Probing the coordination behavior of Hg2+, CH3Hg+, and Cd2+ towards mixtures of two biological thiols by HPLC-ICP-AES

Pei, Katie; Sooriyaarachchi, Melani; Sherrell, D.A.; George, Graham N.; Gailer, Jürgen

doi:10.1016/j.jinorgbio.2010.11.019

Cited by 42 publications

(31 citation statements)

References 75 publications

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“…Other metal ions, including Hg 2+ and Pb 2+ , interact with GSH to form linear GS-M-SG complexes. 27 In order to understand the interaction between GSH and Cd 2+ or Hg 2+ , we applied the DFT calculation to obtain the binding energy between GSH and metal ions, including Cd 2+ and Hg 2+ . The binding energy is −420 kcal mol −1 for GSH and Hg 2+ , while the binding energy of GSH and Cd 2+ is −621 kcal mol −1 .…”

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confidence: 99%

Label-Free Colorimetric Detection of Cadmium Ions in Rice Samples Using Gold Nanoparticles

et al. 2014

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A simple and label-free colorimetric method for cadmium ions (Cd 2+ ) detection using unmodified gold nanoparticles (AuNPs) is reported. The unmodified AuNPs easily aggregate in a high concentration of NaCl solution, but the presence of glutathione (GSH) can prevent the saltinduced aggregation of AuNPs. When Cd 2+ is added to the stable mixture of AuNPs, GSH, and NaCl, Cd 2+ can coordinate with 4× GSH as a spherical shaped complex, which decreases the amount of free GSH on the surface of gold nanoparticles to weaken the stability of AuNPs, and AuNPs will easily aggregate in high-salt conditions. On the basis of the mechanism, we design a simple, label-free colorimetric method using AuNPs accompanied by GSH in a high-salt environment to detect Cd 2+ in water and digested rice samples.A s an important natural element, cadmium is widely used in many fields, such as industry, agriculture, and so on. 1 However, chronic exposure of Cd 2+ has caused many serious environmental and health problems, including renal dysfunction, reduced lung capacity, and some cancers. 2,3 In 2013, the government test indicated that some rice samples in South China were contaminated with cadmium. In China, the maximum permitted concentration for cadmium in rice has been set at a level of 0.2 mg/kg, 4 which is lower than the standard of Codex Alimentarius Commission (CAC), but these events are people alarming. Thus, sensitive sensing cadmium in rice is of considerable significance for food safety and human health. Though many methods for Cd 2+ detection have been developed, these methods either are time-consuming or require costly instruments and complicated operation. 5−9 To satisfy the requirement of an on-site and quick testing, a facile, simple, and on-site method for monitoring Cd 2+ is, therefore, still required.Nowadays, many colorimetric detection methods based on gold nanoparticles (AuNPs) or silver nanoparticles have been well developed because these nanoparticles have extremely high extinction coefficients and strong distance-dependent optical properties. 10−17 However, there are very few colorimetric methods for Cd 2+ detection, and the selectivity and sensitivity of those detection methods of Cd 2+ is not sufficiently good for real-world applications. 18−23 For example, a colorimetric method for Cd 2+ detection is developed with triazole-ester modified silver nanoparticles. This method suffers from complex organic synthesis, long response time, and poor sensitivity. 18 AuNPs cofunctionalized with 6-mercaptonicotinic acid and L-cysteine can sense Cd 2+ with a visible color change. However, Pb 2+ and Cu 2+ show interference with the detection of Cd 2+ . 19 A new test paper for the colorimetric immunosensing Cd 2+ has been constructed by using the Cd 2+ -ethylenediaminetetraacetic acid-bovine serum albumin-AuNPs conjugate. The test paper can be successfully used to detect Cd 2+ in drinking and tap water with almost 100% accuracy. However, the practical application is not included in this paper. 22 A new colorimetric met...

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confidence: 99%

Label-Free Colorimetric Detection of Cadmium Ions in Rice Samples Using Gold Nanoparticles

et al. 2014

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“…[17] We therefore replaced the mobile phase thiol Cys -which was used in the previously developed separation -by N-Cys and left all other parameters unchanged (the mobile phase pH was pH 7.4 instead of 7.5). As expected, this increased the retention of Hg 2+ , but the retention of CH 3 Hg + also increased considerably.…”

Section: Retention Behavior Of Hg 2+ and Ch 3 Hg + With Cys/n-cys Conmentioning

confidence: 99%

“…On the other hand, the general approach of injecting a metal species onto a RP-HPLC column that is equilibrated with a mobile phase that contains one or more ligands has already been employed to gain insight into biochemically relevant interactions between a metal ion with multiple ligands under near physiological conditions. [17] HPLC separation of Hg 2+ and CH 3 Hg + under optimized separation conditions…”

Section: Retention Behavior Of Hg 2+ and Ch 3 Hg + With Cys/n-cys Conmentioning

confidence: 99%

Improved RP-HPLC separation of Hg²⁺and CH₃Hg⁺using a mixture of thiol-based mobile phase additives

Meers

Jahromi

Heyne

et al. 2012

Journal of Environmental Science and Health, Part A

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Hg(2+) and CH(3)Hg(+) are frequently encountered in the environment either as free ions or complexed with organic matter, such as humic acids. The majority of the reported HPLC-based separations of environmental mercury species, however, separate Hg(2+) from CH(3)Hg(+) in which the former species elutes close to the void volume. To detect mercury-species in environmental waters that may have so far escaped detection, a separation method is needed that sufficiently retains both Hg(2+) and CH(3)Hg(+). One way to develop such a method is to increase the retention of Hg(2+) and CH(3)Hg(+) using existing HPLC separations. We here report on the improvement of a previously reported RP-HPLC-based separation of Hg(2+) and CH(3)Hg(+) that employed a 100 % aqueous mobile phase [10 mM L-cysteine (Cys) in 50 mM phosphate buffer (pH 7.5)]. To increase the retention of Hg(2+), Cys was replaced by the comparatively more hydrophobic N-acetylcysteine (N-Cys). To achieve a compromise between an increased retention of Hg(2+) and its baseline separation from CH(3)Hg(+) in the shortest possible analysis time, the retention behavior of both mercurials was investigated on two RP-HPLC columns with mobile phases that contained mixtures of Cys and N-Cys in which the overall thiol concentration was maintained at 10 mM. An optimal separation of both mercurials could be achieved in ∼540 s using a Gemini C(18) HPLC column (150 × 4.6 mm I.D.) and a mobile phase comprised of 7.5 mM N-Cys and 2.5 Cys in 50 mM phosphate buffer (pH 7.4). Coupling the developed HPLC separation with an inductively coupled plasma mass spectrometer should allow one to detect mercury species other than Hg(2+) and CH(3)Hg(+) in environmental waters. The detection of such species is critical to better understand the mobilization of mercury species from natural and anthropogenic pollution sources.

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“…Mercury and the organometallic compounds formed from this element have a high affinity for thiol groups of endogenous proteins and metabolites [5]. Thus, they are invariably found in cell, tissues and biological fluids bound to thiol-containing entities, including reduced glutathione (GSH) or cysteine (Cys) [6,7]. The binding of these two mercury species to Cys and/or GSH likely plays an important role in the intracellular transport and excretion of these toxic metal ions [6].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, they are invariably found in cell, tissues and biological fluids bound to thiol-containing entities, including reduced glutathione (GSH) or cysteine (Cys) [6,7]. The binding of these two mercury species to Cys and/or GSH likely plays an important role in the intracellular transport and excretion of these toxic metal ions [6]. Exposure to inorganic mercury may occur by ingestion, inhalation and dermal contact, especially in areas released from mining, smelting, and industrial discharge, where inorganic Hg is a much more common pollutant in the environment than other mercury species.…”

Section: Introductionmentioning

confidence: 99%

Combination of direct infusion mass spectrometry and gas chromatography mass spectrometry for toxicometabolomic study of red blood cells and serum of mice Mus musculus after mercury exposure

García-Sevillano

García-Barrera

Navarro

et al. 2015

Journal of Chromatography B

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Probing the coordination behavior of Hg2+, CH3Hg+, and Cd2+ towards mixtures of two biological thiols by HPLC-ICP-AES

Cited by 42 publications

References 75 publications

Label-Free Colorimetric Detection of Cadmium Ions in Rice Samples Using Gold Nanoparticles

Label-Free Colorimetric Detection of Cadmium Ions in Rice Samples Using Gold Nanoparticles

Improved RP-HPLC separation of Hg²⁺and CH₃Hg⁺using a mixture of thiol-based mobile phase additives

Combination of direct infusion mass spectrometry and gas chromatography mass spectrometry for toxicometabolomic study of red blood cells and serum of mice Mus musculus after mercury exposure

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Probing the coordination behavior of Hg2+, CH3Hg+, and Cd2+ towards mixtures of two biological thiols by HPLC-ICP-AES

Cited by 42 publications

References 75 publications

Label-Free Colorimetric Detection of Cadmium Ions in Rice Samples Using Gold Nanoparticles

Label-Free Colorimetric Detection of Cadmium Ions in Rice Samples Using Gold Nanoparticles

Improved RP-HPLC separation of Hg2+and CH3Hg+using a mixture of thiol-based mobile phase additives

Combination of direct infusion mass spectrometry and gas chromatography mass spectrometry for toxicometabolomic study of red blood cells and serum of mice Mus musculus after mercury exposure

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Improved RP-HPLC separation of Hg²⁺and CH₃Hg⁺using a mixture of thiol-based mobile phase additives