Characterization of the exopolysaccharide produced by a whey utilizing strain of Klebsiella oxytoca

Dlamini, A.M.; Peiris, Paul; Bavor, John; Kailasapathy, Kasipathy

doi:10.5897/ajb2007.000-2416

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…Gas chromatography revealed that the monosaccharide composition of the crude EPS consisted of rhamnose (Rha; 29.83%), glucose (Glc; 11.21%), galactose (Gal; 52.45%), and mannose (Man; 6.50%); thus, galactose and rhamnose are the major components. These data are in agreement with those found by Dlamini et al (2007) and Feng, Li, Chen (2009), who used different media cultures for the production of EPS from K. oxytoca.…”

Section: Resultssupporting

confidence: 93%

“…Some of the properties that contribute to their broad application and, consequently, the realization of extensive research in this area, includes easy production by bacterial fermentation and manipulation by recombinant DNA technology, the ability to retain water and form films, specific rheological behavior, and the potential to improve the immune response (Barbara et al, 2009;Dlamini et al, 2007;Dlamini et al, 2009;Snapper, 2016;Sun et al, 2016;Donot et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Exopolysaccharides from Klebsiella oxytoca: anti-inflammatory activity

Bannwart

Bersani-Amado

Ames

et al. 2022

Braz. J. Pharm. Sci.

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Exopolysaccharides (EPS) produced by Klebsiella oxytoca are of environmental, pharmaceutical, and medicinal interest. However, studies about the anti-inflammatory activity of EPS produced by this microorganism still remain limited. The aim of this study was to produce, characterize, and evaluate the anti-inflammatory activity of EPS from K. oxytoca in a pleurisy model. Colorimetric analysis revealed that precipitated crude exopolysaccharides (KEPSC) and deproteinated exopolysaccharides (KEPS) present high levels of total carbohydrates (65.57% and 62.82%, respectively). Analyses of uronic acid (7.90% in KEPSC and 6.21% in KEPS) and pyruvic acid (3.01% in KEPSC and 1.68% in KEPS) confirm that the EPS are acidic. Gas chromatographymass spectrometry analyses demonstrated that the EPS consisted of rhamnose (29.83%), glucose (11.21%), galactose (52.45%), and mannose (6.50%). The treatment of an experimental pleurisy model in rats through subcutaneous administration of 50, 100, 200, and 400 mg/kg of KEPS decreased both the volume of inflammatory exudate and the number of leukocytes recruited to the pleural cavity. The present data showed that EPS production by K. oxytoca using the method described is easy to perform and results in a good yield. In addition, we show that KEPS exhibit anti-inflammatory activity when administered subcutaneously in rats.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Exopolysaccharides from Klebsiella oxytoca: anti-inflammatory activity

Bannwart

Bersani-Amado

Ames

et al. 2022

Braz. J. Pharm. Sci.

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show abstract

“…Based on the results of the GC analysis, it was concluded that the major sugars for this exopolysaccharide were glucose, mannose, rhamnose and N-acetylglucosamine. The remainder of the peaks could probably be the fragments of partially hydrolysed exopolysaccharide or by-product of hydrolysis reaction (Dlamini et al 2007). A limited number of micro-organisms have been reported to produce polysaccharides that yield rhamnose upon hydrolysis.…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharide analysis of biofilm‐forming Candida albicans

Lal

Sharma

Pruthi

et al. 2010

Journal of Applied Microbiology

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Aim: The major objective of the study was to analyse exopolysaccharide produced by a biofilm forming‐clinical strain of Candida albicans. Methods and Results: The biofilm‐forming ability of C. albicans recovered from infected intrauterine devices (IUDs) was evaluated using XTT (2,3‐bis[2‐methoxy‐4‐nitro‐5‐sulfophenyl]‐2H‐tetrazolium‐5‐carboxanilide) reduction assay. The morphological characteristics of the biofilm were assessed using scanning electron microscopy (SEM), atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). Biochemical characterization of the exopolysaccharide was carried out by gel permeation chromatography, gas chromatography (GC), Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. Microscopic studies of C. albicans biofilm revealed complex, heterogeneous three‐dimensional structure, in which yeast cells and hyphal elements were entrenched within exopolysaccharides matrix. Chromatographic analysis data indicated C. albicans exopolysaccharide (c. 300 kDa) to be made up of four major sugar units. The FTIR spectrum revealed specific absorbance of O–H, C–H, O=C=O, C=O, C–N and C–C ring stretching. 1H and 13C NMR data showed the presence of β (1→6) and β (1→3) linkages in the exopolysaccharide chain that were assigned to α‐d‐glucose and β‐d‐glucose, α‐d‐mannose, α‐l‐rhamnose and N‐acetyl glucosamine (β‐d‐GlcNAc), respectively. Conclusions: Study suggested the production of a water soluble c. 300 kDa exopolysaccharide by C. albicans made up of glucose, mannose, rhamnose and N‐acetyl glucosamine subunits. Significance and Impact of the Study: The study could assist in the development of novel therapeutics aimed at disrupting C. albicans biofilms that will translate into improved clearance of Candida‐related infections.

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Isolation of Functional Components β-Glucan and γ-Amino Butyric Acid from Raw and Germinated Barnyard Millet (Echinochloa frumentaceae) and their Characterization

Sharma

Saxena

Riar

2016

Plant Foods Hum Nutr

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The study was carried out to analyze the characteristics of two functional constituents' viz. γ-amino butyric acid (GABA) and β-glucan extracted from raw and germination barnyard millet (var. PRJ-1). A significant (P ≤ 0.05) effect of germination (sprouting) was observed in yield, chemical composition, functional, rheological and antioxidant properties of β-glucan and GABA. The yield of GABA extract was 12.34 % and the content increased from 6.37 mg/100 g in raw to 35.70 mg/100 g in germinated sample. The DPPH, total antioxidant and hydrogen peroxide scavenging activities of GABA extract increased after germination from 45.34 to 65.34 %, 15.3 to 33.3 millimole/g and 38.4 to 64.7 millimole/g, respectively. The yield of β-glucan extract of raw and germinated flour was 6.05 and 5.01 % whereas the β-glucan contents were 83.30 and 79.64 %, respectively. The functional properties of β-glucan i.e., swelling power, water binding capacity and DPPH scavenging activity increased from 1.45 to 1.76 g/g, 2.13 to 2.32 g/g and 44.39 to 57.42 %, respectively, after germination. Similarly there was an increase in the storage modulus after germination process which attributes a better viscoelastic capacity of β-glucan at low frequencies. The results exploit that the β-glucan and GABA might promise a polymeric incipient to be implemented as food additives with variable functional and structural characteristics.

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Characterization of the exopolysaccharide produced by a whey utilizing strain of Klebsiella oxytoca

Cited by 8 publications

References 23 publications

Exopolysaccharides from Klebsiella oxytoca: anti-inflammatory activity

Exopolysaccharides from Klebsiella oxytoca: anti-inflammatory activity

Exopolysaccharide analysis of biofilm‐forming Candida albicans

Isolation of Functional Components β-Glucan and γ-Amino Butyric Acid from Raw and Germinated Barnyard Millet (Echinochloa frumentaceae) and their Characterization

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