Characterization of surface layer proteins and its role in probiotic properties of three Lactobacillus strains

Meng, Jianghong; Zhu, Xiao Dong; Gao, Shuming; Zhang, Qiuxiang; Sun, Zilong; Lu, Rongrong

doi:10.1016/j.ijbiomac.2014.01.024

Cited by 43 publications

(30 citation statements)

References 32 publications

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“…It is assumed that bacterial slp genes could be preferentially expressed under unfavourable conditions, forming a mechanical protein barrier which prevents the cells from being directly exposed to their environment (Gerbino, Carasi, Mobili, Seradell, & Gόmez-Zavaglin, 2015). The protective role of Slayer, discerned against adverse GI conditions and during lyophilisation in our study, was also observed by other authors (Frece, et al, 2005;Meng, et al, 2014;Uroić, et al, 2016).…”

Section: Accepted Manuscriptsupporting

confidence: 84%

Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Banić

Uroić

Pavunc

et al. 2018

LWT

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S-layers represent the simplest biological membranes developed during the evolution and are one of the most abundant biopolymers on Earth. Current fundamental and applied research aim to reveal the chemical structure, morphogenesis and function of S-layer proteins (Slps). This is the first paper that describes the Slps of certain Lactobacillus brevis strain isolated from sauerkraut. The whole genome sequence (WGS) analysis of the L. brevis SF9B strain uncovered three genes encoding the putative Slps, but merely one, identified as similar to the SlpB of L. brevis ATCC 14869, was expressed. Slp-expressing SF9B cells exhibited increased survival in simulated gastrointestinal

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Section: Accepted Manuscriptsupporting

confidence: 84%

Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Banić

Uroić

Pavunc

et al. 2018

LWT

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“…This was expected as bacterial adhesion to cellular surfaces is multifactorial and involves different specific and non-specific interactions between bacterial and cell surface components. Non-specific mechanisms involve electrostatic and hydrophobic interactions, whereas the specific mechanisms are related to surface molecules, such as outer membrane proteins (Frece et al, 2005; Chen et al, 2007; Johnson-Henry et al, 2007; Zhang et al, 2013; Meng et al, 2014), exopolysaccharides (EPSs, Lebeer et al, 2011), lipoteichoic acids (Granato et al, 1999), peptidoglycans (Van Tassell and Miller, 2011), glycosaminoglycans (Martin et al, 2013), flagella, and fimbriae (Singh et al, 2013). This complex scenario results in species-specific adhesion (Deepika and Charalampopoulos, 2010; Messaoudi et al, 2012; Verdenelli et al, 2014) and may be related to the differences observed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.

et al. 2016

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Lactobacilli are involved in the microbial homeostasis in the female genital tract. Due to the high prevalence of many bacterial diseases of the female genital tract and the resistance of microorganisms to various antimicrobial agents, alternative means to control these infections are necessary. Thus, this study aimed to evaluate the probiotic properties of well-characterized Lactobacillus species, including L. acidophilus (ATCC 4356), L. brevis (ATCC 367), L. delbrueckii ssp. delbrueckii (ATCC 9645), L. fermentum (ATCC 23271), L. paracasei (ATCC 335), L. plantarum (ATCC 8014), and L. rhamnosus (ATCC 9595), against Candida albicans (ATCC 18804), Neisseria gonorrhoeae (ATCC 9826), and Streptococcus agalactiae (ATCC 13813). The probiotic potential was investigated by using the following criteria: (i) adhesion to host epithelial cells and mucus, (ii) biofilm formation, (iii) co-aggregation with bacterial pathogens, (iv) inhibition of pathogen adhesion to mucus and HeLa cells, and (v) antimicrobial activity. Tested lactobacilli adhered to mucin, co-aggregated with all genital microorganisms, and displayed antimicrobial activity. With the exception of L. acidophilus and L. paracasei, they adhered to HeLa cells. However, only L. fermentum produced a moderate biofilm and a higher level of co-aggregation and mucin binding. The displacement assay demonstrated that all Lactobacillus strains inhibit C. albicans binding to mucin (p < 0.001), likely due to the production of substances with antimicrobial activity. Clinical isolates belonging to the most common Candida species associated to vaginal candidiasis were inhibited by L. fermentum. Collectively, our data suggest that L. fermentum ATCC 23271 is a potential probiotic candidate, particularly to complement candidiasis treatment, since presented with the best probiotic profile in comparison with the other tested lactobacilli strains.

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“…Meng et al (2014) reported that after removing SLPs from three strains of L. acidophilus and L. helveticus the survival in simulated gastric and intestinal juices was reduced by 2-3 log as compared with survival of the intact cells.…”

Section: Resistance To Gastrointestinal Conditionsmentioning

confidence: 99%

Role of S-layer proteins in bacteria

Gerbino

Carasi

Mobili

et al. 2015

World J Microbiol Biotechnol

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S-layers are paracrystalline bidimensional arrays of proteins or glycoproteins that overlay the cell surface of several genus and species of bacteria and archaea. As the outermost layer of several genus and species of microorganisms, S-layer proteins (SLP) are in direct contact with bacterial environment and thus may be involved in many of their surface properties, including adherence to various substrates, mucins and eukaryotic cells, aggregation and coaggregation with yeasts and other bacteria. In addition, SLP have been reported to be responsible for the bacterial protection against detrimental environmental conditions and to play an important role in surface recognition or as carriers of virulence factors. In this mini-review, we bring together the latest evidences about functional and mechanical properties of bacterial SLP from two different perspectives: (A) their role on bacterial adherence to different substrates and surfaces, and (B) their role as mechanical barriers in bacterial harmful environments.

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Characterization of surface layer proteins and its role in probiotic properties of three Lactobacillus strains

Cited by 43 publications

References 32 publications

Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.

Role of S-layer proteins in bacteria

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