Characterization of the beta-adrenergic receptors of cultured human epidermal keratinocytes

Gazith, J.; Cavey, Marie T.; Cavey, Daniel; Shroot, Braham; Reichert, Uwe

doi:10.1016/0006-2952(83)90368-4

Cited by 29 publications

(11 citation statements)

References 19 publications

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“…This finding was confirmed by later studies that investigated binding affinities of selective agonists and antagonists [10][11][12], radioligand-binding assays of epidermal membrane fragments [3], and autoradiographic mapping of the beta adrenergic receptor subtypes on epidermal keratinocytes [4]. Since only the beta2AR is expressed in keratinocytes, this review will focus on this receptor subtype and its physiological role in the epidermis.…”

mentioning

confidence: 69%

“…One pathogenic mechanism advanced by the work of Schallreuter and colleagues implicates the beta2AR signaling pathway and catecholamine synthetic network within the epidermis (recently reviewed in [13]). Both keratinocytes and melanocytes express beta2AR [4,10,11,53], both cell types have the enzymatic machinery for catecholamine synthesis [53,54], and indeed, both have been demonstrated to generate norepinephrine (keratinocytes, but not melanocytes can generate epinephrine) [53][54][55]. Stimulation of the beta2AR on melanocytes results in an increase in intracellular levels of cAMP, and subsequently, increased melanogenesis [53].…”

Section: Vitiligo and Beta2armentioning

confidence: 99%

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Beta Adrenergic Receptors in Keratinocytes

Sivamani

Lam

Isseroff

2007

Dermatologic Clinics

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SynopsisBeta2 adrenergic receptors were identified in keratinocytes more than 30 years ago, but their function in the epidermis continues to be elucidated. Abnormalities in their expression, signaling pathway, or in the generation of endogenous catecholamine agonists by keratinocytes have been implicated in the pathogenesis of cutaneous diseases such as atopic dermatitis, vitiligo and psoriasis. New studies also indicate that the beta2AR also modulates keratinocyte migration, and thus can function to regulate wound re-epithelialization. This review focuses on the function of these receptors in keratinocytes and their contribution to cutaneous physiology and disease.Keywords beta-adrenergic; keratinocyte; atopic dermatitis; psoriasis; vitiligo; wound Physiology of the beta2 adrenergic receptor in keratinocytesOf the several identified classes of adrenergic receptors (alpha and beta, and their subtypes), it is of particular interest to note that human keratinocytes, the cells the make up the majority of the epidermis, express only beta2 adrenergic receptors (beta2AR) [1][2][3][4]. The beta adrenergic receptor is a classic seven transmembrane G protein coupled receptor. These receptors serve as the endogenous receptor to the catecholamine hormones norepinephrine and epinephrine, and can be further subdivided into beta1, beta2, and beta3 subtypes, based on their differential pharmacological response to catecholamines and specific antagonists, as well as differences in their protein sequences [5][6][7].The first studies to suggest the presence of betaAR in the epidermis were functional ones that demonstrated an increase in levels of cAMP in keratinocytes after stimulation with non-specific betaAR agonists [8,9]. Subsequently, work using agonists and antagonists with specificity for the different adrenergic receptor subtypes demonstrated that the increase in keratinocyte

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mentioning

confidence: 69%

Section: Vitiligo and Beta2armentioning

confidence: 99%

Beta Adrenergic Receptors in Keratinocytes

Sivamani

Lam

Isseroff

2007

Dermatologic Clinics

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“…The existence of β‐adrenergic innervation pathways has already been established in human skin 44 20 .…”

Section: Discussionmentioning

confidence: 99%

Innervation and receptor profiles of the human apocrine (epitrichial) sweat gland: routes for intervention in bromhidrosis

Lindsay

Holmes

Corbett

et al. 2008

British Journal of Dermatology

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“…To investigate whether the observed effect was due to direct action of NA, isoproterenol (0.01 mg/ g bw, (13)) was chosen as b-AR agonist for its high affinity and high specificity to keratinocyte b-ARs (25,26). It was administered on days 0 and 3 to mimic the acute effect of NA depletion as occurring with the guanethidine treatment.…”

Section: Neuro-pharmacological Manipulationsmentioning

confidence: 99%

Hair growth‐modulation by adrenergic drugs

Peters

Maurer

Botchkarev

et al. 1999

Experimental Dermatology

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Since we have recently shown that the β2‐adrenoreceptor (β2‐AR) expression of selected regions of the hair follicle (HF) epithelium as well as the number of adrenergic nerve fibers in murine skin change in a hair cycle‐dependent manner, this has raised the possibility that adrenergic nerves may exert “trophic” functions during HF cycling. To further explore this concept, we have investigated the effect of neuro‐pharmacological manipulations on hair growth (anagen) induction in quiescent telogen mouse skin in vivo. Here, we demonstrate that subcutaneous injections of the noradrenaline (NA)‐depleting agent guanethidine, or of the neurotoxin 6‐hydroxydopaine, but not of the β2‐AR agonist isoproterenol induce a premature onset of anagen in the lower back skin of C57BL/6 mice. On day 20 after the start of treatment, more than 80% of the guanethidine‐treated mice and ca. 65% of the 6‐hydroxydopamine‐treated (6‐OHDA) mice exhibited premature skin darkening and hair growth at the site of drug application, whereas less than one‐third of all control animals showed macroscopic signs of anagen developent. This was confirmed by histology, demonstrating mature anagen VI HFs only at the immediate site of treatment with guanethidine of 6‐OHDA as opposed to resting telogen HFs in the neighboring untreated skin area. This observation further supports the concept that sympathetic nerves are intimately involved in hair growth control and invites one to explore the neuro‐pharmacological manipulation of piloneural interactions as a novel therapeutic strategy for the management of hair growth disorders.

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Characterization of the beta-adrenergic receptors of cultured human epidermal keratinocytes

Cited by 29 publications

References 19 publications

Beta Adrenergic Receptors in Keratinocytes

Beta Adrenergic Receptors in Keratinocytes

Innervation and receptor profiles of the human apocrine (epitrichial) sweat gland: routes for intervention in bromhidrosis

Hair growth‐modulation by adrenergic drugs

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