Characterization of the Citrobacter freundii phoE gene and development of C. freundii-specific oligonucleotides

Spierings, Gonnie; Ockhuijsen, Corrine; Hofstra, H.; Tommassen, Jan

doi:10.1016/0378-1097(92)90025-j

Cited by 2 publications

(3 citation statements)

References 28 publications

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“…Therefore, the Aha1 in this study is more likely a structural protein which functions as an adhesin. Sperandio et al [31] also found that the OmpU of V. cholerae, which is most likely an adherence factor but not a porin, does not possess this conserved PEFXG motif.…”

Section: Characterisation Of Aha1 Genementioning

confidence: 92%

“…Database comparisons showed that it has homology with porins ompN [28], PhoE [29] and ompF [30] from E. coli (28%, 28% and 26% identities, respectively), ompU from V. cholerae [31] (27% identities) and PhoE from Citrobacter freundii [32] (27% identities). It has some features in common with other porins including an acidic pI (4.37), lack of cysteine residues, a predicted structure with 16 beta sheets, and a hydrophobic carboxy-terminal sequence ending with Phe which has been proposed to be crucial for the localisation of porins in OM [26].…”

Section: Characterisation Of Aha1 Genementioning

confidence: 99%

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Cloning, characterisation and expression of Aeromonas hydrophila major adhesin

Fang

Sin

2004

Fish & Shellfish Immunology

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Section: Characterisation Of Aha1 Genementioning

confidence: 92%

Section: Characterisation Of Aha1 Genementioning

confidence: 99%

Cloning, characterisation and expression of Aeromonas hydrophila major adhesin

Fang

Sin

2004

Fish & Shellfish Immunology

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“…The enterobacterial outer membrane proteins cross the membrane several times and therefore exist of membrane-spanning conserved regions intermitted by exposed variable regions. The sophisticated use of selected nucleotide sequences from the variable regions created the opportunity to develop specific oligonucleotide probes for bacteria from the family Enterobacteriaceae [20][21][22][23].…”

Section: Microbial Identificationmentioning

confidence: 99%

Microbes in food processing technology

Hofstra

Vossen

Plas

1994

FEMS Microbiology Reviews

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There is an increasing understanding that the microbial quality of a certain food is the result of a chain of events. It is clear that the microbial safety of food can only be guaranteed when the overall processing, including the production of raw materials, distribution and handling by the consumer are taken into consideration. Therefore, the microbiological quality assurance of foods is not only a matter of control, but also of a careful design of the total process chain. Food industry has now generally adapted quality assurance systems and is implementing the Hazard Analysis Critical Control Point (HACCP) concept. Rapid microbiological monitoring systems should be used in these cases. There is a need for rapid and simple microbiological tests which can be adapted to the technology and logistics of specific production processes. Traditional microbiological methods generally do not meet these high requirements. This paper discusses the tests, based on molecular biological principles, to detect and identify microbes in food-processing chains. Tests based on DNA technology are discussed, including in vitro DNA amplification like the polymerase chain reaction (PCR) method and identifications based on RFLP, RAPD and DNA fingerprinting analysis. PCR-based methodology can be used for the rapid detection of microbes in food manufacturing environments. In addition, DNA fingerprinting methods are suitable for investigating sources and routes of microbial contamination in the food cycle.

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Characterization of the Citrobacter freundii phoE gene and development of C. freundii-specific oligonucleotides

Cited by 2 publications

References 28 publications

Cloning, characterisation and expression of Aeromonas hydrophila major adhesin

Cloning, characterisation and expression of Aeromonas hydrophila major adhesin

Microbes in food processing technology

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