Characterization of the Active Component in Bee Pollen <I>Cistus ladaniferus</I> Extract in Stimulating Bone Calcification and in Inhibiting Bone Resorption <I>in Vitro</I>

Abstract

The bee pollen Cistus ladaniferus (C. ladaniferus) extract has an anabolic effect on bone metabolism. The effects of the fractionated extracts obtained from bee pollen on bone calcium content and osteoclast-like cell formation in vitro were investigated. Rat femoral-diaphyseal and -metaphyseal tissues were cultured for 48 hr in a medium containing either vehicle or a water-solubilized extract with the membrane fractions obtained from bee pollen. The active component of bee pollen in increasing calcium content in diaphyseal tissues was seen in the fraction of molecular weight (MW) of less than 1000, and it was not observed in fractions of greater than MW 1000. Culture with the fractionated bee pollen extract (25 or 50 µg/ml of medium) of less than MW 1000 caused a significant increase in calcium content in the diaphyseal or metaphyseal tissues. The parathyroid hormone (PTH; 10 -6 M)-induced decrease in diaphyseal calcium content was significantly prevented in the presence of the fractionated bee pollen extracts (10 µg/ml) of less than MW 1000 or greater than MW 1000. Mouse marrow cells were cultured for 7 days in a medium containing PTH (10 -6 M) in the presence or absence of the fractionated bee pollen extract (10 or 50 µg/ml). The PTH-induced increase in osteoclast-like cell formation was markedly suppressed in the presence of extracts of less than MW 1000 as compared with that in the presence of fractions of greater than MW 1000. The effects of the fractionated bee pollen extracts of less than MW 1000 in increasing diaphyseal calcium content and in inhibiting PTH-induced osteoclastic cell formation were significantly decreased upon heat treatment for 20 and 60 min at 80°C. This study demonstrates that the active component of bee pollen C. ladaniferus extract, which stimulates bone formation and inhibits osteoclastic bone resorption, is the fraction with MW less than 1000.

Effect of bee pollen on the immunity and tibia characteristics in broilers

“…According to Hamamoto et al (2006) and Yamaguchi et al (2007b), bee pollen has stimulatory effects on bone formation and inhibitory effects on bone resorption. When evaluating the effect of the dietary inclusion of bee pollen extracts on femoral components, Yamaguchi et al (2006) verified that there was an increase in calcium and DNA content, due the cellular proliferation, and of alkaline phosphatase in rats' femur with the administration of 5 and 10 mg/100 g body weight.…”

Anabolic Effects of Bee Pollen Cistus ladaniferus Extract in Osteoblastic MC3T3-E1 Cells In Vitro

“…The active component, which stimulates bone formation and inhibits bone resorption in vitro, is found to be in the extract fraction with less than MW 1000. 15) The extract has been demonstrated to inhibit osteoclastogenesis in mouse marrow culture in vitro. This study, moreover, demonstrates that the extract fraction with less than MW 1000 has anabolic effects on osteoblastic MC3T3-E1 cells in vitro.…”

“…The water-solubilized extract from bee pollen was purified using the membrane fractionation method with less than MW 1000. 15) Cell Culture --Osteoblastic MC3T3-E1 cells were cultured at 37 • C in a CO 2 incubator in plastic dishes containing α-MEM supplemented with 10% FBS. They were subcultured every 3 days using 0.2% trypsin plus 0.02% EDTA in Ca 2+ / Mg 2+ -free phosphate-buffered saline (PBS).…”

“…15) This study was undertaken to determine whether the cistus extract with the fraction of less than MW 1000 has anabolic effects on osteoblastic MC3T3-E1 cells, which involve in bone formation.…”

Preventive Effects of Bee Pollen Cistus ladaniferus Extract on Bone Loss in Ovariectomized Rats In Vivo

“…The active component of bee pollen cistus extract, which stimulates bone formation and inhibits osteoclastic bone resorption, has been shown to be a fraction with molecular weight (MW) of less than 1000. 19) The intake of bee pollen cistus extract has been shown to have preventive effects on bone loss in streptozotocin-diabetic rats, and the intake has partial restorative effects on serum biochemical findings with the diabetic state in vivo. 20) This study was undertaken to determine whether the intake of bee pollen cistus extract has preventive effects on bone loss induced in ovariectomized (OVX) rats, a model of osteoporosis, in vivo.…”