Characterization of the complete mitochondrial genome of <i>Setaria digitata</i> (Nematoda: Setariidae) from China

Liu, G.-H.; Li, J.-Y.; Zhu, Xing‐Quan

doi:10.1017/s0022149x16000912

Cited by 12 publications

(8 citation statements)

References 23 publications

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“…Gel electrophoresis produced a 209 bp band, and the sample was confirmed as S. digitata when compared against other sequences in the NCBI GenBank database using BLAST. The genetic sequence extracted from the worm was 99% similar to that of S. digitata isolated from cattle and buffalos in Sri Lanka and from buffalos in China [20,21]. The mind-point rooted phylogenetic tree further confirmed that the S. digitata isolated from this study belongs to the same major clade isolated from Japan, India and Sri Lanka.…”

Section: Resultssupporting

confidence: 69%

“…Therefore, this finding suggests that cattle in northern China may carry S. digitata, and further investigation of its prevalence will be worthwhile. In 2017, S. digitata was first identified by molecular method in the cadavers of buffalos in southern China, yet not in live buffalos or cattle [21]. Setariasis is a well-recognized ophthalmological disorder in equine, with S. digitata being reported as the most common cause [27].…”

Section: Resultsmentioning

confidence: 99%

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First Molecular Confirmation of Equine Ocular Setaria digitata in China

Feng

Liu

Chen

et al. 2021

Veterinary Sciences

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A 5-year-old Mongolian mare (Equus caballus Linnaeus, 1758) was observed to have corneal opacity and excessive ocular discharge. An ophthalmic examination revealed a moving thread-like cylindrical worm in the anterior chamber of the right eye. The parasite was successfully removed surgically. The worm was observed under light microscopy and confirmed as Setaria digitata by 12S rRNA gene amplification and sequencing. Phylogenetic analysis demonstrated similarity with Setaria digitata in the National Center for Biotechnology Information (NCBI) GenBank database isolated from other Asian countries. This report is the first confirmed case of equine ocular setariasis by molecular diagnosis in China, which may indicate its presence in livestock and promote research on its epidemiology.

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

First Molecular Confirmation of Equine Ocular Setaria digitata in China

Feng

Liu

Chen

et al. 2021

Veterinary Sciences

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“…The analyses of several hundred mosquitoes collected in Cambodia, Mali, Guinea and Maryland revealed well-known human pathogens including P. falciparum, which was the target of the initial study of the Cambodian samples (24). In addition, we detected Theileria species and Setaria digitata, which cause livestock diseases in Southeast Asia (38)(39)(40)(41)(42). While we were initially unable to conclusively determine the exact Theileria species with our initial assay, targeted follow-up studies using longer amplicons and Sanger sequencing (Figure 2) revealed that the sequences amplified from the African mosquitoes were most closely related to T. velifera and T. mutans, which are both known to infect African cattle (43), whereas the Cambodian mosquitoes carried sequences most closely related to T. sinensis, a species that infects cattle in China (44).…”

Section: Discussionmentioning

confidence: 94%

High-throughput Identification of Eukaryotic Parasites and Arboviruses in Mosquitoes

Cannon

Bogale

Bhalerao

et al. 2021

Preprint

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Vector-borne pathogens cause many human infectious diseases and are responsible for high mortality and morbidity throughout the world. They can also cause livestock epidemics with dramatic social and economic consequences. Due to the high costs, vector-borne disease surveillance is often limited to current threats, and the investigation of emerging pathogens typically occur after the reports of clinical cases. Here, we use high-throughput sequencing to detect and identify a wide range of parasites and viruses carried by mosquitoes from Cambodia, Guinea, Mali and Maryland. We apply this approach to individual Anopheles mosquitoes as well as pools of mosquitoes captured in traps; and compare the outcomes of this assay when applied to DNA or RNA. We identified known human and animal pathogens and mosquito parasites belonging to a wide range of taxa, insect Flaviviruses, and novel DNA sequences from previously uncharacterized organisms. Our results also revealed that analysis of the content of an entire trap is an efficient approach to monitor and identify potential vector-borne pathogens in large surveillance studies, and that analyses of RNA extracted from mosquitoes is preferable, when possible, over DNA-based analyses. Overall, we describe a flexible and easy-to-customize assay that can provide important information for vector-borne disease surveillance and research studies to efficiently complement current approaches.

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Section: Biology Open • Accepted Manuscriptmentioning

confidence: 94%

High-throughput detection of eukaryotic parasites and arboviruses in mosquitoes

et al. 2021

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Vector-borne pathogens cause many human infectious diseases and are responsible for high mortality and morbidity throughout the world. They can also cause livestock epidemics with dramatic social and economic consequences. Due to its high costs, vector-borne disease surveillance is often limited to current threats, and the investigation of emerging pathogens typically occurs after the reports of clinical cases. Here, we use high-throughput sequencing to detect and identify a wide range of parasites and viruses carried by mosquitoes from Cambodia, Guinea, Mali and Maryland. We apply this approach to individual Anopheles mosquitoes as well as pools of mosquitoes captured in traps; and compare the outcomes of this assay when applied to DNA or RNA. We identified known human and animal pathogens and mosquito parasites belonging to a wide range of taxa, as well as novel DNA sequences from previously uncharacterized organisms. Our results also revealed that analysis of the content of an entire trap could be an efficient approach to monitor and identify rare vector-borne pathogens in large surveillance studies. Overall, we describe a high-throughput and easy-to-customize assay to screen for a wide-range of pathogens and efficiently complement current vector-borne disease surveillance approaches.

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Characterization of the complete mitochondrial genome of Setaria digitata (Nematoda: Setariidae) from China

Cited by 12 publications

References 23 publications

First Molecular Confirmation of Equine Ocular Setaria digitata in China

First Molecular Confirmation of Equine Ocular Setaria digitata in China

High-throughput Identification of Eukaryotic Parasites and Arboviruses in Mosquitoes

High-throughput detection of eukaryotic parasites and arboviruses in mosquitoes

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