Characterization of the GEMINI C18™ Column: Lipophilicity Measurement and LSER

Probing the coordination behavior of Hg2+, CH3Hg+, and Cd2+ towards mixtures of two biological thiols by HPLC-ICP-AES

“…After thermostating the HPLC column to 37°C, individual metals were injected onto the column and detected in the column effluent with an inductively coupled plasma atomic emission spectrometer (ICP-AES). Since the on-column formed metal-thiol complexes are expected to be retained on this C 18 -HPLC column according to their hydrophobicities [40,41], the comparative retention behavior of the investigated metal ions was intended to provide insight into their comparative coordination behavior. Thereafter, the concentration of Cys (ligand 2) in the mobile phase (5.0 mM GSH in PBS-buffer) was gradually increased from 0 to 10 mM Cys (in 0.5 or 1.0 mM steps) and each metal was again injected separately onto the HPLC-ICP-AES system.…”