Characterization of the hemodynamic response in the rat lumbar spinal cord using intrinsic optical imaging and laser speckle

Brieu, Nicolas; Beaumont, Éric; Dubeau, Simon; Cohen‐Adad, Julien; Lesage, Frédéric

doi:10.1016/j.jneumeth.2010.06.012

Cited by 15 publications

(13 citation statements)

References 50 publications

(59 reference statements)

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“…Moreover, the control group lost the ability to respond to peripheral stimulation by day 7 post-SCI, since the neurovascular amplitudes recorded were very low and showed similar values between 1 and 3 MT. In fact, in healthy (non-SCI) rats, neurovascular response amplitudes are proportional to stimulation intensity, 14 as in the FES group in this study. These findings reveal that FES has the potential to preserve the neurovascular responses in the lumbar spinal cord when stimulating the instrumented hind limb muscles.…”

Section: Plasticity In the Lumbar Spinal Cordmentioning

confidence: 52%

“…Maximal LFP and IOI amplitudes were observed exactly at the same location in the lumbar spinal cord, which is consistent with our previous reports. 14,15 Recording in the somatosensory cortex IOI responses were recorded simultaneously from both sides of the somatosensory cortex during stimulation of the left hind limb muscles. Since the SCI lesion was symmetrical at T10, we stimulated the left muscles to discriminate ipsilateral and contralateral responses at the level of the somatosensory cortex, as depicted in Fig.…”

Section: Neuronal Activity Recording In the Lumbar Spinal Cordmentioning

confidence: 99%

“…Since the spinal cord lesion location and severity in well controlled in rats, it reduces this important bias and thus improve the data interpretation. The originality of this experiment stems from the fact that neurovascular activation in the CNS is examined using an in vivo intrinsic optical imaging (IOI) and electrophysiological techniques [14][15][16] to investigate changes in neurovascular responses post-FES therapy that is performed during the acute phase (i.e. from day 2 to day 6 post-SCI).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functional electrical stimulation post-spinal cord injury improves locomotion and increases afferent input into the central nervous system in rats

Beaumont

Guevara

Dubeau

et al. 2013

The Journal of Spinal Cord Medicine

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Section: Plasticity In the Lumbar Spinal Cordmentioning

confidence: 52%

Section: Neuronal Activity Recording In the Lumbar Spinal Cordmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functional electrical stimulation post-spinal cord injury improves locomotion and increases afferent input into the central nervous system in rats

Beaumont

Guevara

Dubeau

et al. 2013

The Journal of Spinal Cord Medicine

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“…40, and the reflectance values were corrected for the spectral response of the CCD camera and convolved with the LEDs' spectral power distribution. 41 Images of each chromophore (HbO 2 , HbR) were spatially smoothed with a Gaussian kernel of 11 × 11 pixels (0.6× 0.6 mm) with a 5-pixel standard deviation (0.3 mm). An anatomical image was recorded with illumination at 525 nm, and then the region corresponding to brain was manually selected using a closed spline curve to create a brain mask.…”

Section: Optical Imaging Of Intrinsic Signalsmentioning

confidence: 99%

Optical imaging of acute epileptic networks in mice

Guevara¹,

Pouliot

Nguyen

et al. 2013

J. Biomed. Opt

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Abstract. The potential of intrinsic optical imaging and resting-state analysis under anesthetized conditions as a tool to study brain networks associated with epileptic seizures is investigated. Using an acute model of epileptiform activity, the 4-aminopyridine model in live mice, we observe the changes in resting-state networks with the onset of seizure activity and in conditions of spiking activity. Resting-state networks identified before and after the onset of epileptiform activity show both decreased and increased homologous correlations, with a small dependence on seizure intensity. The observed changes are not uniform across the different hemodynamic measures, suggesting a potential decoupling between blood flow and metabolism in the low-frequency networks. This study supports the need for a more extensive investigation of epileptic networks including more than one independent hemodynamic measurement.

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“…OD values were corrected for the wavelength-dependent response of the CCD sensor and convolved with the LEDs profile (Brieu et al, 2010). …”

Section: Methodsmentioning

confidence: 99%

Altered Functional Connectivity Following an Inflammatory White Matter Injury in the Newborn Rat: A High Spatial and Temporal Resolution Intrinsic Optical Imaging Study

et al. 2017

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Very preterm newborns have an increased risk of developing an inflammatory cerebral white matter injury that may lead to severe neuro-cognitive impairment. In this study we performed functional connectivity (fc) analysis using resting-state optical imaging of intrinsic signals (rs-OIS) to assess the impact of inflammation on resting-state networks (RSN) in a pre-clinical model of perinatal inflammatory brain injury. Lipopolysaccharide (LPS) or saline injections were administered in postnatal day (P3) rat pups and optical imaging of intrinsic signals were obtained 3 weeks later. (rs-OIS) fc seed-based analysis including spatial extent were performed. A support vector machine (SVM) was then used to classify rat pups in two categories using fc measures and an artificial neural network (ANN) was implemented to predict lesion size from those same fc measures. A significant decrease in the spatial extent of fc statistical maps was observed in the injured group, across contrasts and seeds (*p = 0.0452 for HbO2 and **p = 0.0036 for HbR). Both machine learning techniques were applied successfully, yielding 92% accuracy in group classification and a significant correlation r = 0.9431 in fractional lesion volume prediction (**p = 0.0020). Our results suggest that fc is altered in the injured newborn brain, showing the long-standing effect of inflammation.

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Characterization of the hemodynamic response in the rat lumbar spinal cord using intrinsic optical imaging and laser speckle

Cited by 15 publications

References 50 publications

Functional electrical stimulation post-spinal cord injury improves locomotion and increases afferent input into the central nervous system in rats

Functional electrical stimulation post-spinal cord injury improves locomotion and increases afferent input into the central nervous system in rats

Optical imaging of acute epileptic networks in mice

Altered Functional Connectivity Following an Inflammatory White Matter Injury in the Newborn Rat: A High Spatial and Temporal Resolution Intrinsic Optical Imaging Study

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