Characterization of the biomechanical properties of canine trachea using a customized 3D-printed apparatus

Lee, Jennifer Sang jee; Park, Jong‐Hyun; Shin, Dong Ah; Ryu, Young Jin; Kim, Hee Chan; Lee, Jung Chan; Kwon, Seong Keun

doi:10.1016/j.anl.2018.10.010

Cited by 17 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tensile characteristics of animal tracheas have not previously been investigated thoroughly and many reported studies have employed homogenized rather than intact tracheas (18,19,23). Here we observed that the tensile characteristics (elastic modulus and tensile strength) of animal tracheas were positively correlated with the number of tracheal cartilage rings (Figure 3A,B,E,F).…”

Section: Discussionsupporting

confidence: 49%

Biomechanical strength dependence on mammalian airway length

Huang¹,

Wang²,

Zhang³

et al. 2021

J Thorac Dis

View full text Add to dashboard Cite

Background: The trachea is the uppermost respiratory airway element connecting the larynx to the bronchi Airway reconstructions in humans are often developed from animal models but there is limited knowledge comparing tracheal biomechanics between species. We aimed to assess the structure and biomechanics of porcine, canine, caprine and human airways. Methods: Tracheas from pigs (n=15), goats (n=9) and canines (n=9) were divided into three groups (4, 6 and 8-ringswhile human left principal brochi (n=12) were divided into two groups (3and-rings). Airway structures were compared using histology and scanning electron microscopy. Biomechanical properties were measured subjecting samples to uniaxial tension and compression, recording the elastic modulus and (tensile and compressive) strengths. Results: The structures of animal tracheal and human bronchia appeared similar. Biomechanical testing revealed that the elastic modulus of 8-ring tracheas was 1,190.48±363.68, 2,572.00±608.19 and 1,771.27±145.54 kPa, for porcine, canine and caprine samples, respectively, while corresponding tensile strengths were 437.63±191.41, 808.38±223.48 and 445.76±44.00 kPa. Comparable measures of anteriorposterior (A-P) compression strengths were 7.94±0.82, 7.54±0.07 and 8.10±1.87 N, respectively, whereas lateral compression strengths were 8.75±0.82, 14.55±2.29 and 11.12±0.40 N. Compression testing of human samples showed significant differences (P<0.05) between the 3-ring (A-P, 1.06±0.02 N; lateral, 0.55±0.06 N)and 5-ring groups (A-P, 1.08±0.64 N; lateral, 2.32±1.95 N). Conclusions: The tensile and compressive strengths of mammalian airways show positive correlations with the cartilage ring number (length). On the basis of structural and biomechanical comparisons, porcine, canine and caprine species appear suitable models for the study of airway reconstruction in human.

show abstract

Section: Discussionsupporting

confidence: 49%

Biomechanical strength dependence on mammalian airway length

Huang¹,

Wang²,

Zhang³

et al. 2021

J Thorac Dis

View full text Add to dashboard Cite

show abstract

“…[ 60 ] In a study, lidocaine was reported to increase the cough occurrence within 1 h after operation. [ 61 ] The visual analog scale (VAS) was used to assess sore throat of the patients in static and during cough (Figure 6d,e). Lowest pain scores were observed in the FBA group along with positive patient feedback.…”

Section: Resultsmentioning

confidence: 99%

Mucosa‐Like Conformal Hydrogel Coating for Aqueous Lubrication

et al. 2022

View full text Add to dashboard Cite

Mucosa is a protective and lubricating barrier in biological tissue, which has a great clinical inspiration because of its slippery, soft, and hydrophilic surface. However, mimicking mucosal traits on complex surface remains an enormous challenge. Herein, a novel approach to create mucosa‐like conformal hydrogel coating is developed. A thin conformal hydrogel layer mimicking the epithelial layer is obtained by first absorbing micelles, followed by forming covalent interlinks with the polymer substrate via interface‐initiated hydrogel polymerization. The resulting coating exhibits uniform thickness (≈15 µm), mucosa‐matched compliance (Young's modulus = 1.1 ± 0.1 kPa) and lubrication (coefficients of friction = 0.018 ± 0.003), robust interfacial bonding against peeling (peeling strength = 1218.0 ± 187.9 J m–2), as well as high water absorption capacity. It effectively resists adhesion of proteins and bacteria without compromising biocompatibility. As demonstrated by an in vivo cynomolgus monkey model and clinical trial, applications of the mucosa‐like conformal hydrogel coating on the endotracheal tube significantly reduce intubation‐related complications, such as invasive stimuli, mucosal lesions, laryngeal edema, inflammation, and postoperative pain. This work offers a promising prototype for surface decoration of biomedical devices and holds great prospects for clinical translation to enable interventional operations with minimally invasive impacts.

show abstract

“…In our previous study, we developed a novel cylindrical three-dimensional (3D) printed polycaprolactone (PCL) scaffold that has mechanical properties similar to the native rabbit trachea. [2][3][4][5][6][7] Although the implants retained proper structural integrity, many implants failed in our previous study due to inadequate epithelialization and plugging with mucus. 5 We speculated that the main cause of the failure of segmental tracheal reconstruction was insufficient blood supply, resulting in limitations in the transport of progenitor cells, nutrients, and oxygen for tracheal tissue regeneration.…”

Section: Introductionmentioning

confidence: 80%

Prevascularized Tracheal Scaffolds Using the Platysma Flap for Enhanced Tracheal Regeneration

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objectives: One of the greatest hurdles in tracheal tissue engineering is insufficient vascularization, which leads to delayed mucosal regeneration, inflammation, and restenosis. This study investigated whether a prevascularized segmental tracheal substitute using platysma can enhance tracheal mucosal regeneration.Methods: Three-dimensional (3D) printed scaffolds with (group M) or without (group S) Matrigel coating were implanted under the feeding vessels of the platysma in New Zealand White rabbits (n = 3) to induce vascularization. After 1 or 2 weeks, tracheal defects were created and vascularized scaffolds with feeders of the platysma were transplanted as rotational flaps. As controls, scaffolds with or without Matrigel coating was transplanted into a tracheal defect without prevascularization. Airway patency and epithelization were examined using a rigid bronchoscope every 2 weeks. Surviving animals were euthanized at 24 weeks, and microcomputed tomography and histological evaluation were performed.Results: Animals with 2 weeks of prevascularization showed longer survival than animals with 0 or 1 weeks of prevascularization regardless of the Matrigel coating. Wider airway patency was observed in group M than group S. Group M showed migration of epithelium over the scaffold from 4 weeks after transplantation and complete coverage with epithelium at 12 weeks, whereas group S showed migration of the epithelium from 14 weeks and incomplete coverage with epithelium even at 24 weeks.Conclusion: This two-step method, utilizing the platysma as an in vivo bioreactor, may be a promising approach to achieve long-term survival and enhanced luminal patency. Matrigel coating on the scaffold had a synergistic effect on epithelial regeneration.

show abstract

Characterization of the biomechanical properties of canine trachea using a customized 3D-printed apparatus

Cited by 17 publications

References 27 publications

Biomechanical strength dependence on mammalian airway length

Biomechanical strength dependence on mammalian airway length

Mucosa‐Like Conformal Hydrogel Coating for Aqueous Lubrication

Prevascularized Tracheal Scaffolds Using the Platysma Flap for Enhanced Tracheal Regeneration

Contact Info

Product

Resources

About