Combination of direct infusion mass spectrometry and gas chromatography mass spectrometry for toxicometabolomic study of red blood cells and serum of mice Mus musculus after mercury exposure

García-Sevillano, M. A.; García-Barrera, Tamara; Navarro, Francisco; Abril, Nieves; Pueyo, Carmen; López-Barea, Juán; Gómez-Ariza, José Luis

doi:10.1016/j.jchromb.2015.01.029

Cited by 16 publications

(8 citation statements)

References 58 publications

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“…Two metabolomics-study approaches have been used to detect metabolites: untargeted and targeted metabolomics 25 . Untarget metabolomics is the comprehensive analysis of all measurable metabolites, and it has been conducted to understand environmental toxin mechanisms 18,[34][35][36][37][38][39] . Biomarker candidates detected in the untargeted analysis can be validated by targeted metabolomics.…”

Section: Metabolomics -Perspectives For Assessing Environmental Exposure To Chemical Agentsmentioning

confidence: 99%

The Use of Metabolomic Tool in Assessing Environmental Exposure

Mendes

André

2021

IJOE

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The impact of the environment on the development of non-communicable chronic diseases has gained prominence in recent years. In this context, a new chemical exposure assessment strategy is needed that is capable of revealing multiple exposures, as well as reflecting the cumulative interaction between such environmental contaminants in the biological system. From this perspective, metabolomics emerges as a promising tool in this field of knowledge, since it is able to identify changes in metabolism and/or gene expression resulting from exposure to environmental factors. The aim of this study was to describe important concepts, as well as the steps that permeate the metabolomics analysis, and also to present some relevant works with the application of metabolomics in the assessment of chemical exposure. A literature review showed a significant increase in the use of metabolomics in environmental toxicology in recent years. This increase is mainly due to advances in analytical techniques and the improvement of data processing tools. However, this field of investigation remains little explored, especially with regard to the study of toxicity associated with chronic exposure to low levels of chemical agents. Thus, it is urgent that omic biomarkers can be used as a tool for decision-making, especially with a view to protecting, diagnosing and recovering human health.

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Section: Metabolomics -Perspectives For Assessing Environmental Exposure To Chemical Agentsmentioning

confidence: 99%

The Use of Metabolomic Tool in Assessing Environmental Exposure

Mendes

André

2021

IJOE

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“…The advantage of this method is that the equipment is simple and the time required is quite short. While the disadvantages include the loss of essential substances, the presence of substances that are not heat resistant and substances containing albumin which cause the withdrawal of these nutritious substances (García-Sevillano et al, 2015).…”

Section: Infusionmentioning

confidence: 99%

Reducing the Effects of Blood Sugar Infusion of Melastoma Malabathricum L. in Mus Musculus

Truong¹

2020

JAMRMHSS

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This study aims to determine the blood sugar-lowering effect of Melastoma malabathricum L. infusion on Mus musculus. The research design used was an experimental laboratory carried out in the pharmacology laboratory of the Ho Chi Minh City University. This study used 15 male mice which were divided into 5 groups consisting of group I as negative control by giving aquadest, groups II, III, IV were given infusion of Melastoma malabathricum L. respectively 0.1 g/ml, 0.2 g/ml, and 0.4 g/ml, and group V as a positive control with glibenclamide suspension. The results showed that statistical testing using the SPSS program showed a significant difference (p <0.05), which means that senggani leaf infusion had a significant effect on reducing blood sugar levels in mice and the most optimal was 0.4 g/ml infusion.

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“…The metabolites were extracted from serum samples using a previously optimized method (García-Sevillano et al 2015b). Briefly, an aliquot of 100 μL of serum was mixed with 500 µL of the extraction solvent MeOH/EtOH (1:1, v/v).…”

Section: Metabolomic Approach For Mice Serum (Mus Musculus) Under Cd/se Exposure Using Gc-ms and Esi-qtof-msmentioning

confidence: 99%

“…Metabolomic analysis by gas chromatographic analysis was also performed using a Trace GC ULTRA gas chromatograph coupled to an ion trap mass spectrometer detector ITQ900, both from Thermo Fisher Scientific (Bremen, Germany) (Table S3 of supplementary material). Operational conditions of Tables S2 and S3 have been previously optimized and described elsewhere (González-Domínguez et al 2015;García-Sevillano et al 2015b).…”

Section: Metabolomic Approach For Mice Serum (Mus Musculus) Under Cd/se Exposure Using Gc-ms and Esi-qtof-msmentioning

confidence: 99%

Metabolic impairments, metal traffic, and dyshomeostasis caused by the antagonistic interaction of cadmium and selenium using organic and inorganic mass spectrometry

Rodríguez-Moro

Roldán

Baya-Arenas

et al. 2019

Environ Sci Pollut Res

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Cadmium (Cd) has become one of the most important environmental pollutants in the world, derived from natural and industrial sources, which is known to be accumulated in the human body, producing serious health effects. On the other hand, Selenium (Se) is an essential element for mammals, which is well known for its antagonistic interaction against Cd toxicity, such as the prevention of oxidative stress induced by this element. For this reason, the use of complementary analytical methods to study the homeostasis of metals, "traffic" between different organs and massive information about metabolites altered by the exposure is of great interest. To this end, a metabolomic workflow based on the use of direct infusion mass spectrometry (DIMS) and gas chromatography mass spectrometry (GC-MS) was applied in mice serum. On the other hand, metals homeostasis and traffic between different organs and serum of mice exposed to Cd and Se, have been evaluated by determining the concentration of metals by inductively coupled plasma mass spectrometry. This work, demonstrate for the first time that Cd exposure causes a decrease of all the elements studied in lung except itself. On the other hand, Se provokes As trafficking from metabolically less active organs (brain, lung and testes) to others with greater metabolic activity (kidney), which also facilitates its excretion. Moreover, when mice are only exposed to Se, it provokes the accumulation of almost all the elements in kidney, except Cd that increases also in liver and brain. However, when both elements are simultaneously administered, Se increases Cd concentration in all the organs except in serum and especially in testis. On the other hand, important metabolic alterations have been detected in the energy and amino acid metabolism, as well as degradation of phospholipidic membranes, and in free fatty acids. In summary, the results show the high potential of the combined use of organic and inorganic mass spectrometry to establish Cd and Se interaction, the biological impairments caused and to provide information about metals traffic and metabolomic changes in exposure experiments.

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Combination of direct infusion mass spectrometry and gas chromatography mass spectrometry for toxicometabolomic study of red blood cells and serum of mice Mus musculus after mercury exposure

Cited by 16 publications

References 58 publications

The Use of Metabolomic Tool in Assessing Environmental Exposure

The Use of Metabolomic Tool in Assessing Environmental Exposure

Reducing the Effects of Blood Sugar Infusion of Melastoma Malabathricum L. in Mus Musculus

Metabolic impairments, metal traffic, and dyshomeostasis caused by the antagonistic interaction of cadmium and selenium using organic and inorganic mass spectrometry

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