Characterization of the cutaneous mycobiota in healthy and allergic cats using next generation sequencing

Meason-Smith, Courtney; Diesel, Alison; Patterson, Adam P.; Older, Caitlin E.; Johnson, Timothy J.; Mansell, Joanne; Suchodolski, Jan S.; Hoffmann, Aline Rodrigues

doi:10.1111/vde.12373

Cited by 67 publications

(76 citation statements)

References 39 publications

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“…Meason-Smith et al found that dogs most clustered by individual, regardless of health condition [21]. This same clustering by individual, regardless of health, was also seen in a similar study done on cats [20]. In both studies, differences in relative taxa abundance between healthy and allergic animals were identified.…”

Section: Discussionsupporting

confidence: 52%

“…In both studies, differences in relative taxa abundance between healthy and allergic animals were identified. Overall, it seems that fungi on the skin of cats [20] and dogs [21] clusters mostly by individual animal, whereas bacteria seem to cluster mostly by body site [9]. …”

Section: Discussionmentioning

confidence: 99%

“…Previously, a culture based study was performed with the goal of describing the feline skin microbiota and found that Micrococcus , Acinetobacter , Streptococci , and Staphylococci dominated the sites sampled; however, bacteria could not be isolated from half of the samples, which the authors attributed to the normal grooming behavior of cats [22]. Recent studies have also examined the fungal mycobiota in healthy and allergic cats and dogs, which have found significant differences in the fungal communities colonizing the skin [20, 21]. …”

Section: Introductionmentioning

confidence: 99%

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The feline skin microbiota: The bacteria inhabiting the skin of healthy and allergic cats

et al. 2017

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BackgroundThe skin is inhabited by a multitude of microorganisms. An imbalance of these microorganisms is associated with disease, however, the causal relationship between skin microbiota and disease remains unknown. To describe the cutaneous bacterial microbiota of cats and determine whether bacterial dysbiosis occurs on the skin of allergic cats, the skin surfaces on various regions of 11 healthy cats and 10 allergic cats were sampled.Methodology/Principal findingsGenomic DNA was extracted from skin swabs and sequenced using primers that target the V4 region of the bacterial 16S rRNA. The bacterial sequences from healthy cats revealed that there are differences in species diversity and richness between body sites and different epithelial surfaces. Bacterial communities preferred body site niches in the healthy cats, however, the bacterial communities on allergic cat skin tended to be more unique to the individual cat. Overall, the number of bacterial species was not significantly different between the two health status groups, however, the abundances of these bacterial species were different between healthy and allergic skin. Staphylococcus, in addition to other taxa, was more abundant on allergic skin.Conclusions/SignificanceThis study reveals that there are more bacterial species inhabiting the skin of cats than previously thought and provide some evidence of an association between dysbiosis and skin disease.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The feline skin microbiota: The bacteria inhabiting the skin of healthy and allergic cats

et al. 2017

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“…However, faecal antigen tests detect both field and vaccine strains of FPV and CPV. In one study evaluating three commercial CPV faecal antigen kits, 20% of cats vaccinated against FPV in the preceding 14 days returned positive test results [29].…”

Section: Discussionmentioning

confidence: 99%

Distinct Lineages of Feline Parvovirus Associated with Epizootic Outbreaks in Australia, New Zealand and the United Arab Emirates

Brussel

Carrai

Lin

et al. 2019

Viruses

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Feline panleukopenia (FPL), a frequently fatal disease of cats, is caused by feline parvovirus (FPV) or canine parvovirus (CPV). We investigated simultaneous outbreaks of FPL between 2014 and 2018 in Australia, New Zealand and the United Arab Emirates (UAE) where FPL outbreaks had not been reported for several decades. Case data from 989 cats and clinical samples from additional 113 cats were obtained to determine the cause of the outbreaks and epidemiological factors involved. Most cats with FPL were shelter-housed, 9 to 10 weeks old at diagnosis, unvaccinated, had not completed a primary vaccination series or had received vaccinations noncompliant with current guidelines. Analysis of parvoviral VP2 sequence data confirmed that all FPL cases were caused by FPV and not CPV. Phylogenetic analysis revealed that each of these outbreaks was caused by a distinct FPV, with two virus lineages present in eastern Australia and virus movement between different geographical locations. Viruses from the UAE outbreak formed a lineage of unknown origin. FPV vaccine virus was detected in the New Zealand cases, highlighting the difficulty of distinguishing the co-incidental shedding of vaccine virus in vaccinated cats. Inadequate vaccination coverage in shelter-housed cats was a common factor in all outbreaks, likely precipitating the multiple re-emergence of infection events.

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“…It was hypothesized that the canine mycobiota shifts in disease states, lowering fungal diversity and allowing certain fungal populations to predominate in skin lesions. Furthermore, the skin of atopic dogs also is described to be associated with altered skin barrier, such as caused by fillagrin mutations, whereas similar alterations in skin barriers have not been described or confirmed in allergic cats, which might explain why no alterations were seen in diversity in the feline allergic skin disease …”

Section: Dysbiosis Of the Skin Microbiome Is Associated With Skin Dismentioning

confidence: 99%

The cutaneous ecosystem: the roles of the skin microbiome in health and its association with inflammatory skin conditions in humans and animals

Hoffmann

2017

Veterinary Dermatology

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Characterization of the cutaneous mycobiota in healthy and allergic cats using next generation sequencing

Cited by 67 publications

References 39 publications

The feline skin microbiota: The bacteria inhabiting the skin of healthy and allergic cats

The feline skin microbiota: The bacteria inhabiting the skin of healthy and allergic cats

Distinct Lineages of Feline Parvovirus Associated with Epizootic Outbreaks in Australia, New Zealand and the United Arab Emirates

The cutaneous ecosystem: the roles of the skin microbiome in health and its association with inflammatory skin conditions in humans and animals

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