Characterization of sulfate-reducing bacteria isolated from oil-field waters

Tardy-Jacquenod, C.; Cartigny, Pierre; Matheron, Robert; Lanau, Colette; Arnauld, O.; Magot, Michel

doi:10.1139/m96-038

Cited by 60 publications

(20 citation statements)

References 22 publications

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“…Seven different fermentative strains were purified, and six of them were shown to reduce sodium thiosulfate to hydrogen sulfide (28), including the recently described novel species Haloanaerobium congolense (37). Two SRB strains (42) and two methanogens, including the new species Methanoplanus petrolearius (34), were also isolated. None of these isolates displayed characteristics common to the described species (34,37,42).…”

Section: Time (Days)mentioning

confidence: 99%

“…Two SRB strains (42) and two methanogens, including the new species Methanoplanus petrolearius (34), were also isolated. None of these isolates displayed characteristics common to the described species (34,37,42).…”

Section: Time (Days)mentioning

confidence: 99%

“…Besides different SRB species (42), several strains of non-sulfate-, thiosulfate-reducing anaerobic bacteria were isolated. We report here on the characterization of one of these strains that induced intense pitting corrosion of mild steel in the presence of thiosulfate.…”

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confidence: 99%

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Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a New Anaerobic, Slightly Halophilic, Thiosulfate-Reducing Bacterium from Corroding Offshore Oil Wells

Magot

Ravot

Campaignolle

et al. 1997

International Journal of Systematic Bacteriology

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145

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A strictly anaerobic thiosulfate-reducing bacterium was isolated from a corroding offshore oil well in Congo and was designated strain SEBR 4207T. Pure culture of the strain induced a very active pitting corrosion of mild steel, with penetration rates of up to 4 mm per year. This constitutes the first experimental evidence of the involvement of thiosulfate reduction in microbial corrosion of steel. Strain SEBR 4207T cells were vibrios (3 to 5 by 1 pm), stained gram negative, and possessed lateral flagella. Spores were not detected. Optimum growth occurred in the presence of 3% NaCl at pH 7.0 and 42°C. Strain SEBR 4207T utilized peptides and amino acids, but not sugars or fatty acids. It fermented serine, histidine, and Casamino Acids, whereas arginine, glutamate, leucine, isoleucine, alanine, valine, methionine, and asparagine were only used in the presence of thiosulfate. Peptides were fermented to acetate, isobutyrate, isovalerate, 2-methylbutyrate, H,, and CO,. The addition of either thiosulfate or sulfur but not sulfate increased peptide utilization, growth rate, and biomass; during growth, H,S was produced and a concomitant decrease in H, was observed. The addition of either thiosulfate or sulfur also reversed H, inhibition. 16s rRNA sequence analysis indicates that strain SEBR 4207T is distantly related to members of the genus Thermoanuerobacter (83% similarity). Because the phenotypic and phylogenetic characteristics cannot be assigned to any described genus, strain SEBR 4207T is designated as a new species of a new genus, Dethiosulfovibrio peptidovorans gen. nov., sp. nov. Strain SEBR 4207T has been deposited in the Deutsche Sammlung von Mikroorganismen und zellkulturen GmbH (= DSM 11002).In 1989, Elf Congo experienced corrosion of the first 5 km of a 23-km main subsea pipeline that transported sour oil (i.e., H,S-containing petroleum) produced from the Emeraude oil field. The corroded segment was replaced, but it corroded again a year later. The whole line was then replaced and operated under a specifically designed biocide treatment regimen. The preliminary examination of the corroded iron showed that the breakthrough was due to bacterial pitting corrosion, with an unusually high penetration rate of about 1 cm per year. Chemical analysis of the pipeline water revealed the presence of up to 0.5 mM thiosulfate. It is likely that the thiosulfate was produced as a result of oxidation of the H,S naturally present in the oil field ecosystem by oxygen that is introduced in the pipelines during processing (10, 15, 22). The corrosion of pipelines was suspected to be due not only to sulfate but also to thiosulfate reduction by sulfate-reducing bacteria (SRB), because computer modeling had shown that thiosulfate reduction could induce the pitting corrosion of steel at higher rates than sulfate reduction (13).Since the pipeline was under a biocide treatment regimen during our microbiological investigations, the production fluids from several wellheads upstream of the line were collected and analyzed. Besides diffe...

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Section: Time (Days)mentioning

confidence: 99%

Section: Time (Days)mentioning

confidence: 99%

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Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a New Anaerobic, Slightly Halophilic, Thiosulfate-Reducing Bacterium from Corroding Offshore Oil Wells

Magot

Ravot

Campaignolle

et al. 1997

International Journal of Systematic Bacteriology

Self Cite

145

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“…Previous studies recognized SRB as the principal causative organism responsible for corrosion on steel structures buried underground which cause unexpected failures (Cord-Ruwisch et al, 1987;Tardy-Jacquenod et al, 1996;Al-Jaroudi et al, 2011). This microorganism may expedite MIC in pipeline systems primarily due to their predominantly anaerobic lifestyle and continuous production of corrosive hydrogen sulphide (Javaherdashti, 2009;Al-Abbas et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Statistical Investigation on Anaerobic Sulphate-Reducing Bacteria Growth by Turbidity Method

Ismail

Noor²,

Yahaya³

et al. 2015

International J. of Biological Chemistry

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In oil and gas industry, corrosion due to activity of microorganism is one of the main factors, which contribute to catastrophic structural failure. Previous study always linked Sulfate-Reducing Bacteria (SRB) upon the mechanism of Microbiologically Influenced Corrosion (MIC), as the major contributors. In this study, mechanisms of SRB genus D. vulgaris in terms of bacterial growth under influence of environmental factors were investigated. The growth of pure strain ATCC 7757 and SRB isolated from the soil in suspected areas in Peninsular Malaysia were investigated by using turbidity measurement. Results from the study were analyzed statistically to show the significant influence due to various environmental factors. The results agreed that variation of each environmental parameter tested gives strong influence upon bacterial growth for SRB strain individually.

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“…In the course of a survey of different oilfields, we isolated several strains of sulfate-reducing bacteria (1,18,24,25), including the mesophilic spore-forming strain designated SEBR 3 139, an isolate from a French oilfield brine able to grow at salinities of about 14%…”

Section: Introductionmentioning

confidence: 99%

Desulfotomaculum halophilum sp. nov., a halophilic sulfate-reducing bacterium isolated from oil production facilities

Tardy-Jacquenod

Magot²,

Patel³

et al. 1998

International Journal of Systematic Bacteriology

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Characterization of sulfate-reducing bacteria isolated from oil-field waters

Cited by 60 publications

References 22 publications

Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a New Anaerobic, Slightly Halophilic, Thiosulfate-Reducing Bacterium from Corroding Offshore Oil Wells

Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a New Anaerobic, Slightly Halophilic, Thiosulfate-Reducing Bacterium from Corroding Offshore Oil Wells

Statistical Investigation on Anaerobic Sulphate-Reducing Bacteria Growth by Turbidity Method

Desulfotomaculum halophilum sp. nov., a halophilic sulfate-reducing bacterium isolated from oil production facilities

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