Characterization of the enterovirus 71 P1 polyprotein expressed inPichia pastoras a candidate vaccine

Effect of difference between EV-A71 virus epidemic strain and “vaccine strain” on neutralizing antibody titer

“…36 Such high mutation rate allows EV-A71 to adapt rapidly to selection pressures, which select for beneficial mutations. 37 It can be inferred that the epitope of the C4 strain functional protein has changed in 16 years, 38 , 39 and the epitope on the surface protein may make the immune response anti-infective. The type has a certain degree of cross-neutralization.…”

Enterovirus A71 Vaccines

“…Han et al [60] expressed the modified EV-A71 polyprotein P1 in Pichia pastoris as a vaccine candidate. P1 protein induced persistent, high cross-neutralization antibodies for different EV-A71 subtypes in rabbits.…”

Achievements, challenges and prospects for the development of broadly protective multivalent vaccines and therapeutic antibodies against hand, foot and mouth disease

“…EV71 is the leading causative agent for HFMD. Until now, a number of approaches have been taken to develop vaccines against EV71, including inactivated whole-virus vaccines [7][8][9][10], attenuated live virus vaccines [11], viruslike particles (VLPs) [12][13][14][15][16][17] and other types of EV71 vaccine candidates [18][19][20][21][22][23][24][25][26][27] (Table 1).…”

“…There are also other types of EV71 vaccine candidates. The precursor of EV71 capsid proteins P1, expressed in P. pastoris, was shown to be immunogenic and capable of eliciting improved humoral and cellular immune responses while also providing good cross-protection with different EV71 strains compared with the heat-inactivated EV71 [18].…”