein E (apoE) genotype is an important genetic risk factor for Alzheimer's disease (AD). In the central nervous system (CNS), most apoE is produced by astrocytes and is present in unique high-density lipoprotein (HDL)-like particles that have distinct properties from apoE derived from other sources. To develop an efficient system to produce astrocyte-derived apoE in large quantities, we produced and characterized immortalized cell lines from primary astrocyte cultures derived from human APOE knock-in mice. APOE2, APOE3, and APOE4 expressing cell lines were established that secrete apoE in HDL-like particles at similar levels, cholesterol composition, and size as those produced by primary astrocytes. In physiological buffers, astrocyte-secreted apoE3 and E4 associated equally well with amyloid-B. Under the same conditions, only a small fraction of AB formed sodium dodecyl sulfate (SDS)-stable complexes with apoE (E3 N E4). These immortalized astrocytes will be useful for studying mechanisms underlying the isoform-specific effects of apoE in the CNS. D 2004 Elsevier Inc. All rights reserved.

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Characterization of subpopulations of lipoprotein particles isolated from human cerebrospinal fluid

Cited by 152 publications

References 36 publications

New Markers of Early Cardiovascular Risk in Multiple Sclerosis Patients: Oxidized-LDL Correlates with Clinical Staging

New Markers of Early Cardiovascular Risk in Multiple Sclerosis Patients: Oxidized-LDL Correlates with Clinical Staging

Novel Large Apolipoprotein E‐Containing Lipoproteins of Density 1.006–1.060 g/ml in Human Cerebrospinal Fluid

Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-β

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