Characterization of the endophytic antagonist pY11T-3-1 against bacterial soft rot of Pinellia ternata

Abstract

Aims: Biocontrol is an emerging trend aimed at reducing chemical input while increasing plant fitness, productivity and resistance to diseases in sustainable agriculture. An antagonist, pY11T‐3‐1, was herein characterized for potential applications against soil‐borne plant diseases. Methods and Results: In vitro antagonistic assays, the antagonist pY11T‐3‐1 was demonstrated able to obviously reduce the occurrence of the soft rot disease on Pinellia ternata, potato, pepper, tomato, cucumber and eggplant tubers or fruits, with higher prevention (90%) on P. ternata. It showed a broad antagonistic spectrum against 23 tested bacterial and fungal phytopathogens, which were distributed in 14 genus and 17 species. However, it inhibited only two of the seven bacterial nonpathogens. Phenotypic characterizations showed that the antagonist pY11T‐3‐1 was similar to Pseudomonas aeruginosa. Its major fatty acids were 18:1 w7c (22·17%), 16:0 (20·21%), 12:0 2OH (12·45%), 16:1w7c/15 iso2OH (10·95%) and 10:0 3OH (10·79%), which is a different profile from that of Ps. aeruginosa. The 16S rRNA and gyr B gene sequences shared 100 and 99% similarity with Ps. aeruginosa, respectively. The phylogenetic trees showed that it was clustered with Ps. aeruginosa. Conclusions: The antagonist pY11T‐3‐1 was characterized as Ps. aeruginosa with a unique fatty acid profile. Significance and Impact of the Study: With broad antagonistic spectrum and host selectivity, the antagonist pY11T‐3‐1 may provide a more environmental and economical alternative to the control of soil‐borne disease on P. ternata, which needs further investigation.

ISOLAMENTO, IDENTIFICAÇÃO E AVALIAÇÃO DAS ATIVIDADES ENZIMÁTICA E ANTIBACTERIANA DE MICRO-ORGANISMOS ENDOFÍTICOS DE Hyptis suaveolens (L.) Poit.

“…Analisando-se os resultados obtidos nessa triagem, observa-se que os isolados do gênero Pseudomonas se sobressaíram frente as demais quanto a bioatividade. Bacteriocinas de endofíticos desse gênero são amplamente exploradas para o biocontrole de fitopatógenos (RAMESH et al, 2009;DONG et al, 2010). MCCAUGHEY et al, (2014) recentemente comprovaram que os receptoresalvo dessas substâncias de natureza peptídica de Pseudomonas são lipopolissacarídeos geralmente contendo D-ramnose, especificidade que possibilitam que essas proteínas antibióticas tenham uma toxicidade seletiva de interessante aplicação não somente para o controle biológico de pragas microbianas como também no tratamento de infecções por cepas multirresistentes de P. aeruginosa, com um mínimo impacto ambiental.…”

Isolation and identification of a new biocontrol bacteria against Salvia miltiorrhiza root rot and optimization of culture conditions for antifungal substance production using response surface methodology

“…Although chemical control can effectively inhibit the occurrence of fungal diseases in the short term, the application of chemical agents greatly increases pesticide residues in the soil, pollutes the environment and enhances the disease resistance of pathogenic microorganisms [6]. In recent years, more and more studies have shown that biological control using endophytes is an important alternative to chemical control [7][8][9].…”

Microarray-based identification of conserved microRNAs from Pinellia ternata

“…Breit is a monocotyledonous perennial herbaceous plant widely used for thousands of years in China as a most important herb in traditional Chinese medicine (Dong et al, 2010). It belongs to the genus Araceae, which is native to Asia, but it also grows in parts of North America, spreads by rhizomes, with bulbils at the base or the bottom of the leaves (Kim et al, 2006) or on the underground tubers, which are infrequently in other Araceae plants.…”