Desulfotomaculum halophilum sp. nov., a halophilic sulfate-reducing bacterium isolated from oil production facilities

Tardy-Jacquenod, C.; Magot, Michel; Patel, Bharat K. C.; Matheron, Robert; Cartigny, Pierre

doi:10.1099/00207713-48-2-333

Cited by 87 publications

(34 citation statements)

References 24 publications

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“…In many cases an indigenous nature of a detected organism can be excluded. This is the case for some mesophilic organisms detected in samples from high temperature oil wells (Magot et al 1992;Tardy-Jacquenod et al 1998). On the other hand, the widespread distribution of thermophilic organismse.g.…”

Section: Introductionmentioning

confidence: 90%

Microbial community structure analysis of produced water from a high-temperature North Sea oil-field

Dahle

Garshol

Madsen

et al. 2007

Antonie van Leeuwenhoek

177

133

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Molecular and culture-based methods were used to investigate the microbial diversity in produced water obtained from the high-temperature Troll oil formation in the North Sea. 16S rRNA gene libraries were generated from total community DNA, using universal archaeal or bacterial oligonucleotide primer sets. Sequence analysis of 88 clones in the bacterial library indicated that they originated from members of Firmicutes (48 sequences), Bacteroidetes (17 sequences), delta-Proteobacteria (15 sequences), Spirochaetes (5 sequences), Thermotogales (2 sequences) and gamma-Proteobacteria (1 sequence). Twenty-two sequences in the archaeal library were close relatives to members of the genera Methanococcus (18 sequences), Methanolobus (3 sequences) and Thermococcus (1 sequence). Most of the bacterial sequences shared less than 95% identity with their closest match in GenBank, indicating that the produced water harbours a unique community of novel bacterial species or genera. Members of the thermophilic genera Thermosipho, Thermotoga, Anaerophaga and Thermovirga were isolated. The Troll formations are not injected with sea water. Thus, dramatic changes of the in situ conditions have been avoided, and a common source of continuous contamination from injection water can be excluded. However, the majority of the organisms detected in the gene libraries were most closely related to cultivated organisms with optimum temperatures for growth well below the in situ reservoir temperature (70 degrees C), indicating that produced water from the Troll platform harbours a substantial amount of non-indigenous organisms. This was confirmed by the isolation of a number of mesophilic and moderately thermophilic organisms that were unable to grow at reservoir temperature.

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Section: Introductionmentioning

confidence: 90%

Microbial community structure analysis of produced water from a high-temperature North Sea oil-field

Dahle

Garshol

Madsen

et al. 2007

Antonie van Leeuwenhoek

177

133

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“…Sequences close to Desulfococcus, Desulfomicrobium, Desulfobulbus, Desulfotignum, Desulfonatronovibrio, and Desulfonauticus were also detected. A wide range of Desulfovibrionaceae, Desulfobacteraceae, and Desulfotomaculum-related sequences have previously been found from oil field samples (34)(35)(36)(37)(38). Archaeoglobus fulgidus-like sequences were found from samples MOB1 and MOB9, which were both from the North Sea.…”

Section: Discussionmentioning

confidence: 99%

Application of Denaturing High-Performance Liquid Chromatography for Monitoring Sulfate-Reducing Bacteria in Oil Fields

Priha

Nyyssönen

Bomberg

et al. 2013

Appl Environ Microbiol

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Sulfate-reducing bacteria (SRB) participate in microbially induced corrosion (MIC) of equipment and H 2 S-driven reservoir souring in oil field sites. Successful management of industrial processes requires methods that allow robust monitoring of microbial communities. This study investigated the applicability of denaturing high-performance liquid chromatography (DHPLC) targeting the dissimilatory sulfite reductase ß-subunit (dsrB) gene for monitoring SRB communities in oil field samples from the North Sea, the United States, and Brazil. Fifteen of the 28 screened samples gave a positive result in real-time PCR assays, containing 9 ؋ 10 1 to 6 ؋ 10 5 dsrB gene copies ml ؊1 . DHPLC and denaturing gradient gel electrophoresis (DGGE) community profiles of the PCR-positive samples shared an overall similarity; both methods revealed the same samples to have the lowest and highest diversity. The SRB communities were diverse, and different dsrB compositions were detected at different geographical locations. The identified dsrB gene sequences belonged to several phylogenetic groups, such as Desulfovibrio, Desulfococcus, Desulfomicrobium, Desulfobulbus, Desulfotignum, Desulfonatronovibrio, and Desulfonauticus. DHPLC showed an advantage over DGGE in that the community profiles were very reproducible from run to run, and the resolved gene fragments could be collected using an automated fraction collector and sequenced without a further purification step. DGGE, on the other hand, included casting of gradient gels, and several rounds of rerunning, excising, and reamplification of bands were needed for successful sequencing. In summary, DHPLC proved to be a suitable tool for routine monitoring of the diversity of SRB communities in oil field samples.

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“…These taxonomic status of the Desulfotomaculum guttoideum is unclear, this strain shows high sequence similarity to a previously described Clostridium species (Stackebrandt et al, 1997). Since the phylogenetic survey of the genus was presented (Stackebrandt et al, 1997), four additional species have been added to the genus : Desulfotomaculum putei (Liu et al, 1997), Desulfotornaculum luciae (Liu et al, 1997), ' Desulfotomaculurn auripigrnentum' (Newman et al, 1997) and Desdfotomaculum halophilum (Tardy-Jacquenod et al, 1998). With the exception of ' Desulfotomaculum auripigmentum', which shows highest sequence similarity to the genus Desulfosporosinus, these newly described species add further phylogenetic diversity to the genus Desulfotomaculum. Members of these genera seem to be well adapted to changing redox conditions, facilitated by the formation of spores.…”

Section: Introductionmentioning

confidence: 97%

“…With the exception of ' Desulfotomaculum auripigmentum', which shows highest sequence similarity to the genus Desulfosporosinus, these newly described species add further phylogenetic diversity to the genus Desulfotomaculum. Members of these genera seem to be well adapted to changing redox conditions, facilitated by the formation of spores. They can be found in marine and freshwater ecosystems, including thermally heated groundwaters and oilfield brines, and were even isolated from corroded aluminium alloys (Widdel & Pfennig, 1981 ;Cord-Ruwisch & Garcia, 1985;Daumas et al, 1988;Nazina et al, 1989;Tardy-Jacquenod et al, 1998). The increase of new descriptions of sporeforming, sulfate-reducing bacteria in the last few years demonstrates the abundance and ecological import-01136 0 1999 IUMS ance of this specialized group (Min & Zinder, 1990;Tasaki et al, 1991;Kuever et al, 1993;Vainshtein et al, 1995;Liu et al, 1997;Love et al, 1993;Nilsen et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Description of Desulfotomaculum sp. Groll as Desulfotomaculum gibsoniae sp. nov.

Kuever

Rainey

Hippe

1999

International Journal of Systematic and Evolutionary Microbiology

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Strain GrollT, isolated from fresh water, is a mesophilic, spore-forming, sulfatereducing bacterium that uses a large variety of substrates as electron donors ranging from simple organic compounds to long-chain fatty acids and several aromatic compounds. Sulfate, thiosulfate and sulf ite are used as electron acceptors. Homoacetogenic growth occurs under sulfate-free conditions. Substrate oxidation is usually complete, leading to CO,, but acetate or other fatty acids can accumulate at high substrate concentrations. The G+C content of the DNA is 5 4 8 mol%. Strain GrollT was found to be phenotypically and phylogenetically different from known members of the genus Desulfotomaculum. 165 rRNA gene sequence analyses show that this organism falls within the radiation of the genus Desulfotomaculum cluster and has < 96 YO sequence similarity to previously described species. The name Desulfotomaculum gibsoniae sp. nov. is proposed for this strain; the type strain is GrolIT (= DSM 72133.

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Desulfotomaculum halophilum sp. nov., a halophilic sulfate-reducing bacterium isolated from oil production facilities

Cited by 87 publications

References 24 publications

Microbial community structure analysis of produced water from a high-temperature North Sea oil-field

Microbial community structure analysis of produced water from a high-temperature North Sea oil-field

Application of Denaturing High-Performance Liquid Chromatography for Monitoring Sulfate-Reducing Bacteria in Oil Fields

Description of Desulfotomaculum sp. Groll as Desulfotomaculum gibsoniae sp. nov.

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