Characterization of the endokinins: Human tachykinins with cardiovascular activity

Page, Nigel M.; Bell, N.; Gardiner, Sheila M.; Manyonda, Isaac; Brayley, K; Strange, Philip G.; Lowry, P. J.

doi:10.1073/pnas.0931458100

Cited by 145 publications

(166 citation statements)

References 29 publications

Supporting

Mentioning

161

Contrasting

Unclassified

Order By: Relevance

“…Endokinins A and B are also expressed outside of neuronal tissue with high levels in the adrenal gland. Page et al (35) determined that the hemodynamic response of rats to SP is closely mimicked by endokinins A and B. Together, these results led the authors to propose that hemokinin 1 and endokinins A and B are SP-like agonists that act in the periphery where SP is not present (35).…”

Section: Discussionmentioning

confidence: 93%

“…Page et al (35) determined that the hemodynamic response of rats to SP is closely mimicked by endokinins A and B. Together, these results led the authors to propose that hemokinin 1 and endokinins A and B are SP-like agonists that act in the periphery where SP is not present (35). It remains to be determined which NK-1R agonist is most relevant to adhesion formation.…”

Section: Discussionmentioning

confidence: 98%

“…All three functionally bind the NK-1R with affinity that is similar to but less than that of SP (35). Hemokinin 1 is expressed outside of neuronal tissue predominantly in immune cells (34), and Weinstock (57) has recently reported that hemokinin 1 is produced at sites of inflammation in the gut lumen.…”

Section: Discussionmentioning

confidence: 99%

“…SP belongs to the tachykinin family of peptides that also includes neurokinins A and B, hemokinin 1, and endokinins A and B (21,34,35). The broad range of biological effects associated with SP and the other tachykinins is mediated by means of binding to one of three neurokinin (NK) receptors [NK-1 receptor (NK-1R), NK-2 receptor, or NK-3 receptor] (36).…”

mentioning

confidence: 99%

“…The broad range of biological effects associated with SP and the other tachykinins is mediated by means of binding to one of three neurokinin (NK) receptors [NK-1 receptor (NK-1R), NK-2 receptor, or NK-3 receptor] (36). SP has the highest selectivity for the NK-1R (21); however, the other tachykinins are also capable of functionally binding to the NK-1R (21,34,35).…”

mentioning

confidence: 99%

See 4 more Smart Citations

A neurokinin 1 receptor antagonist decreases postoperative peritoneal adhesion formation and increases peritoneal fibrinolytic activity

Reed

Fruin

Gower

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Fibrous adhesions remain a major sequela of abdominal surgery. The proinflammatory peptide substance P (SP), known to participate in inflammatory events, may play a key role in adhesion formation. This hypothesis was tested by using an antagonist, CJ-12,255 (Pfizer), that blocks the binding of SP to the neurokinin 1 receptor (NK-1R). Adhesion formation was surgically induced in the peritoneum of rats receiving daily doses of the NK-1R antagonist (NK-1RA; 5.0 or 10.0 mg͞kg per day) or saline. On postoperative day 7, both the low and high doses of NK-1RA significantly (P < 0.05) reduced adhesion formation by 45% and 53%, respectively, compared with controls. Subsequently, the effect of NK-1RA administration on peritoneal fibrinolytic activity was investigated to determine a potential mechanism for SP action in the peritoneum. Samples were collected from nonoperated controls and from animals 24 h postsurgery that were administered either NK-1RA or saline. Fibrinolytic activity in peritoneal fluid was assayed by zymography, and expression of tissue plasminogen activator (tPA) and plasminogen activator inhibitor 1, both regulators of fibrinolytic activity, was assessed in peritoneal tissue and fluid by RT-PCR and bioassay, respectively. NK-1RA administration led to a marked (P < 0.05) increase in tPA mRNA levels in peritoneal tissue compared with nonoperated and saline-administered animals. Likewise, NK-1RA administration significantly (P < 0.05) increased tPA in the peritoneal fluid. These data suggest that activation of the NK-1R promotes peritoneal adhesion formation by limiting fibrinolytic activity in the postoperative peritoneum, thus enabling fibrinous adhesions to persist.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

A neurokinin 1 receptor antagonist decreases postoperative peritoneal adhesion formation and increases peritoneal fibrinolytic activity

Reed

Fruin

Gower

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

Biology of Peptides

Sewald

Jakubke

2009

Peptides: Chemistry and Biology

View full text Add to dashboard Cite

Membrane‐induced structure of novel human tachykinin hemokinin‐1 (hHK1)

Ganjiwale

Cowsik

2015

Biopolymers

View full text Add to dashboard Cite

PPT-C encoded hemokinin-1(hHK-1) of Homo sapiens (TGKASQFFGLM) is a structurally distinct neuropeptide among the tachykinin family that participate in the NK-1 receptor downstream signaling processes. Subsequently, signal transduction leads to execution of various effector functions which includes aging, immunological, and central nervous system (CNS) regulatory actions. However the conformational pattern of ligand receptor binding is unclear. The three-dimensional structure of the hemokinin-1 in aqueous and micellar environment has been studied by one and two-dimensional proton nuclear magnetic resonance (2D 1H-NMR spectroscopy) and distance geometry calculations. Data shows that hemokinin-1 was unstructured in aqueous environment; anionic detergent SDS induces α-helix formation. Proton NMR assignments have been carried out with the aid of correlation spectroscopy (gradient-COSY and TOCSY) and nuclear Overhauser effect spectroscopy (NOESY and ROESY) experiments. The inter proton distances and dihedral angle constraints obtained from the NMR data have been used in torsion angle dynamics algorithm for NMR applications (CYANA) to generate a family of structures, which have been refined using restrained energy minimization and dynamics. Helical conformation is observed from residue K3-M11. The conformational range of the peptide revealed by NMR studies has been analyzed in terms of characteristic secondary features. Observed conformational features have been compared to that of Substance P potent NK1 agonist. Thus the report provides a structural insight to study hHK-1-NK1 interaction that is essential for hHK1 based signaling events.

show abstract

Characterization of the endokinins: Human tachykinins with cardiovascular activity

Cited by 145 publications

References 29 publications

A neurokinin 1 receptor antagonist decreases postoperative peritoneal adhesion formation and increases peritoneal fibrinolytic activity

A neurokinin 1 receptor antagonist decreases postoperative peritoneal adhesion formation and increases peritoneal fibrinolytic activity

Biology of Peptides

Membrane‐induced structure of novel human tachykinin hemokinin‐1 (hHK1)

Contact Info

Product

Resources

About