Characterization of the expressed immunoglobulin IGHV repertoire in the New World marmoset Callithrix jacchus

Büdingen, Hans-Christian von; Nabavi, Cameron B.; Genain, Claude P.

doi:10.1007/s002510100362

Cited by 15 publications

(15 citation statements)

References 34 publications

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“…The different classes found could also be extrapolated from the tree based on motif pattern within each family, indicating several degrees of specialization in A. nancymaae IGHV at the family level. The different clans defined for human IGHV could be extrapolated in A. nancymaae IGHV (Figure 2) (clan I was formed by IGHV1, IGHV5 and IGHV7, clan II was formed by IGHV2, IGHV4 and IGHV6, and clan III was formed by IGHV3), as has also been observed for other primate IGHV (36, 37).…”

Section: Resultssupporting

confidence: 65%

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Identification of five different IGHV gene families in owl monkeys (Aotus nancymaae)

et al. 2005

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In order to characterize immunoglobulin heavy-chain variable (IGHV) genes in Aotus nancymaae monkeys, different mRNAs encoded by five IGHV families in this non-human primate were molecularly analysed considering their paramount importance in antibody production in an immune response. This study reports gene products exhibiting 91% amino acid similarity with IGHV1, IGHV2, IGHV3, IGHV4 and IGHV7 human IGHV families. Our analyses suggest that the IGHV gene has several conserved characteristics in humans and A. nancymaae. Several amino acid residues that are highly conserved in all family members described in humans were also present in these families in A. nancymaae. Antibody diversity in these families has remained the same since divergence of both species. Our study continues to provide evidence supporting the use of A. nancymaae monkey as an animal model for studying antibody response.

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Section: Resultssupporting

confidence: 65%

“…These characteristics might be common to A. nancymaae , hampering its detection when using our experimental approach. Some families may not be present in non‐human primates' immunoglobulin repertoire (44), as suggested for C. jaccus in the case of the IGHV2 family (36).…”

Section: Discussionmentioning

confidence: 99%

Identification of five different IGHV gene families in owl monkeys (Aotus nancymaae)

et al. 2005

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“…; von Budingen et al . ). As marmosets are a small non‐human primate, they have the advantage of being easier and safer to handle at high containment than larger OWM, but have a high immunologically and physiologically similarity to humans.…”

Section: Introductionmentioning

confidence: 97%

Characterization of lesion formation in marmosets following inhalational challenge with different strains of Burkholderia pseudomallei

Nelson

Núñez

Ngugi

et al. 2015

Int J Experimental Path

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SummaryThe marmoset model of melioidosis was used to explore whether there was any difference in the disease presentation and/or the lesion formation following inhalational challenge with one of four strains of Burkholderia pseudomallei (K96243, 1026b, HBPUB10303a and HBPUB10134a). Marmosets were challenged with a range of bacterial doses and bacterial load, histological and physiological features were determined temporally following lethal disease. Melioidosis presented as an acute, febrile disease with bacteraemia, bacterial dissemination, necrotizing hepatitis, splenitis and pneumonia which was independent of the challenge strain. Generally, there were no major differences in the manifestation of melioidosis following challenge by the different strains of B. pseudomallei; however, there were some differences in the time to death and the severity of the pathological features. The pathological features observed in the liver and spleen of animals challenged with B. pseudomallei strain 1026b were statistically less severe (P < 0.05) and less frequent. However, more severe foci of disease were evident in the lungs of animals challenged with strain 1026b. In all cases, the lesions developed from small areas of bacteria‐infected macrophages surrounded by non‐infected neutrophils into large lesions with both immune cell types infected. The marmoset model was a useful tool enabling the distinction of subtle difference in the pathological response to B. pseudomallei.

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“…However, relatively few studies have examined antibody diversity of non‐human primates, with those that have focusing entirely on primates of biomedical research interest (Von Büdingen et al . ; Druar et al . ; Sundling et al .…”

Section: Discussionmentioning

confidence: 99%

Next‐generation approaches to advancing eco‐immunogenomic research in critically endangered primates

Larsen

Campbell

Yoder

2014

Molecular Ecology Resources

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High-throughput sequencing platforms are generating massive amounts of genomic data from nonmodel species, and these data sets are valuable resources that can be mined to advance a number of research areas. An example is the growing amount of transcriptome data that allow for examination of gene expression in nonmodel species. Here, we show how publicly available transcriptome data from nonmodel primates can be used to design novel research focused on immunogenomics. We mined transcriptome data from the world's most endangered group of primates, the lemurs of Madagascar, for sequences corresponding to immunoglobulins. Our results confirmed homology between strepsirrhine and haplorrhine primate immunoglobulins and allowed for high-throughput sequencing of expressed antibodies (Ig-seq) in Coquerel's sifaka (Propithecus coquereli). Using both Pacific Biosciences RS and Ion Torrent PGM sequencing, we performed Ig-seq on two individuals of Coquerel's sifaka. We generated over 150 000 sequences of expressed antibodies, allowing for molecular characterization of the antigen-binding region. Our analyses suggest that similar VDJ expression patterns exist across all primates, with sequences closely related to the human VH 3 immunoglobulin family being heavily represented in sifaka antibodies. Moreover, the antigen-binding region of sifaka antibodies exhibited similar amino acid variation with respect to haplorrhine primates. Our study represents the first attempt to characterize sequence diversity of the expressed antibody repertoire in a species of lemur. We anticipate that methods similar to ours will provide the framework for investigating the adaptive immune response in wild populations of other nonmodel organisms and can be used to advance the burgeoning field of eco-immunology.

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Characterization of the expressed immunoglobulin IGHV repertoire in the New World marmoset Callithrix jacchus

Cited by 15 publications

References 34 publications

Identification of five different IGHV gene families in owl monkeys (Aotus nancymaae)

Identification of five different IGHV gene families in owl monkeys (Aotus nancymaae)

Characterization of lesion formation in marmosets following inhalational challenge with different strains of Burkholderia pseudomallei

Next‐generation approaches to advancing eco‐immunogenomic research in critically endangered primates

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